Literature summary for 2.7.7.9 extracted from

Thoden, J.B.; Holden, H.M.
Active site geometry of glucose-1-phosphate uridylyltransferase (2007), Protein Sci., 16, 1379-1388.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with both Mg2+ and UDP-glucose. Residues involved in anchoring the ligand to the active site include the polypeptide chain backbone atoms of Ala20, Gly21, Gly117, Gly180, and Ala214, and the side chains of Glu36, Gln112, Asp143, Glu201, and Lys202. Two magnesium ions are coordinated to the UDP-glucose. An alpha- and a beta-phosphoryl oxygen, three waters, and the side chain of Asp142 ligate the first Mg2+ ion, whereas the second ion is coordinated by an alpha-phosphoryl oxygen and five waters	Corynebacterium glutamicum
in presence of both magnesium and UDP-glucose. Residues anchoring the ligand to the acitve site include polypepetide backbone atoms of A20, G21, G117, G180 and A214 and side chain residues of E36, Q112, D143, E201, and K202. Two magnesium ions coordinate to UDP-glucose	Corynebacterium glutamicum

Crystallization (Comment)

Organism

in complex with both Mg2+ and UDP-glucose. Residues involved in anchoring the ligand to the active site include the polypeptide chain backbone atoms of Ala20, Gly21, Gly117, Gly180, and Ala214, and the side chains of Glu36, Gln112, Asp143, Glu201, and Lys202. Two magnesium ions are coordinated to the UDP-glucose. An alpha- and a beta-phosphoryl oxygen, three waters, and the side chain of Asp142 ligate the first Mg2+ ion, whereas the second ion is coordinated by an alpha-phosphoryl oxygen and five waters

Corynebacterium glutamicum

in presence of both magnesium and UDP-glucose. Residues anchoring the ligand to the acitve site include polypepetide backbone atoms of A20, G21, G117, G180 and A214 and side chain residues of E36, Q112, D143, E201, and K202. Two magnesium ions coordinate to UDP-glucose

Corynebacterium glutamicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Magnesium	crystallization data, two magnesium ions coordinate to UDP-glucose. Magnesium is involved in catalytic mechanism	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Corynebacterium glutamicum

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Release 2023.1 (January 2023)