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Literature summary for 2.7.1.76 extracted from

  • Sandrini, M.P.B.; Soederbom, F.; Mikkelsen, N.E.; Piskur, J.
    Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases (2007), J. Mol. Biol., 369, 653-664.
    View publication on PubMed
Search for more literature for 2.7.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Dictyostelium discoideum

Crystallization (Commentary)

Crystallization (Comment) Organism
three-dimensional structural model based on crystal structure of Mycoplasma mycoides deoxyadenosine kinase Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 thymidine
-
 Dictyostelium discoideum
0.0227
-
 2'-deoxyadenosine
-
 Dictyostelium discoideum
0.0227
-
 deoxyadenosine
-
 Dictyostelium discoideum
0.146
-
 fludarabine
-
 Dictyostelium discoideum
0.146
-
 2'-fluoro-arabinosyl adenine
-
 Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28600
-
 x * 28600, calculated Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxyadenosine Dictyostelium discoideum
-
 ADP + dAMP
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-
Dictyostelium discoideum Q54YL2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyadenosine
-
 Dictyostelium discoideum ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-fluoro-arabinosyl adenine i.e. fludarabine Dictyostelium discoideum ADP + 2'-fluoro-arabinosyl adenine monophosphate
-
 ?
ATP + deoxyadenosine
-
 Dictyostelium discoideum ADP + dAMP
-
 ?
ATP + fludarabine
-
 Dictyostelium discoideum ADP + fludarabine monophosphate
-
 ?
ATP + thymidine
-
 Dictyostelium discoideum ADP + thymidine 5'-phosphate
-
 ?
CTP + 2'-deoxyadenosine CTP, 3% of the activity with ATP Dictyostelium discoideum CDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
GTP + 2'-deoxyadenosine GTP, 14% of the activity with ATP Dictyostelium discoideum GDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
additional information cannot efficiently phosphorylate any other native deoxyribonucleoside than deoxyadenosine Dictyostelium discoideum ?
-
 ?
additional information enzyme cannot effectively phopshorylate any other native deoxyribonucleodie besides 2'-deoxyadenosine Dictyostelium discoideum ?
-
 ?
UTP + 2'-deoxyadenosine UTP, 2% of the activity with ATP Dictyostelium discoideum UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 28600, calculated Dictyostelium discoideum

Synonyms

Synonyms Comment Organism
dAK
-
 Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0081
-
 thymidine
-
 Dictyostelium discoideum
3.3
-
 fludarabine
-
 Dictyostelium discoideum
3.3
-
 2'-fluoro-arabinosyl adenine
-
 Dictyostelium discoideum
3.7
-
 2'-deoxyadenosine
-
 Dictyostelium discoideum
3.7
-
 deoxyadenosine
-
 Dictyostelium discoideum
8.1
-
 thymidine
-
 Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum