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Literature summary for 2.7.1.76 extracted from

  • Hong, Y.S.; Ma, G.T.; Ives, D.H.
    Directed mutagenesis of deoxyguanosine site at arginine 79 up-regulates turnover on deoxyadenosine kinase subunit of heterodimeric enzyme from Lactobacillus acidophilus R26 (1995), J. Biol. Chem., 270, 6602-6606.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D78A mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity Lactobacillus acidophilus
D78E mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity Lactobacillus acidophilus
D78N mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity Lactobacillus acidophilus
R79k 2 types of mutants, one bears the mutation on both the dAK and dGK subunits while the other, called R79K:dGK, has the mutation on the dGK subunit only, strong increase in dAK activity, activation of dAK by deoxyguanosine is nearly eliminated in the case of R79K:dGK, while for the tandem mutated R79K mutant a 30% inhibition is observed, inhibition by dATP is reduced Lactobacillus acidophilus

Inhibitors

Inhibitors Comment Organism Structure
dATP
-
Lactobacillus acidophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
deoxyadenosine pH 8.0, 20°C, recombinant dAK Lactobacillus acidophilus
0.0075
-
deoxyadenosine pH 8.0, 20°C, R79K mutant dAK Lactobacillus acidophilus
0.11
-
ATP pH 8.0, 20°C, R79K:dGK mutant dAK Lactobacillus acidophilus
0.11
-
ATP ATP in form of MgATP2- Lactobacillus acidophilus
0.15
-
ATP pH 8.0, 20°C, R79K mutant dAK Lactobacillus acidophilus
0.15
-
ATP ATP in form of MgATP2- Lactobacillus acidophilus
0.44
-
ATP pH 8.0, 20°C, recombinant dAK Lactobacillus acidophilus
0.44
-
ATP ATP in form of MgATP2- Lactobacillus acidophilus

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus P0C1F9
-
-
Lactobacillus acidophilus ATCC 700396 P0C1F9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyadenosine
-
Lactobacillus acidophilus ADP + dAMP
-
?
ATP + 2'-deoxyadenosine
-
Lactobacillus acidophilus ATCC 700396 ADP + dAMP
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
dATP pH 8.0, 20°C, competitive vs. ATP Lactobacillus acidophilus
0.0014
-
dATP pH 8.0, 20°C, noncompetitive vs. deoxadenosine Lactobacillus acidophilus