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Literature summary for 2.7.7.9 extracted from

  • Ebrecht, A.C.; Asencion Diez, M.D.; Piattoni, C.V.; Guerrero, S.A.; Iglesias, A.A.
    The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate (2015), Biochim. Biophys. Acta, 1850, 88-96.
    View publication on PubMed
Search for more literature for 2.7.7.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
H2O2 complete loss of activity after 30 min, inactivation is reverted by reduction by cysteine and thioredoxin Giardia intestinalis
sodium nitroprusside complete loss of activity after 30 min, inactivation is reverted by reduction by cysteine and thioredoxin Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 UTP cosubstrate alpha-D-galactose 1-phosphate, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C Giardia intestinalis
0.09
-
 alpha-D-galactose 1-phosphate cosubstrate UTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C Giardia intestinalis
0.13
-
 alpha-D-glucose 1-phosphate cosubstrate UTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C Giardia intestinalis
0.14
-
 UTP S0.5 value, hyperbolic behaviour, pH 8.0, 37°C Giardia intestinalis
0.36
-
 alpha-D-glucose 1-phosphate cosubstrate TTP, S0.5 value, hyperbolic behaviour, pH 8.0, 37°C Giardia intestinalis
0.9
-
 TTP S0.5 value, hyperbolic behaviour, pH 8.0, 37°C Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strict dependence on Mg2+ Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48300
-
-
 Giardia intestinalis
50000
-
 gel filtration Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis A8BJJ7
-
-
Giardia intestinalis ATCC 50803 A8BJJ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: ATP, GTP, nor CTP Giardia intestinalis ?
-
 ?
additional information no substrates: ATP, GTP, nor CTP Giardia intestinalis ATCC 50803 ?
-
 ?
TTP + alpha-D-glucose 1-phosphate
-
 Giardia intestinalis diphosphate + alpha-TDP-glucose
-
 ?
TTP + alpha-D-glucose 1-phosphate
-
 Giardia intestinalis ATCC 50803 diphosphate + alpha-TDP-glucose
-
 ?
UTP + alpha-D-galactose 1-phosphate
-
 Giardia intestinalis diphosphate + alpha-UDP-galactose
-
 ?
UTP + alpha-D-galactose 1-phosphate
-
 Giardia intestinalis ATCC 50803 diphosphate + alpha-UDP-galactose
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Giardia intestinalis diphosphate + UDP-alpha-D-glucose
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Giardia intestinalis ATCC 50803 diphosphate + UDP-alpha-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48300, calculated, 1 * 50000, SDS-PAGE Giardia intestinalis