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ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-glucose
-
13.7% of the activity with UTP
-
r
CTP + alpha-D-glucose 1-phosphate
diphosphate + CDP-alpha-D-glucose
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-galactose
UTP + alpha-D-galactose 1-phosphate
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-mannose
UTP + D-mannose 1-phosphate
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
farnesyl triphosphate + UDP-glucose
farnesyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
-
-
-
?
geranyl triphosphate + UDP-glucose
geranyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
-
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
isopentenyl triphosphate + UDP-glucose
isopentenyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
-
-
-
?
methylenebisphosphonate + UDP-glucose
uridine 5'-(beta,gamma-methylenetriphosphate) + alpha-D-glucose + diphosphate
-
-
-
-
?
monothiodiphosphate + UDP-glucose
UTPbeta,gammaS + alpha-D-glucose 1-phosphate
-
poor substrate, 4.8% of Vmax with diphosphate
UTPbeta,gammaS is chemically unstable and undergoes hydrolysis to UDPbetaS and phosphate, which renders the otherwise reversible reaction irreversible, t1/2 at 25°C: 0.5 min
ir
TTP + alpha-D-glucose 1-phosphate
diphosphate + alpha-TDP-glucose
TTP + alpha-D-glucose 1-phosphate
diphosphate + TDP-glucose
-
-
-
-
r
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
UDP + alpha-D-glucose 1-phosphate
phosphate + UDP-glucose
UDP-D-glucose + diphosphate
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
UDP-glucose + adenosine 5'-tetraphosphate
D-glucose-1-phosphate + adenosyl-5'-pentaphospho-5'-uridine
-
-
-
-
?
UDP-glucose + ATP
D-glucose-1-phosphate + adenosyl-5'-tetraphospho-5'-uridine
-
-
-
-
?
UDP-glucose + CTP
D-glucose-1-phosphate + cytidinyl-5'-tetraphospho-5'-uridine
-
-
-
-
?
UDP-glucose + GTP
D-glucose-1-phosphate + guanosyl-5'-tetraphospho-5'-uridine
-
-
-
-
?
UDP-glucose + guanosine 5'-tetraphosphate
D-glucose-1-phosphate + guanosyl-5'-pentaphospho-5'-uridine
-
-
-
-
?
UDP-glucose + tetrapolyphosphate
uridine-5'-pentaphosphate + D-glucose-1-phosphate
-
-
-
-
?
UDP-glucose + tripolyphosphate
uridine-5'-tetraphosphate + D-glucose-1-phosphate
-
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + alpha-UDP-galactose
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
UDP-D-glucose + diphosphate
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
UTP + D-glucose-1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + D-glucose-1-phosphate
UDP-glucose + diphosphate
UTP + glucose-1-phosphate
UDP-glucose + diphosphate
-
-
-
-
?
additional information
?
-
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
ATP + alpha-D-glucose 1-phosphate
diphosphate + ADP-alpha-D-glucose
-
-
-
-
?
CTP + alpha-D-glucose 1-phosphate
diphosphate + CDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
CTP + alpha-D-glucose 1-phosphate
diphosphate + CDP-alpha-D-glucose
-
-
-
-
?
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
-
no activity
-
-
?
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
-
calf and human liver, poor substrate
-
r
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
-
calf and human liver, poor substrate
-
r
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
-
very low activity, wild-type and mutant W218
-
r
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
-
18.5% of the activity with UTP
-
r
diphosphate + CDP-glucose
CTP + alpha-D-glucose 1-phosphate
-
no activity
-
-
?
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
-
no activity
-
-
?
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
-
calf and human liver, poor substrate
-
-
r
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
-
calf and human liver, poor substrate
-
-
r
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
-
14.3% of the activity with UTP
-
r
diphosphate + GDP-glucose
GTP + alpha-D-glucose 1-phosphate
-
no activity
-
-
?
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
no activity
-
-
?
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
calf and human liver
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
calf and human liver
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
very low activity, wild-type and mutant W218
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
poor substrate
-
r
diphosphate + TDP-glucose
TTP + alpha-D-glucose 1-phosphate
-
-
-
-
?
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
?
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
?
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
-
r
diphosphate + UDP-glucose
UTP + alpha-D-glucose 1-phosphate
-
-
-
-
?
diphosphate + UDP-mannose
UTP + D-mannose 1-phosphate
-
calf and human liver, poor substrate
-
-
r
diphosphate + UDP-mannose
UTP + D-mannose 1-phosphate
-
calf and human liver, poor substrate
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
reaction at 10% the rate of UDP-glucose or UDP-galactose pyrophosphorolysis
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
poor substrate
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
calf and human liver
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
-
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
poor substrate
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
calf and human liver
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
poor substrate
-
-
r
diphosphate + UDP-xylose
UTP + D-xylose 1-phosphate
-
calf and human liver
-
-
r
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-alpha-D-glucose
-
-
-
-
?
TTP + alpha-D-glucose 1-phosphate
diphosphate + alpha-TDP-glucose
-
-
-
?
TTP + alpha-D-glucose 1-phosphate
diphosphate + alpha-TDP-glucose
-
-
-
?
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
-
-
-
r
TTP + alpha-D-glucose 1-phosphate
TDP-glucose + diphosphate
-
-
-
r
UDP + alpha-D-glucose 1-phosphate
phosphate + UDP-glucose
-
-
-
?
UDP + alpha-D-glucose 1-phosphate
phosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + alpha-UDP-galactose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + alpha-UDP-galactose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-alpha-D-galactose
-
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
at the same rate as UDP-glucose
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
about 15% of the activity with UDP-glucose
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
reverse reaction: calf and human liver
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
no activity
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
reaction at 5% the rate of UDP-glucose synthesis
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
reverse reaction: calf and human liver
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
1-12% of the activity with UDP-glucose
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
low activity
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
no activity
-
-
?
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
equilibrium dialysis: 8 mol UDP-galactose bound per mol enzyme
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
3.5% of the activity with UDP-glucose
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
-
r
UTP + alpha-D-galactose 1-phosphate
diphosphate + UDP-galactose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
UGP-1 is specifically active toward UTP with alpha-D-glucose 1-phosphate, but displays very low or no activity toward other nucleotides, with either alpha-D-glucose 1-phosphate or alpha-D-mannose 1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
enzyme is highly specific for UTP and glucose 1-phosphate
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
highly specific for UTP
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reverse reaction: highly specific for UDP-glucose
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is induced during amoeba encystment
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
highly specific for UTP
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reverse reaction: highly specific for UDP-glucose
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is induced during amoeba encystment
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
Acetabularia sp.
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reaction at the same rate as UDP-galactose
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
specific for alpha-D-glucose 1-phosphate
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
central reaction in galactose and trehalose metabolism
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
the soluble enzyme shows an apparent higher affinity for and a 2fold higher activity with alpha-D-glucose 1-phosphate than UTP
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reaction critical to mono- and polysaccharide biosynthesis
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
MN788154
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
MN788154
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
specific for alpha-D-glucose 1-phosphate
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
highly specific for UTP
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
key enzyme in biosynthesis of sucrose, cellulose, and other saccharides
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
cumulative feedback regulation in vivo, modulates the flow of carbon into pathways leading to cell wall polysaccharide synthesis
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
specific for alpha-D-glucose 1-phosphate
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
enzyme is highly specific for both UTP and glucose 1-phosphate
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
enzyme is highly specific for both UTP and glucose 1-phosphate
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
equilibrium dialysis: 8 mol UDP-glucose bound per mol enzyme
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reverse reaction: highly specific for UDP-glucose
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
highly specific for UTP
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is essential for cell viability
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
Saccharomyces fragilis
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
highly specific for UTP
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reverse reaction: highly specific for UDP-glucose
specific for UDP-glucose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is specific for UTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is specific for UTP
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
involved in exopolysaccharide biosynthesis
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
involved in exopolysaccharide biosynthesis
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
the multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.24, glucose-1-phosphate thymidylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
the multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.24, glucose-1-phosphate thymidylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + N-acetylglucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dGTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate guanylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
Q94B70
14.2% activity with galactose-1-phosphate
-
-
?
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
in the presence of a divalent metal cation (mainly Mg2+)
-
-
r
UTP + alpha-D-glucose 1-phosphate
UDP-glucose + diphosphate
in the presence of a divalent metal cation (mainly Mg2+)
-
-
r
UTP + D-glucose-1-phosphate
UDP-glucose + diphosphate
-
-
-
-
?
UTP + D-glucose-1-phosphate
UDP-glucose + diphosphate
-
-
-
-
?
UTP + D-glucose-1-phosphate
UDP-glucose + diphosphate
-
-
-
-
r
UTP + D-glucose-1-phosphate
UDP-glucose + diphosphate
-
-
-
-
?
additional information
?
-
-
no activity with ADP-glucose
-
-
?
additional information
?
-
-
no activity with ATP, CTP, and GTP
-
-
?
additional information
?
-
-
no activity with ADP-glucose
-
-
?
additional information
?
-
-
no activity with ATP, CTP, and GTP
-
-
?
additional information
?
-
-
regulation
-
-
?
additional information
?
-
-
UDP-glucose and UDP-galactose pyrophosphorolysis activity in Bifidobacterium bifidum are catalyzed by a single enzyme protein
-
-
?
additional information
?
-
-
UDP-glucose and UDP-galactose pyrophosphorolysis activity in Bifidobacterium bifidum are catalyzed by a single enzyme protein
-
-
?
additional information
?
-
the activity of UgpA toward glucose 1-phosphate is about 33fold higher than that toward galactose 1-phosphate
-
-
?
additional information
?
-
the activity of UgpA toward glucose 1-phosphate is about 33fold higher than that toward galactose 1-phosphate
-
-
?
additional information
?
-
no substrate: alpha-D-glalctose 1-phosphate
-
-
?
additional information
?
-
-
TTP is a poor substrate
-
-
?
additional information
?
-
-
involved in synthesis of hemagglutinin, sulfated proteoglycans, and glycogen in cartilage
-
-
?
additional information
?
-
no substrates: ATP, GTP, nor CTP
-
-
?
additional information
?
-
-
no substrates: ATP, GTP, nor CTP
-
-
?
additional information
?
-
no substrates: ATP, GTP, nor CTP
-
-
?
additional information
?
-
-
the enzyme catalyses a freely reversible reaction and is specific for alpha-D-glucose 1-phosphate
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphate
-
-
?
additional information
?
-
-
molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphate
-
-
?
additional information
?
-
no substrates: ATP, GTP, CTP, dTTP
-
-
?
additional information
?
-
no substrates: ATP, GTP, CTP, dTTP
-
-
?
additional information
?
-
isoform Ugp1 is required for callose deposition during pollen mother cell meiosis and bridges the apoplastic unloading pathway and pollen development
-
-
?
additional information
?
-
-
isoform Ugp1 is required for callose deposition during pollen mother cell meiosis and bridges the apoplastic unloading pathway and pollen development
-
-
?
additional information
?
-
-
no utilization of the nucleotide diphosphate sugars UDP-Gal, UDP-GlnA, UDP-Man, UDP-Xyl, ADP-Glc, CDP-Glc and TDP-Glc
-
-
?
additional information
?
-
-
UDP-galactose is not a UMP-donor and ADP not a UMP-acceptor
-
-
?
additional information
?
-
-
the bisphosphonates alendronate, pamidronate, clodronate and etidronate are not acceptors of UMP. But UDP-glucose serves as uridylyl donor to triphosphate derivatives of the mevalonate pathway, farnesyl triphosphate, geranyl triphosphate, and isopentenyl triphosphate, with formation of farnesyl-tetraphosphouridine, geranyl-tetraphosphouridine, and isopentenyl-tetraphosphouridine, overview. No activity with farnesyl-, geranyl-, or isopentenyl-diphosphates or with dimethylallyl pyrophosphate
-
-
?
additional information
?
-
-
no activity with ADP-glucose
-
-
?
additional information
?
-
-
no activity with ADP-glucose
-
-
?
additional information
?
-
-
no activity with ATP, CTP, and GTP
-
-
?
additional information
?
-
-
no activity with ADP-glucose
-
-
?
additional information
?
-
-
no activity with ATP, CTP, and GTP
-
-
?
additional information
?
-
-
no activity with TPP
-
-
?
additional information
?
-
no substrates: dTTP, CTP, ATP, and GTP, Glc-1P, GlcNAc-1P, Gal-1P, and Man-1P
-
-
?
additional information
?
-
-
no substrates: dTTP, CTP, ATP, and GTP, Glc-1P, GlcNAc-1P, Gal-1P, and Man-1P
-
-
?
additional information
?
-
the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate
-
-
?
additional information
?
-
-
the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate
-
-
?
additional information
?
-
the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTTP + alpha-D-glucose 1-phosphate
diphosphate + dTDP-alpha-D-glucose
-
-
-
?
UDP + alpha-D-glucose 1-phosphate
phosphate + UDP-glucose
UDP-D-glucose + diphosphate
UTP + alpha-D-glucose 1-phosphate
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
additional information
?
-
UDP + alpha-D-glucose 1-phosphate
phosphate + UDP-glucose
-
-
-
?
UDP + alpha-D-glucose 1-phosphate
phosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-alpha-D-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is induced during amoeba encystment
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is induced during amoeba encystment
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
Acetabularia sp.
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
central reaction in galactose and trehalose metabolism
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
reaction critical to mono- and polysaccharide biosynthesis
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
MN788154
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
MN788154
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
key enzyme in biosynthesis of sucrose, cellulose, and other saccharides
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
cumulative feedback regulation in vivo, modulates the flow of carbon into pathways leading to cell wall polysaccharide synthesis
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
enzyme is essential for cell viability
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
involved in exopolysaccharide biosynthesis
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
involved in exopolysaccharide biosynthesis
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
r
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
-
-
?
UTP + alpha-D-glucose 1-phosphate
diphosphate + UDP-glucose
-
-
activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose
r
additional information
?
-
-
regulation
-
-
?
additional information
?
-
-
involved in synthesis of hemagglutinin, sulfated proteoglycans, and glycogen in cartilage
-
-
?
additional information
?
-
isoform Ugp1 is required for callose deposition during pollen mother cell meiosis and bridges the apoplastic unloading pathway and pollen development
-
-
?
additional information
?
-
-
isoform Ugp1 is required for callose deposition during pollen mother cell meiosis and bridges the apoplastic unloading pathway and pollen development
-
-
?
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0.09 - 10
alpha-D-galactose 1-phosphate
0.01 - 3.28
alpha-D-glucose 1-phosphate
0.19 - 0.83
D-glucose-1-phosphate
0.32
farnesyl triphosphate
-
pH 8.0, 30°C
0.21
geranyl triphosphate
-
pH 8.0, 30°C
0.51
isopentenyl triphosphate
-
pH 8.0, 30°C
1.1
methylenebisphosphonate
-
pH 8.0, 30°C
4.5
tripolyphosphate
-
30°C, pH 8.0
0.26 - 0.42
UDP-galactose
additional information
additional information
-
0.09
alpha-D-galactose 1-phosphate
cosubstrate UTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
10
alpha-D-galactose 1-phosphate
-
pH 7.5, temperature not specified in the publication
0.01
alpha-D-glucose 1-phosphate
-
pH 7.8, 37°C
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
-
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
pH 7.8
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
pH 7.5, 30°C
0.03 - 0.048
alpha-D-glucose 1-phosphate
-
pH 8.5, 37°C
0.031
alpha-D-glucose 1-phosphate
-
pH 7.5, temperature not specified in the publication
0.03224
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
0.033
alpha-D-glucose 1-phosphate
-
pH 7.5, temperature not specified in the publication
0.0364
alpha-D-glucose 1-phosphate
pH 7.5, 37°C
0.04455
alpha-D-glucose 1-phosphate
-
0.04988
alpha-D-glucose 1-phosphate
cosubstrate: dTTP, pH 7.5, 25°C
0.055 - 0.08
alpha-D-glucose 1-phosphate
-
-
0.055 - 0.08
alpha-D-glucose 1-phosphate
-
pH 7.8
0.055 - 0.08
alpha-D-glucose 1-phosphate
-
at physiological substrate concentration, pH 8.5, 37°C
0.06
alpha-D-glucose 1-phosphate
-
0.067
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant E412K
0.074
alpha-D-glucose 1-phosphate
-
pH 8.35,25°C
0.07765
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
0.085
alpha-D-glucose 1-phosphate
wild-type, pH 7.5, 37°C
0.088
alpha-D-glucose 1-phosphate
mutant A8G, pH 7.5, 37°C
0.095
alpha-D-glucose 1-phosphate
-
-
0.095
alpha-D-glucose 1-phosphate
-
pH 7.8
0.122
alpha-D-glucose 1-phosphate
pH 7.2, temperature not specified in the publication, recombinant enzyme
0.13
alpha-D-glucose 1-phosphate
cosubstrate UTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.14
alpha-D-glucose 1-phosphate
UDP-glucose synthesis
0.14 - 0.18
alpha-D-glucose 1-phosphate
-
-
0.14 - 0.18
alpha-D-glucose 1-phosphate
-
pH 8.0, 30°C
0.151
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant K410S
0.155
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant E412Q
0.183
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.192
alpha-D-glucose 1-phosphate
pH 7.8, 25°C
0.198
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant N491P/L492E
0.202
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant P414G/T415P
0.205
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant E412D
0.217
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant V416N
0.219
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant T406K/M407L
0.226
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.6, 37°C
0.235
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant mutant S309N/S311R
0.24
alpha-D-glucose 1-phosphate
Q94B70
-
0.24
alpha-D-glucose 1-phosphate
pH 7.5, 30°C
0.253
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.316
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.36
alpha-D-glucose 1-phosphate
UDP-glucose synthesis
0.36
alpha-D-glucose 1-phosphate
cosubstrate TTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.44
alpha-D-glucose 1-phosphate
pH 7.0, 50°C
0.46
alpha-D-glucose 1-phosphate
-
-
0.68 - 1
alpha-D-glucose 1-phosphate
-
pH 8.0, 37°C
0.68 - 1
alpha-D-glucose 1-phosphate
-
pH 7.2, 30°C
0.68 - 1
alpha-D-glucose 1-phosphate
-
recombinant wild-type enzyme, pH 8.0, 25°C
0.68 - 1
alpha-D-glucose 1-phosphate
-
at substrate concentrations above 2 mM, pH 8.5, 37°C
0.715
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.742
alpha-D-glucose 1-phosphate
pH 7.5, 37°C
0.83
alpha-D-glucose 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.83
alpha-D-glucose 1-phosphate
-
UDP-glucose synthesis
0.9
alpha-D-glucose 1-phosphate
-
pH 9.0, temperature not specified in the publication
1.23
alpha-D-glucose 1-phosphate
wild-type enzyme, pH 7.5, 80°C
1.23
alpha-D-glucose 1-phosphate
-
wild-type enzyme, pH 7.5, 80°C
1.23
alpha-D-glucose 1-phosphate
wild type enzyme, at pH 7.5 and 80°C
1.74
alpha-D-glucose 1-phosphate
mutant enzyme Y97M, pH 7.5, 80°C
1.74
alpha-D-glucose 1-phosphate
-
mutant enzyme Y97M, pH 7.5, 80°C
1.74
alpha-D-glucose 1-phosphate
mutant enzyme Y97M, at pH 7.5 and 80°C
2.5 - 3
alpha-D-glucose 1-phosphate
mutant enzyme Y97N, pH 7.5, 80°C
2.5 - 3
alpha-D-glucose 1-phosphate
-
mutant enzyme Y97N, pH 7.5, 80°C
2.5 - 3
alpha-D-glucose 1-phosphate
mutant enzyme Y97N, at pH 7.5 and 80°C
3.28
alpha-D-glucose 1-phosphate
mutant enzyme Y97V, pH 7.5, 80°C
3.28
alpha-D-glucose 1-phosphate
-
mutant enzyme Y97V, pH 7.5, 80°C
3.28
alpha-D-glucose 1-phosphate
mutant enzyme Y97V, at pH 7.5 and 80°C
0.336
ATP
-
pH 7.6, 70°C
0.276
CTP
-
pH 7.6, 70°C
0.19
D-glucose-1-phosphate
-
-
0.83
D-glucose-1-phosphate
-
-
0.027
diphosphate
Y192A mutant protein
0.03
diphosphate
Ccut-8 mutant protein
0.03
diphosphate
K183A mutant protein
0.03
diphosphate
pyrophosphorolysis
0.034
diphosphate
recombinant wild-type, pH 7.6, 25°C
0.039
diphosphate
wild-type protein
0.04
diphosphate
K332A mutant protein
0.054
diphosphate
-
pH 8.5, 37°C
0.07
diphosphate
pyrophosphorolysis
0.084
diphosphate
-
pH 7.8
0.09
diphosphate
Ncut-21 mutant protein
0.11 - 0.13
diphosphate
-
pH 8.0, 30°C
0.11 - 0.13
diphosphate
-
at physiological substrate concentration, pH 8.5, 37°C
0.17
diphosphate
-
pH 8.35, 25°C
0.18
diphosphate
-
non-galactosemic phenotype, pH 7.8, 37°C
0.2
diphosphate
pH 7.8, 25°C
0.2
diphosphate
-
galactosemic phenotype, pH 7.8, 37°C
0.21
diphosphate
-
pH 7.8
0.24
diphosphate
K405A mutant protein
0.33
diphosphate
-
pH 7.2, 30°C
0.33
diphosphate
-
diphosphate in form of Mg-diphosphate
0.376
diphosphate
pH 7.5, 37°C
0.407
diphosphate
recombinant mutant C99S, pH 7.6, 25°C
0.56
diphosphate
-
pyrophosphorolysis
0.56
diphosphate
-
diphosphate in form of Mg-diphosphate
0.9
diphosphate
Ncut-37 mutant protein
1.66
diphosphate
-
pH 9.0, temperature not specified in the publication
2.4
diphosphate
-
pH 7.6, 37°C
4.9
diphosphate
K260A mutant protein
6.4
diphosphate
Ccut-32 mutant protein
9.8
diphosphate
Ccut-67 mutant protein
29.4
diphosphate
Delta 1-4 mutant protein
33.3
diphosphate
Ccut-101 mutant protein
60
diphosphate
Delta 1-8 mutant protein
0.02422
dTTP
pH 7.5, 25°C
0.366
dTTP
-
pH 7.6, 70°C
0.184
GTP
-
pH 7.6, 37°C
0.58
Mg2+
pyrophosphorolysis direction
0.35
TTP
-
0.9
TTP
S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.26
UDP-galactose
pH 7.5, 30°C
0.42
UDP-galactose
-
pH 7.8
0.00167
UDP-glucose
pH 7.5, temperature not specified in the publication
0.00433
UDP-glucose
pH 7.5, temperature not specified in the publication
0.03
UDP-glucose
Ccut-8 mutant protein
0.03
UDP-glucose
K183A mutant protein
0.03
UDP-glucose
K332A mutant protein
0.031
UDP-glucose
Y192A mutant protein
0.034
UDP-glucose
wild-type protein
0.035
UDP-glucose
-
30°C, pH 8.0
0.04
UDP-glucose
K405A mutant protein
0.04
UDP-glucose
pyrophosphorolysis
0.05 - 0.066
UDP-glucose
-
-
0.05 - 0.066
UDP-glucose
-
-
0.05 - 0.066
UDP-glucose
-
-
0.05 - 0.066
UDP-glucose
-
pH 7.8
0.05 - 0.066
UDP-glucose
-
pH 7.6, 37°C
0.06
UDP-glucose
pyrophosphorolysis
0.065
UDP-glucose
recombinant wild-type, pH 7.6, 25°C
0.074
UDP-glucose
K260A mutant protein
0.101
UDP-glucose
recombinant mutant C99S, pH 7.6, 25°C
0.105
UDP-glucose
pH 7.8, 25°C
0.11
UDP-glucose
pH 7.5, 30°C
0.118
UDP-glucose
Ccut-101 mutant protein
0.12 - 0.18
UDP-glucose
-
pH 8.0, 30°C
0.12 - 0.18
UDP-glucose
-
at physiological substrate concentrations, pH 8.5, 37°C
0.14
UDP-glucose
-
non-galactosemic phenotype, pH 7.8, 37°C
0.18
UDP-glucose
-
galactosemic phenotype, pH 7.8, 37°C
0.19
UDP-glucose
-
pH 8.35, 25°C
0.31
UDP-glucose
Ccut-32 mutant protein
0.362
UDP-glucose
Ccut-67 mutant protein
0.503
UDP-glucose
Delta 1-8 mutant protein
0.555
UDP-glucose
Delta 1-4 mutant protein
0.68
UDP-glucose
-
pyrophosphorolysis
0.774
UDP-glucose
-
pH 7.5, temperature not specified in the publication
1.1
UDP-glucose
Ncut-21 mutant protein
3.04
UDP-glucose
pH 7.5, 37°C
57
UDP-glucose
Ncut-37 mutant protein
0.0075
UTP
-
isoenzyme UgpG, 30°C, pH 7.8
0.025
UTP
-
pH 7.5, temperature not specified in the publication
0.053
UTP
pH 7.2, temperature not specified in the publication, recombinant enzyme
0.058 - 0.07
UTP
-
pH 8.0, 37°C
0.058 - 0.07
UTP
-
pH 7.8, 37°C
0.08
UTP
UDP-glucose synthesis
0.08
UTP
cosubstrate alpha-D-galactose 1-phosphate, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.093
UTP
-
pH 8.35, 25°C
0.1 - 0.2
UTP
-
pH 7.5, 30°C
0.1 - 0.2
UTP
-
pH 8.0, 30°C
0.1 - 0.2
UTP
-
at physiological substrate concentrations, pH 8.5, 37°C
0.14
UTP
S0.5 value, hyperbolic behaviour, pH 8.0, 37°C
0.17
UTP
UDP-glucose synthesis
0.18
UTP
wild-type, pH 7.5, 37°C
0.22
UTP
-
UDP-glucose synthesis
0.22
UTP
-
UTP in form of MgUTP
0.229
UTP
-
isoenzyme RmlA, 30°C, pH 7.8
0.35
UTP
mutant A8G, pH 7.5, 37°C
0.53
UTP
-
at substrate concentrations above 2 mM, pH 8.5, 37°C
0.56
UTP
-
recombinant wild-type enzyme, pH 8.0, 25°C
0.56
UTP
-
UTP in form of MgUTP
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
two-substrate kinetics
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetic parameters of various organisms, pH 8.0, overview
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
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D130A
site-directed mutagenesis the mutant shows highly reduced activity compatred to the wild-type enzyme
R15A
site-directed mutagenesis the mutant shows highly reduced activity compatred to the wild-type enzyme
C123S
-
site-directed mutagenesis, active enzyme, 7fold increase in Km for magnesium diphosphate, 2fold increased Ki for MgUTP2-, no longer sensitive to SH-reagents, e.g. iodoacetamide
D253L
0.063% of wild-type activity
E412D
site-directed mutagenesis, the mutation does not change the oligomeric state, the mutant shows 176% catalytic activity compared to the wild-type enzyme
E412K
site-directed mutagenesis, the mutant has a longer side chain with a reverse in charge showed obvious inhibitory effects which results in 78% reduced activity compared to the wild-type hUGPase activity
E412Q
site-directed mutagenesis, the mutation changes the charge property, but not the length of side chain and shows only a marginal increase in activity of 19% when compared with the wild-type protein
G115D
0.004% of wild-type activity
G116A
0.067% of wild-type activity
H266R
-
site-directed mutagenesis, mutant enzyme is active and similar to the wild-type, 4fold decrease in Km and Ki for MgUTP
H446S
-
site-directed mutagenesis, the mutant shows only slight dissociation and slightly reduced activity in forward and reverse reactionsactivity
I466T
-
site-directed mutagenesis, the mutant shows dissociation of subunits, a tetramer appears in addition to di- and monomers, and highly reduced activity in forward and reverse reactions
I468K
-
site-directed mutagenesis, the mutant shows dissociation of subunits, a tetramer appears in addition to di- and monomers, the mutant shows reduced activity in the reverse reaction
I487D
-
site-directed mutagenesis, the mutant shows dissociation of subunits, and highly reduced activity in forward and reverse reactions
K127A
0.150% of wild-type activity
K396A
0.041% of wild-type activity
K4110S
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
L492E
-
site-directed mutagenesis, the mutant shows only slight dissociation and retains activity
N251L
0.011% of wild-type activity
N391P/L492E
-
site-directed mutagenesis, inactive mutant showing dissociation into di- and monomers
N491P
-
site-directed mutagenesis, the mutant shows reduced activity in the reverse reaction
N491P/L492E
site-directed mutagenesis, mutant N491P/L492E is constructed to depolymerize hUGPase octamers, the mutation in the C-terminal left-handed beta-helix changes the oligomerization state the mutant enzyme, that becomes monomeric, it shows about the double activity of the wild-type enzyme
P414G/T415P
site-directed mutagenesis, the mutant shows activity similar to that of the wild-type hUGPase, the mutation does not change the oligomeric state
R389H
-
site-directed mutagenesis, mutant enzyme is active and similar to the wild-type
R391K
-
site-directed mutagenesis, no activity, no correct folding
R422Q
-
site-directed mutagenesis, mutant enzyme is active and similar to the wild-type
R445H
-
site-directed mutagenesis, mutant enzyme is active and similar to the wild-type
S309N/S311R
site-directed mutagenesis, mutation in sequence analogy to the Saccharomyces cerevisiae enzyme, the mutant shows 84% of wild-type activity
T406K/M407L
site-directed mutagenesis, the mutant shows activity similar to that of the wild-type hUGPase, the mutation does not change the oligomeric state
T448K
-
site-directed mutagenesis, the mutant shows dissociation of subunits, a tetramer appears in addition to di- and monomers, and highly reduced activity in forward and reverse reactions
V416N
site-directed mutagenesis, the mutant shows activity similar to that of the wild-type hUGPase, the mutation does not change the oligomeric state
W218S
-
site-directed mutagenesis, mutant enzyme is active and similar to the wild-type, increase in Km
W333S
-
site-directed mutagenesis, no activity, no correct folding
C99S
site-directed mutagenesis, half Vmax of wild-type, 12fold higher Km for diphosphate, altered diphosphate binding
Ccut-101
101 amino acid residues deleted (exons 16-19, and 1 amino acid residue of exon 15)
Ccut-32
32 amino acid residues deleted (exon 18 and exon 19)
Ccut-67
67 amino acid residues deleted (exons 17-19)
Ccut-8
8 amino acid residues deleted (last exon (exon 19))
Delta 1-4
deletion of 4 amino acid residues of the C-terminal domain, possibly involved in oligomerization
Delta 1-8
deletion of 8 amino acid residues of the C-terminal domain, possibly involved in oligomerization
Delta-NB
deletion mutant: amino acid residue 96-100 deleted (essential for catalysis)
K183A
possibly involved in subunit interaction
K260A
possibly involved in diphosphate binding
K332A
possibly involved in subunit interaction
K405A
possibly involved in subunit interaction
Ncut-21
21 amino acid residues deleted (exon 1)
Ncut-37
37 amino acid residues deleted (exon 1 and exon 2)
Y192A
possibly involved in UDP-glucose binding
H191L
-
no residual activity
H191N
-
0.3% of wild-type activity
K380D
-
no residual activity
K95A
-
0.5% of wild-type activity
L281D
-
16.3% of wild-type activity
D462H
natural mutation of isoform Ugp1, D462 in rice variety B5 is replaced by H462 in rice variety TN1
A8G
mutant exhibits activity only for a combination of UTP and glucose 1-phosphate, similar to wild-type
H497A
-
site-directed mutagenesis, the mutant shows dissociation of subunits, and highly reduced activity in forward and reverse reactions
H497A
-
site-directed mutagenesis, the mutant shows only slight dissociation and retains full activity in the reverse eaction, but shows reduced reaction in the forward reaction
Y97A
specific Glc-1-P UTase activity of the mutant enzyme is 2.3fold lower than the specific activity of the mutant enzyme
Y97A
activity is 2.3fold lower than that of the wild-type enzyme
Y97A
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97C
specific Glc-1-P UTase activity of the mutant enzyme is 1.9fold lower than the specific activity of the mutant enzyme
Y97C
activity is 1.87fold lower than that of the wild-type enzyme
Y97D
specific Glc-1-P UTase activity of the mutant enzyme is 6.2fold lower than the specific activity of the mutant enzyme
Y97D
activity is 6.2fold lower than that of the wild-type enzyme
Y97D
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97E
inactive
Y97E
mutant enzyme shows mutant enzyme shows no activity
Y97F
specific Glc-1-P UTase activity of the mutant enzyme is 1.2fold lower than the specific activity of the mutant enzyme
Y97F
activity is 1.2fold lower than that of the wild-type enzyme
Y97F
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97G
specific Glc-1-P UTase activity of the mutant enzyme is 4.2fold lower than the specific activity of the mutant enzyme
Y97G
activity is 4.2fold lower than that of the wild-type enzyme
Y97G
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97H
specific Glc-1-P UTase activity of the mutant enzyme is 2.6fold higher than the specific activity of the mutant enzyme
Y97H
activity is 1.6fold lower than that of the wild-type enzyme
Y97H
the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97I
inactive
Y97I
mutant enzyme shows no activity
Y97I
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97K
specific Glc-1-P UTase activity of the mutant enzyme is 3.7fold lower than the specific activity of the mutant enzyme
Y97K
activity is 3.7fold lower than that of the wild-type enzyme
Y97K
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97L
specific Glc-1-P UTase activity of the mutant enzyme is 2.4fold higher than the specific activity of the mutant enzyme
Y97L
activity is 2.4fold higher than that of the wild-type enzyme
Y97L
the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97M
specific Glc-1-P UTase activity of the mutant enzyme is 2.8fold higher than the specific activity of the mutant enzyme
Y97M
activity is 2.8fold higher than that of the wild-type enzyme
Y97M
the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97N
specific Glc-1-P UTase activity of the mutant enzyme is 4.1fold higher than the specific activity of the mutant enzyme
Y97N
activity is 4.1fold higher than that of the wild-type enzyme
Y97N
the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97Q
specific Glc-1-P UTase activity of the mutant enzyme is 1.04fold higher than the specific activity of the mutant enzyme
Y97Q
activity is 1.04fold higher than that of the wild-type enzyme
Y97Q
the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97R
inactive
Y97R
mutant enzyme shows no activity
Y97S
specific Glc-1-P UTase activity of the mutant enzyme is 1.1fold higher than the specific activity of the mutant enzyme
Y97S
activity is 1.1fold higher than that of the wild-type enzyme
Y97S
the mutant shows increased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97T
specific Glc-1-P UTase activity of the mutant enzyme is 4fold lower than the specific activity of the mutant enzyme
Y97T
activity is 3.96fold lower than that of the wild-type enzyme
Y97T
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97V
specific Glc-1-P UTase activity of the mutant enzyme is 3.34fold lower than the specific activity of the mutant enzyme
Y97V
activity is 3.34fold lower than that of the wild-type enzyme
Y97V
the mutant shows decreased glucose-1-phosphate uridylyltransferase activity compared to the wild type enzyme
Y97W
inactive
Y97W
mutant enzyme shows no activity
additional information
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enzyme gene disruption mutant reduces the survival of Aeromonas hydrophila in serum to less than 1%, decreases the ability of strains to adhere and reduces by 1.5 or 2 log units the virulence of Aeromonas serotype O34 strains in a septicaemia model. The mutant shows two types of lipopolysaccharide structures. The first one corresponds to a rough strain having the complete core, but lacking the terminal galactose residue from the LPS-core and 4-amino-4-deoxyarabinose residues from phosphate groups in lipid A. The second one corresponds to a deeply truncated structure with the LPS-core restricted to one 3-deoxy-D-manno-oct-2-ulosonic acid and three L-glycero-D-manno-heptose residues
additional information
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construction of transgenic plants, with increased or deleted expression of enzyme, study of sucrose effects on the enzyme and gene Ugp, respectively
additional information
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UGP2 is unable to replace UGP1 in UGP1 knockout lines
additional information
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UGP2 is unable to replace UGP1 in UGP1 knockout lines
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additional information
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truncation mutant DELTA 490-497 is almost inactive due to dissociation into di- and monomers
additional information
amino acid exchanges in hydrophobic domain
additional information
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amino acid exchanges in hydrophobic domain
additional information
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overexpression of sucrose synthase and of UDP-glucose pyrophosphorylase. Single transgenic plants show significant increases in the height growth. Double-transgene plants demonstrate an additive effect and are even taller than single-transgene parents. Double-transgene plants may show increases in soluble sugar content. Both enzymes may be markers for sink strength
additional information
isoform Ugp1 silencing by RNAi results in male sterility of plants. Pollen mother cells of silenced plants appear normal before meiosis, but during meiosis, normal callose deposition is disrupted and the degeneration of the tapetum and middle layer is inhibited
additional information
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isoform Ugp1 silencing by RNAi results in male sterility of plants. Pollen mother cells of silenced plants appear normal before meiosis, but during meiosis, normal callose deposition is disrupted and the degeneration of the tapetum and middle layer is inhibited
additional information
generation of gain-of-function promoter-GUS fusion constructs, overview
additional information
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generation of gain-of-function promoter-GUS fusion constructs, overview
additional information
the UTP-glucose-1-phosphate uridylyltransferase activity of mutant enzyme DC005 is reduced by 10%, as compared to that of the wild-type ST0452 protein, while the activity of DC011 is increased by 18%, indicating that the enzymatic activity in the N-terminal region is not greatly affected by truncation of the C-terminal 5 or 11 residues. The mutant enzyme DC011 (deletion of the C-terminal 11 residues of the ST0452 protein) shows little thermal stability at 80°C. The C-terminal domain of the ST0452 protein, with its LbetaH structure, appears to be essential for the formation of its trimeric form and, in turn, the high stability of the entire ST0452 protein. The deletion mutant enzymes DC021, DC031, DC041, DC071 and DC121, are produced in an insoluble form or aggregated immediately after purification. Mutant enzymes DC051 and DC171 can be expressed in a soluble form. Mutant enzyme DC051 becomes completely insoluble after 5 min treatment at 60°C, while mutant enzyme DC171 is insoluble after 5 min treatment at 70 °C
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