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Literature summary for 2.7.7.9 extracted from

  • Maeda, K.; Narikawa, R.; Ikeuchi, M.
    CugP is a novel ubiquitous non-GalU-type bacterial UDP-glucose pyrophosphorylase found in cyanobacteria (2014), J. Bacteriol., 196, 2348-2354.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
A8G mutant exhibits activity only for a combination of UTP and glucose 1-phosphate, similar to wild-type Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.5, 37°C Synechocystis sp.
0.088
-
 alpha-D-glucose 1-phosphate mutant A8G, pH 7.5, 37°C Synechocystis sp.
0.18
-
 UTP wild-type, pH 7.5, 37°C Synechocystis sp.
0.35
-
 UTP mutant A8G, pH 7.5, 37°C Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P74285
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: dTTP, CTP, ATP, and GTP, Glc-1P, GlcNAc-1P, Gal-1P, and Man-1P Synechocystis sp. ?
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Synechocystis sp. diphosphate + UDP-alpha-D-glucose enzyme is highly specific for UTP and glucose 1-phosphate ?

Synonyms

Synonyms Comment Organism
CugP
-
 Synechocystis sp.
cyanobacterial UDP-Glc PPase
-
 Synechocystis sp.
sll1558
-
 Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.5, 37°C Synechocystis sp.
0.47
-
 UTP wild-type, pH 7.5, 37°C Synechocystis sp.
0.48
-
 UTP mutant A8G, pH 7.5, 37°C Synechocystis sp.
0.48
-
 alpha-D-glucose 1-phosphate mutant A8G, pH 7.5, 37°C Synechocystis sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.38
-
 UTP mutant A8G, pH 7.5, 37°C Synechocystis sp.
2.67
-
 UTP wild-type, pH 7.5, 37°C Synechocystis sp.
5.33
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.5, 37°C Synechocystis sp.
5.5
-
 alpha-D-glucose 1-phosphate mutant A8G, pH 7.5, 37°C Synechocystis sp.