Protein Variants | Comment | Organism |
---|---|---|
D78A | mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity | Lactobacillus acidophilus |
D78E | mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity | Lactobacillus acidophilus |
D78N | mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity | Lactobacillus acidophilus |
R79k | 2 types of mutants, one bears the mutation on both the dAK and dGK subunits while the other, called R79K:dGK, has the mutation on the dGK subunit only, strong increase in dAK activity, activation of dAK by deoxyguanosine is nearly eliminated in the case of R79K:dGK, while for the tandem mutated R79K mutant a 30% inhibition is observed, inhibition by dATP is reduced | Lactobacillus acidophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dATP | - |
Lactobacillus acidophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
deoxyadenosine | pH 8.0, 20°C, recombinant dAK | Lactobacillus acidophilus | |
0.0075 | - |
deoxyadenosine | pH 8.0, 20°C, R79K mutant dAK | Lactobacillus acidophilus | |
0.11 | - |
ATP | pH 8.0, 20°C, R79K:dGK mutant dAK | Lactobacillus acidophilus | |
0.11 | - |
ATP | ATP in form of MgATP2- | Lactobacillus acidophilus | |
0.15 | - |
ATP | pH 8.0, 20°C, R79K mutant dAK | Lactobacillus acidophilus | |
0.15 | - |
ATP | ATP in form of MgATP2- | Lactobacillus acidophilus | |
0.44 | - |
ATP | pH 8.0, 20°C, recombinant dAK | Lactobacillus acidophilus | |
0.44 | - |
ATP | ATP in form of MgATP2- | Lactobacillus acidophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus acidophilus | P0C1F9 | - |
- |
Lactobacillus acidophilus ATCC 700396 | P0C1F9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | - |
Lactobacillus acidophilus | ADP + dAMP | - |
? | |
ATP + 2'-deoxyadenosine | - |
Lactobacillus acidophilus ATCC 700396 | ADP + dAMP | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
dATP | pH 8.0, 20°C, competitive vs. ATP | Lactobacillus acidophilus | |
0.0014 | - |
dATP | pH 8.0, 20°C, noncompetitive vs. deoxadenosine | Lactobacillus acidophilus |