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Literature summary for 2.7.7.9 extracted from

  • Okazaki, Y.; Shimojima, M.; Sawada, Y.; Toyooka, K.; Narisawa, T.; Mochida, K.; Tanaka, H.; Matsuda, F.; Hirai, A.; Hirai, M.Y.; Ohta, H.; Saito, K.
    A chloroplastic UDP-glucose pyrophosphorylase from Arabidopsis is the committed enzyme for the first step of sulfolipid biosynthesis (2009), Plant Cell, 21, 892-909.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.9

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli C41(DE3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
diphosphate with 0.25 mM 57% activity, with 1 mM 11% activity, with 10 mM 1.5% activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 UTP
-
 Arabidopsis thaliana
0.24
-
 alpha-D-glucose 1-phosphate
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for activity Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94B70
-
-

Purification (Commentary)

Purification (Comment) Organism
metal chelation chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate 14.2% activity with galactose-1-phosphate Arabidopsis thaliana UDP-glucose + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucose pyrophosphorylase UGP3
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana phosphate starvation up

General Information

General Information Comment Organism
metabolism involved in sulfolipid synthesis Arabidopsis thaliana