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Literature summary for 2.7.1.76 extracted from

  • Streeter, D.G.; Simon, L.N.; Robins, R.K.; Miller, J.P.
    The phosphorylation of ribavirin by deoxyadenosine kinase from rat liver. Differentiation between adenosine and deoxyadenosine kinase (1974), Biochemistry, 13, 4543-4549.
    View publication on PubMed
Search for more literature for 2.7.1.76

General Stability

General Stability Organism
dithiothreitol protects against heat inactivation, glycerol and dithiothreitol increase stability during storage Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
adenosine
-
 Rattus norvegicus
deoxyadenosine substrate ribavirin Rattus norvegicus
p-chloromercuribenzoate 0.0007 mM, 50% inhibition of ribavirin and deoxadenosine kinase activity Rattus norvegicus
ribavirin deoxyadenosine as substrate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 deoxyadenosine pH 7.5, 37°C Rattus norvegicus
3.2
-
 ribavirin pH 7.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-100, DEAE-cellulose, Sephadex G-75 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 substrate ribavarin Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide trivial name ribavirin Rattus norvegicus ADP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide 5'-monophosphate
-
 ?
ATP + adenosine kinetic data suggest that different catalytic sites of deoxyadenosine kinase or a different enzyme are responsible for deoxyadenosine and adenosine kinase activities Rattus norvegicus ADP + AMP
-
 ?
ATP + deoxyadenosine no activity with cytidine, uridine, guanosine, deoxyguanosine and thymidine Rattus norvegicus ADP + dAMP
-
 ?
ATP + ribavirin
-
 Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 dithiothreitol protects against heat inactivation Rattus norvegicus
42
-
 first-order rate constant of inactivation: in the absence of dithiothreitol, 14.2/min, in the presence of 10 mM dithiothreitol, 6.82, substrate ribavirin Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 with deoxyadenosine and ribavirin as substrates Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 approx. 30% of maximal activity at pH 6.0 and pH 9.0, respectively Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
 adenosine pH 7.5, 37°C, substrate deoxyadenosine Rattus norvegicus
0.057
-
 adenosine pH 7.5, 37°C, substrate ribavirin Rattus norvegicus
0.97
-
 deoxyadenosine pH 7.5, 37°C, substrate ribavirin Rattus norvegicus
2.5
-
 ribavirin pH 7.5, 37°C, substrate deoxyadenosine Rattus norvegicus