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Literature summary for 2.7.1.76 extracted from
- Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinases (2003), Biochem. Biophys. Res. Commun., 301, 192-197.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | competitive inhibition | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity | Homo sapiens | |
| 0.007 | - |
ATP | pH 7.5, 37°C, with deoxycytidine | Homo sapiens | |
| 0.06 | - |
ATP | pH 7.5, 37°C, with deoxyadenosine | Homo sapiens | |
| 0.074 | - |
Triphosphate | pH 7.5, 37°C, with deoxycytidine | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyadenosine | Homo sapiens | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
ir | |
| ATP + 2'-deoxycytidine | Homo sapiens | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.007 | - |
purified recombinant enzyme, substrates deoxycytidine and ATP | Homo sapiens |
| 0.43 | - |
purified recombinant enzyme, substrates deoxyadenosine and ATP | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
ir | |
| ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
ir | |
| additional information | no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates | Homo sapiens | ? | - |
? | |
| triphosphate + 2'-deoxycytidine | - |
Homo sapiens | diphosphate + 2'-deoxycytidine 5'-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dCK | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| additional information | triphosphate is active with 2'-deoxycytidine, 40% of the activity with ATP, not with 2'-deoxyadenosine, no activity with diphosphate as phosphate donor | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
diphosphate | pH 7.5, 37°C, with deoxyadenosine, versus ATP | Homo sapiens | |
| 0.31 | - |
diphosphate | pH 7.5, 37°C, with deoxycytidine, versus ATP or triphosphate | Homo sapiens | |
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