Information on EC 2.7.1.76 - 2'-deoxyadenosine kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.7.1.76
-
RECOMMENDED NAME
GeneOntology No.
2'-deoxyadenosine kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + 2'-deoxyadenosine = ADP + dAMP
show the reaction diagram
-
-
-
-
ATP + 2'-deoxyadenosine = ADP + dAMP
show the reaction diagram
random sequential substrate addition
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
purine deoxyribonucleosides salvage
-
Purine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:2'-deoxyadenosine 5'-phosphotransferase
2'-Deoxyguanosine can also act as acceptor. Possibly identical with EC 2.7.1.74 deoxycytidine kinase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dAdo/dCyd kinase
-
-
dAK
Q0H0H5
-
dCyd kinase/dAdo kinase I
-
-
dCyd kinase/dAdo kinase I
Lactobacillus acidophilus R-26
-
-
-
deoxycytidine kinase/deoxyadenosine kinase, dCK/dAK
P0C1F9
-
deoxycytidine kinase/deoxyadenosine kinase, dCK/dAK
Lactobacillus acidophilus R-26
P0C1F9
-
-
deoxyguanosine kinase/deoxyadenosine kinase, dGK/dAK
P0C1F9
-
deoxyguanosine kinase/deoxyadenosine kinase, dGK/dAK
Lactobacillus acidophilus R-26
P0C1F9
-
-
dGuo kinase/dAdo kinase II
-
-
dGuo kinase/dAdo kinase II
Lactobacillus acidophilus R-26
-
-
-
purine-deoxyribonucleoside kinase
-
-
-
-
kinase, deoxyadenosine (phosphorylating)
-
-
-
-
additional information
-
may be identical with EC 2.7.1.113, i.e. deoxyguanosine kinase
additional information
-
enzyme may be identical with EC 2.7.1.20, i.e. adenosine kinase
additional information
-
enzyme may be identical with EC 2.7.1.20, i.e. adenosine kinase
CAS REGISTRY NUMBER
COMMENTARY
37278-12-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gene yaaF, yaaF encodes an enzyme with specificity for deoxyadenosine and deoxycytidine
-
-
Manually annotated by BRENDA team
calf
-
-
Manually annotated by BRENDA team
newborn
-
-
Manually annotated by BRENDA team
strain R-26
SwissProt
Manually annotated by BRENDA team
Lactobacillus acidophilus R-26
strain R-26
-
-
Manually annotated by BRENDA team
Lactobacillus acidophilus R-26
strain R-26
SwissProt
Manually annotated by BRENDA team
subspecies mycoides SC
-
-
Manually annotated by BRENDA team
subspecies Mycoplasma mycoides mycoides
-
-
Manually annotated by BRENDA team
subspecies Mycoplasma mycoides mycoides strain SC
-
-
Manually annotated by BRENDA team
-
Q0H0H0
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxyadenosine
2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxycytidine
2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxyadenosine
2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
low activity
-
-
?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxycytidine
2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
low activity
-
-
?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxyadenosine
2'-deoxyadenosine-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxycytidine
2'-deoxyadenosine-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine 2'-triphosphate + 2'-deoxyadenosine
3'-deoxyadenosine-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine 2'-triphosphate + 2'-deoxycytidine
3'-deoxyadenosine-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxyadenosine
3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxycytidine
3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxyadenosine
3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxycytidine
3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
show the reaction diagram
-, Q93IG4
3% relative activity compared to deoxyadenosine
-
-
?
ATP + 1-beta-D-arabinofuranosyl cytosine
ADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
show the reaction diagram
-, Q93IG4
3% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 1-beta-D-arabinofuranosyl uracil
ADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate
show the reaction diagram
-, Q93IG4
0.3% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide
ADP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide 5'-monophosphate
show the reaction diagram
-
trivial name ribavirin
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine monophosphate
show the reaction diagram
-, Q93IG4
20% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxycytidine
ADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
show the reaction diagram
-, Q93IG4
20% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxyguanosine
ADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate
show the reaction diagram
-, Q93IG4
24% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',2'-difluoro-2'-deoxyguanosine
ADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-, Q93IG4
24% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
Q0H0H0
-
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram
Q0H0H5
enzyme activates 2',2'-difluorodesoxycytidine in vivo
-
-
?
ATP + 2',3'-dideoxyadenosine
ADP + 2',3'-dideoxyadenosine monophosphate
show the reaction diagram
-, Q93IG4
1.5% relative activity compared to deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyadenosine
ADP + 2',3'-dideoxyadenosine 5'-monophosphate
show the reaction diagram
-, Q93IG4
1.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-monophosphate
show the reaction diagram
-, Q93IG4
0.1% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyguanosine
ADP + 2',3'-dideoxyguanosine 5'-monophosphate
show the reaction diagram
-, Q93IG4
0.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyinoosine
ADP + 2',3'-dideoxyinosine 5'-monophosphate
show the reaction diagram
-, Q93IG4
3.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2',3'-dideoxyinosine
ADP + 2',3'-dideoxyinosine 5'-phosphate
show the reaction diagram
-, Q93IG4
3.9% relative activity compared to deoxyadenosine
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
ir
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-, Q54YL2
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
Q0H0H5
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
Q0H0H0
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-, Q93IG4
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
ir
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
Q0H0H5
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
Q0H0H0
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-, Q93IG4
81% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-, Q93IG4
97% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
show the reaction diagram
-, Q93IG4
116% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram
-, Q93IG4
0.01% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram
-, Q93IG4
0.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine monophosphate
show the reaction diagram
-, Q54YL2
i.e. fludarabine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine monophosphate
show the reaction diagram
-, Q93IG4
15% relative activity compared to deoxyadenosine
-
-
?
ATP + 2'-fluoro-arabinosyl adenine
ADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate
show the reaction diagram
-, Q93IG4
15% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine monophosphate
show the reaction diagram
-, Q93IG4
111% relative activity compared to deoxyadenosine
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-, Q93IG4
111% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl adenine
ADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate
show the reaction diagram
-, Q93IG4
77% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl adenine
ADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate
show the reaction diagram
-, Q93IG4
77% of the activity with 2'-deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl guanine
ADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate
show the reaction diagram
-, Q93IG4
6% relative activity compared to deoxyadenosine
-
-
?
ATP + 9-beta-D-arabinofuranosyl guanine
ADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate
show the reaction diagram
-, Q93IG4
6% of the activity with 2'-deoxyadenosine
-
-
?
ATP + adenosine
ADP + AMP
show the reaction diagram
-
-
-
?
ATP + adenosine
ADP + AMP
show the reaction diagram
-
kinetic data suggest that different catalytic sites of deoxyadenosine kinase or a different enzyme are responsible for deoxyadenosine and adenosine kinase activities
-
?
ATP + adenosine
ADP + AMP
show the reaction diagram
-, Q93IG4
7.4% relative activity compared to deoxyadenosine
-
-
?
ATP + adenosine
ADP + adenosine 5'-monophosphate
show the reaction diagram
-, Q93IG4
7.4% of the activity with 2'-deoxyadenosine
-
-
?
ATP + cytidine
ADP + CMP
show the reaction diagram
-
approx. 18% of activity with deoxyadenosine
-
?
ATP + cytidine
ADP + cytidine 5'-monophosphate
show the reaction diagram
-, Q93IG4
0.1% of the activity with 2'-deoxyadenosine
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
P0C1F9
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
Q0H0H5
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-, Q54YL2
-
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-, Q93IG4
100% activity
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
transfers a phospho group from specific nucleoside 5'-triphosphate donors to 5'-position of deoxyadenosine, no activity with adenosine and guanosine
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
no activity with cytidine, uridine, guanosine, deoxyguanosine and thymidine
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
enzyme bears two separate but interacting active sites for deoxyadenosine and deoxycytidine kinase activity
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
enzyme has both adenosine kinase and deoxyadenosine kinase activity
-
-
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
Lactobacillus acidophilus, Lactobacillus acidophilus R-26
-
enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
Lactobacillus acidophilus R-26
P0C1F9
-
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
-
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
-
involved in biosynthesis of nucleoside monophosphates from preformed deoxyribonucleosides
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
P0C1F9
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
-
involved in nucleoside metabolism
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
Lactobacillus acidophilus R-26
P0C1F9
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
Q0H0H5
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
approx. 12% of activity with deoxyadenosine
-
?
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties
-
?
ATP + deoxycytosine
ADP + dCMP
show the reaction diagram
-, Q93IG4
81% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
approx. 40% of activity with deoxyadenosine
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-, Q93IG4
97% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyinosine
ADP + dIMP
show the reaction diagram
-, Q93IG4
116% relative activity compared to deoxyadenosine
-
-
?
ATP + deoxyuridine
ADP + dUMP
show the reaction diagram
-, Q93IG4
0.9% relative activity compared to deoxyadenosine
-
-
?
ATP + fludarabine
ADP + fludarabine monophosphate
show the reaction diagram
-, Q54YL2
-
-
-
?
ATP + gemcitabine
ADP + ?
show the reaction diagram
Q0H0H5
-
-
-
?
ATP + guanosine
ADP + GMP
show the reaction diagram
-, Q93IG4
5.5% relative activity compared to deoxyadenosine
-
-
?
ATP + guanosine
ADP + guanosine 5'-monophosphate
show the reaction diagram
-, Q93IG4
5.5% of the activity with 2'-deoxyadenosine
-
-
?
ATP + inosine
ADP + IMP
show the reaction diagram
-, Q93IG4
5.9% relative activity compared to deoxyadenosine
-
-
?
ATP + inosine
ADP + inosine monophosphate
show the reaction diagram
-, Q93IG4
5.9% of the activity with 2'-deoxyadenosine
-
-
?
ATP + penciclovir
ADP + ?
show the reaction diagram
-, Q93IG4
1.4% relative activity compared to deoxyadenosine
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-, Q54YL2
-
-
-
?
CTP + 2'-deoxyadenosine
CDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-, Q54YL2
CTP, 3% of the activity with ATP
-
-
?
CTP + deoxyadenosine
CDP + dAMP
show the reaction diagram
-
-
-
?
CTP + deoxyadenosine
CDP + dAMP
show the reaction diagram
-
-
-
-
?
CTP + deoxyadenosine
CDP + dAMP
show the reaction diagram
-, Q93IG4
57% activity compared to ATP
-
-
?
dATP + deoxyadenosine
dADP + dAMP
show the reaction diagram
-, Q93IG4
2% activity compared to ATP
-
-
?
dCTP + deoxyadenosine
dCDP + dAMP
show the reaction diagram
-, Q93IG4
106% activity compared to ATP
-
-
?
dTTP + deoxyadenosine
dTDP + dAMP
show the reaction diagram
-
75% of activity with ATP
-
?
dTTP + deoxyadenosine
dTDP + dAMP
show the reaction diagram
-, Q93IG4
44% activity compared to ATP
-
-
?
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
GTP + 2'-deoxyadenosine
GDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-, Q54YL2
GTP, 14% of the activity with ATP
-
-
?
GTP + 2'-deoxycytidine
GDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
GTP + deoxyadenosine
GDP + dAMP
show the reaction diagram
-
-
-
?
triphosphate + 2'-deoxycytidine
diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
TTP + deoxyadenosine
TDP + dAMP
show the reaction diagram
-
-
-
?
TTP + deoxyadenosine
TDP + dAMP
show the reaction diagram
-
-
-
-
?
UTP + 2'-deoxyadenosine
UDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-, Q54YL2
UTP, 2% of the activity with ATP
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
-
-
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-
90% of activity with ATP
-
?
UTP + deoxyadenosine
UDP + dAMP
show the reaction diagram
-, Q93IG4
22% activity compared to ATP
-
-
?
GTP + deoxyadenosine
GDP + dAMP
show the reaction diagram
-
10% of activity with ATP
-
?
additional information
?
-
-
no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates
-
-
-
additional information
?
-
-
substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine
-
-
-
additional information
?
-
-, Q54YL2
cannot efficiently phosphorylate any other native deoxyribonucleoside than deoxyadenosine
-
-
-
additional information
?
-
-, Q93IG4
less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine
-
-
-
additional information
?
-
-, Q54YL2
enzyme cannot effectively phopshorylate any other native deoxyribonucleodie besides 2'-deoxyadenosine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
ir
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
ir
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-, Q54YL2
-
-
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
-
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
-
involved in biosynthesis of nucleoside monophosphates from preformed deoxyribonucleosides
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
P0C1F9
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
-
involved in nucleoside metabolism
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
Lactobacillus acidophilus R-26
P0C1F9
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2'-deoxyadenosine-3'-fluoro-3'-triphosphate
-
62% of the activity with ATP and deoxycytidine, 1.5% of the activity with ATP and deoxyadenosine
-
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate
-
59% of the activity with ATP and deoxycytidine, 1% of the activity with ATP and deoxyadenosine
-
2'-deoxyadenosine-3'-triphosphate
-
73% of the activity with ATP and deoxycytidine, 6% of the activity with ATP and deoxyadenosine
-
3'-deoxyadenosine-2'-triphosphate
-
97% of the activity with ATP and deoxycytidine, 60% of the activity with ATP and deoxyadenosine
-
3'-deoxyadenosine-3'-fluoro-2'-triphosphate
-
66% of the activity with ATP and deoxycytidine, 4% of the activity with ATP and deoxyadenosine
-
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate
-
62% of the activity with ATP and deoxycytidine, 4% of the activity with ATP and deoxyadenosine
-
additional information
-
triphosphate is active with 2'-deoxycytidine, 40% of the activity with ATP, not with 2'-deoxyadenosine, no activity with diphosphate as phosphate donor
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activation, can replace Mg2+
Mg2+
-
required for activity
Mg2+
-
required for activity
Mg2+
-
required for activity
Mg2+
-
required for activity
Mn2+
-
activation, can replace Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5'-amino-5'-deoxyadenosine
-
-
adenine arabinoside
-
-
CHAPS
-
at critical micelle concentration
dADP
-
0.05 mM, 70% inhibition
dAMP
-
0.2 mM, 36% inhibition
dATP
-
kinetic, mechanism, protects corresponding active site against trypsin inactivation
dATP
P0C1F9
0.5 mM, 90% inhibition of recombinant dAK, end product inhibition
dATP
-
0.05 mM, 68% inhibition
dATP
-, Q93IG4
feedback inhibitor
dCDP
-
0.001 mM, 60% inhibition
dCMP
-
0.01 mM, 88% inhibition
dCTP
-
kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
dCTP
-
0.0005 mM, 59% inhibition
dCTP
-, Q93IG4
feedback inhibitor
deoxyadenosine
-
substrate ribavirin
deoxyguanosine
-
substrate deoxyadenosine
dGDP
-
0.2 mM, 46% inhibition
dGMP
-
0.5 mM, 32% inhibition
dGTP
-
kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
dGTP
-
0.2 mM, 64% inhibition
diphosphate
-
competitive inhibition
p-chloromercuribenzoate
-
0.0007 mM, 50% inhibition of ribavirin and deoxadenosine kinase activity
ribavirin
-
deoxyadenosine as substrate
Trypsin
-
substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites
-
Hg2+
-
0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition
additional information
-
not inhibited by adenosine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2'-O-Cyclocytidine
-
activation
5-Fluoro-2'-deoxycytidine
-
0.4 mM, 275% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.028 mM
cytosine arabinoside
-
activation
Deoxycytidine
-
30 mM, 584% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.1 mM
deoxycytidine monophosphate
-
10 mM, 409% activation of deoxyadenosine phosphorylation, half-maximal activation at 1.6 mM
deoxyguanosine
P0C1F9
0.1 mM, 5fold activation of recombinant dAK
deoxyguanosine
-
5 mM, 330% activation of deoxyadenosine phosphorylation, half-maximal activation at 2.2 mM
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0335
-
2',2'-difluorodeoxycytidine
Q0H0H5
ratio kcat/Km is 5100 per s and M
0.192
-
2',2'-difluorodesoxycytidine
Q0H0H0
pH 8.0
0.0227
-
2'-deoxyadenosine
-, Q54YL2
-
0.0292
-
2'-deoxyadenosine
Q0H0H0
pH 8.0
0.0332
-
2'-deoxyadenosine
Q0H0H5
-
0.027
-
2'-deoxycytidine
Q0H0H5
-
0.0344
-
2'-deoxycytidine
Q0H0H0
pH 8.0
0.146
-
2'-fluoro-arabinosyl adenine
-, Q54YL2
-
0.007
-
ATP
-
pH 7.5, 37C, with deoxycytidine
0.011
-
ATP
-
pH 7.6, 37C, recombinant enzyme
0.016
-
ATP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.06
-
ATP
-
pH 7.5, 37C, with deoxyadenosine
0.021
-
CTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.022
-
CTP
-
pH 7.6, 37C, recombinant enzyme
0.0028
-
deoxyadenosine
-
pH 8.0, 20C, recombinant dAK
0.0049
-
deoxyadenosine
-
pH 7.4, 37C, CCRF-CEM leukemia cells
0.0075
-
deoxyadenosine
-
pH 8.0, 20C, R79K mutant dAK
0.01
-
deoxyadenosine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.012
-
deoxyadenosine
-
pH 7.6, 37C, recombinant enzyme
0.018
-
deoxyadenosine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.0227
-
deoxyadenosine
-, Q54YL2
-
0.029
-
deoxyadenosine
-
pH 7.4, 37C, erythrocyte
0.0332
-
deoxyadenosine
Q0H0H5
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.5
-
deoxyadenosine
-
pH 7.5, 37C
5
-
deoxyadenosine
-
pH 7.8, 37C
0.027
-
Deoxycytidine
Q0H0H5
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.121
-
Deoxycytidine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.146
-
Deoxycytidine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.18
-
Deoxycytidine
-
pH 7.6, 37C, recombinant enzyme
0.019
-
deoxyguanosine
-
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.02
-
deoxyguanosine
-
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.021
-
deoxyguanosine
-
pH 7.6, 37C, recombinant enzyme
0.146
-
fludarabine
-, Q54YL2
-
0.0335
-
gemcitabine
Q0H0H5
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.11
-
MgATP2-
-
pH 8.0, 20C, R79K:dGK mutant dAK
0.15
-
MgATP2-
-
pH 8.0, 20C, R79K mutant dAK
3.2
-
ribavirin
-
pH 7.5, 37C
0.004
-
thymidine
-, Q54YL2
;
0.074
-
Triphosphate
-
pH 7.5, 37C, with deoxycytidine
0.063
-
TTP
-
pH 7.6, 37C, recombinant enzyme
0.077
-
TTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.135
-
UTP
-
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.18
-
UTP
-
pH 7.6, 37C, recombinant enzyme
0.44
-
MgATP2-
-
pH 8.0, 20C, recombinant dAK
additional information
-
additional information
-
kinetic study
-
additional information
-
additional information
-
kinetic study
-
additional information
-
additional information
-
the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.19
-
2',2'-difluorodeoxycytidine
Q0H0H0
pH 8.0
1.82
-
2'-deoxyadenosine
Q0H0H0
pH 8.0
3.7
-
2'-deoxyadenosine
-, Q54YL2
-
0.56
-
2'-deoxycytidine
Q0H0H0
pH 8.0
3.3
-
2'-fluoro-arabinosyl adenine
-, Q54YL2
-
0.84
-
ATP
-
pH 7.6, 37C, recombinant enzyme
0.62
-
CTP
-
pH 7.6, 37C, recombinant enzyme
1.22
-
deoxyadenosine
Q0H0H5
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
1.48
-
deoxyadenosine
-
pH 7.6, 37C, recombinant enzyme
3.7
-
deoxyadenosine
-, Q54YL2
-
0.55
-
Deoxycytidine
Q0H0H5
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
1.23
-
Deoxycytidine
-
pH 7.6, 37C, recombinant enzyme
0.61
-
deoxyguanosine
-
pH 7.6, 37C, recombinant enzyme
3.3
-
fludarabine
-, Q54YL2
-
0.17
-
gemcitabine
Q0H0H5
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.0081
-
thymidine
-, Q54YL2
-
8.1
-
thymidine
-, Q54YL2
-
1.64
-
TTP
-
pH 7.6, 37C, recombinant enzyme
0.36
-
UTP
-
pH 7.6, 37C, recombinant enzyme
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.035
-
adenosine
-
pH 7.5, 37C, substrate deoxyadenosine
0.057
-
adenosine
-
pH 7.5, 37C, substrate ribavirin
0.001
-
dATP
-
pH 8.0, 20C, competitive vs. ATP
0.0014
-
dATP
-
pH 8.0, 20C, noncompetitive vs. deoxadenosine
0.97
-
deoxyadenosine
-
pH 7.5, 37C, substrate ribavirin
0.08
-
diphosphate
-
pH 7.5, 37C, with deoxyadenosine, versus ATP
0.31
-
diphosphate
-
pH 7.5, 37C, with deoxycytidine, versus ATP or triphosphate
2.5
-
ribavirin
-
pH 7.5, 37C, substrate deoxyadenosine
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0000045
-
-
in 30 mM HEPES-NaOH buffer (pH 7.6), 10 mM MgCl2, 1 mM DTT, 3.75 mM ATP and 0.055 mM deoxyadenosine
0.007
-
-
purified recombinant enzyme, substrates deoxycytidine and ATP
0.027
-
-
substrate 2'-deoxycytidine and ATP
0.06
-
-
substrate ribavarin
0.073
-
-
-
0.43
-
-
purified recombinant enzyme, substrates deoxyadenosine and ATP
0.54
-
-
substrate 2'-deoxyadenosine and ATP
2.3
-
-
dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine
additional information
-
-
0.00006 mmol/ml min
additional information
-
-
activity increases 5.4fold between days 7 and 9 of mouse embryonic development
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-
with deoxyadenosine and ribavirin as substrates
7.5
-
-
broad optimum, 10% difference in activity between pH 6.5 and pH 8.5
7.5
-
-
assay at
8.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.7
8.8
-
approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8
6
9
-
approx. 30% of maximal activity at pH 6.0 and pH 9.0, respectively
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
44
-
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
27
50
-
approx. half-maximal activity at 27C and 50C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
low activity
Manually annotated by BRENDA team
-
very low activity
Manually annotated by BRENDA team
-
peripheral from adult, very low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
peripheral from adult
Manually annotated by BRENDA team
-
suspension culture
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
26200
-
Q0H0H5
SDS-PAGE
50000
-
-
gel filtration
50000
-
-, Q93IG4
Superdex 75 column gel filtration
63000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-, Q54YL2
x * 28600, calculated
?
Q0H0H0
x * 26400, calculated
dimer
-
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
dimer
-
2 * 27000, His-tagged enzyme, SDS-PAGE
dimer
-, Q93IG4
2 * 25000, Superdex 75 column gel filtration
dimer
Lactobacillus acidophilus R-26
-
1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
-
additional information
-
both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits
additional information
P0C1F9
dGK/dAK is composed of 2 nonidentical subunits of approx. 26000 Da
additional information
Lactobacillus acidophilus R-26
-
both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits; dGK/dAK is composed of 2 nonidentical subunits of approx. 26000 Da
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
three-dimensional structural model based on crystal structure of Mycoplasma mycoides deoxyadenosine kinase
-, Q54YL2
in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site; sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCN
-, Q93IG4
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
-
half-life of deoxyadenosine kinase activity: 1.5 days
37
-
-
t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize
42
-
-
first-order rate constant of inactivation: in the absence of dithiothreitol, 14.2/min, in the presence of 10 mM dithiothreitol, 6.82, substrate ribavirin
48
-
-
inactivation of deoxyadenosine kinase activity
50
-
-
80% loss of activity
additional information
-
-
temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
additional information
-
-
dithiothreitol protects against heat inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
glycerol, 50%, or dithiothreitol stabilizes
-
stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
-
dATP stabilizes
-
dithiothreitol protects against heat inactivation, glycerol and dithiothreitol increase stability during storage
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 60% glycerol, at least 3 months, slight loss of activity
-
4C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin
-
ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4C
-
dATP stabilizes during storage
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
glutathione Sepharose FF column chromatography; recombinant protein
Q0H0H5
affinity chromatography on Cibacron Blue F3G-A
-
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150
-
affinity chromatography
-
recombinant dGK/dAK, dATP-Sepharose affinity chromatography
P0C1F9
recombinant His-tagged enzyme, metal affinity chromatography
-
Superdex 75 column gel filtration
-, Q93IG4
ammonium sulfate, Sephadex G-100, DEAE-cellulose, Sephadex G-75
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli; expression in Escherichia coli
Q0H0H5
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli
-
expression in Escherichia coli
-, Q54YL2
expression of dGK/dAK in Escherichia coli
P0C1F9
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D78A
-
mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity
D78E
-
mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity
D78N
-
mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity
D84A
-
mutation in dGK of dAK/dGK, increase of dAK activity
D84E
-
mutation in dGK of dAK/dGK, increase of dAK activity
D84N
-
mutation in dGK of dAK/dGK, increase of dAK activity
R79k
-
2 types of mutants, one bears the mutation on both the dAK and dGK subunits while the other, called R79K:dGK, has the mutation on the dGK subunit only, strong increase in dAK activity, activation of dAK by deoxyguanosine is nearly eliminated in the case of R79K:dGK, while for the tandem mutated R79K mutant a 30% inhibition is observed, inhibition by dATP is reduced
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
thymocytes lacking adenosine deaminase activity accumulate intracellular dATP and undergo apoptosis. Inhibition of both deoxycytidine kinase and adenosine kinase completely abrogates accumulation of dATP and significantly reduces the induction of apoptosis