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Literature summary for 2.7.7.9 extracted from
- Expression, purification, and characterization of a functionally active Mycobacterium tuberculosis UDP-glucose pyrophosphorylase (2008), Protein Expr. Purif., 61, 50-56.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged version expressed in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01187 | - |
UTP | - |
Mycobacterium tuberculosis |  |
| 0.04455 | - |
alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Mycobacterium tuberculosis | 5618 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely required | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
monomer and different oligomers, gel filtration, x * 36000, SDS-PAGE, x * 35827.0, MALDI-TOF (calculated: 35938.8) | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | O05576 | - |
- |
| Mycobacterium tuberculosis H37Rv | O05576 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal affinity chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1005 | - |
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + UDP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | monomer and different oligomers, gel filtration, x * 36000, SDS-PAGE, x * 35827.0, MALDI-TOF (calculated: 35938.8) | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose pyrophosphorylase | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min treatment: about 15% activity | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 55.03 | - |
alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | |
| 93.44 | - |
UTP | - |
Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Mycobacterium tuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | involved in cell envelope precursor synthesis | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1240 | - |
alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | |
| 7870 | - |
UTP | - |
Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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