Information on EC 2.7.1.23 - NAD+ kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.23
-
RECOMMENDED NAME
GeneOntology No.
NAD+ kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + NAD+ = ADP + NADP+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
NAD metabolism
-
-
NAD phosphorylation and dephosphorylation
-
-
NAD phosphorylation and transhydrogenation
-
-
NAD/NADH phosphorylation and dephosphorylation
-
-
NAD/NADP-NADH/NADPH cytosolic interconversion (yeast)
-
-
NAD/NADP-NADH/NADPH mitochondrial interconversion (yeast)
-
-
Nicotinate and nicotinamide metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:NAD+ 2'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9032-66-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Achromobacter aceris
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. lactofermentum
-
-
Manually annotated by BRENDA team
Corynebacterium glutamicum subsp. lactofermentum
-
-
-
Manually annotated by BRENDA team
Corynebacterium glutamicum subsp. lactofermentum JHI3-156
-
-
-
Manually annotated by BRENDA team
Entamoeba histolytica HM1: IMSS cl 6
-
UniProt
Manually annotated by BRENDA team
strain Z
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ATCC 1325
-
-
Manually annotated by BRENDA team
Sorghum sp.
-
-
-
Manually annotated by BRENDA team
expression in Escherichia coli
Swissprot
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
the reduction of the NADP(H) level in the sll1415 mutant leads to a significant accumulation of glucose-6-phosphate and a loss of photoheterotrophic growth
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + NAD+
AMP + NADP+
show the reaction diagram
ADP + NADH
AMP + NADPH
show the reaction diagram
13% activity compared to ATP
-
-
?
ATP + 3-acetyl pyridineadenine dinucleotide
?
show the reaction diagram
-
-
-
-
-
ATP + NAD+
ADP + NADP+
show the reaction diagram
ATP + NADH
ADP + NADPH
show the reaction diagram
CTP + NAD+
CDP + NADP+
show the reaction diagram
CTP + NADH
CDP + NADPH
show the reaction diagram
dATP + NAD+
dADP + NADP+
show the reaction diagram
dATP + NADH
dADP + NADPH
show the reaction diagram
103% activity compared to ATP
-
-
?
dCTP + NAD+
dCDP + NADP+
show the reaction diagram
dGTP + NAD+
dGDP + NADP+
show the reaction diagram
di-adenosine diphosphate
?
show the reaction diagram
-
-
-
-
?
diphosphate + NAD+
phosphate + NADP+
show the reaction diagram
-
10% of the activity with ATP
-
-
?
dITP + NAD+
dIDP + NADP+
show the reaction diagram
-
141% activity compared to ATP
-
-
?
dTTP + NAD+
dTDP + NADP+
show the reaction diagram
-
35% of the activity with ATP
-
-
?
fructose-1,6-bisphosphate + ?
?
show the reaction diagram
-
-
-
?
GTP + NAD+
GDP + NADP+
show the reaction diagram
GTP + NADH
GDP + NADPH
show the reaction diagram
102% activity compared to ATP
-
-
?
hexametaphosphate + NAD+
? + NADP+
show the reaction diagram
-
194% activity compared to ATP
-
-
?
hexametaphosphate + NAD+
NADP+ + ?
show the reaction diagram
-
47% of the activity with ATP
-
-
?
hexaphosphate + NAD+
pentaphosphate + NADP+
show the reaction diagram
hexapolyphosphate + NAD+
pentapolyphosphate + NADP+
show the reaction diagram
ITP + NAD+
IDP + NADP+
show the reaction diagram
metaphosphate + NAD+
? + NADP+
show the reaction diagram
-
280% activity compared to ATP
-
-
?
NAD+ + ATP
NADP+ + ADP
show the reaction diagram
NAD+ + poly(P)4
NADP+ + poly(P)3
show the reaction diagram
-
-
-
-
?
NADH + ATP
NADPH + ADP
show the reaction diagram
-
-
-
-
?
NADH + poly(P)4
NADPH + poly(P)3
show the reaction diagram
polyphosphate + NAD+
(phosphate)n-1 + NADP+
show the reaction diagram
polyphosphate + NAD+
?
show the reaction diagram
polyphosphate(20) + NAD+
polyphosphate(19) + NADP+
show the reaction diagram
polyphosphate(25) + NAD+
polyphosphate(24) + NADP+
show the reaction diagram
polyphosphate(45) + NAD+
polyphosphate(44) + NADP+
show the reaction diagram
polyphosphate(65) + NAD+
polyphosphate(64) + NADP+
show the reaction diagram
-
-
-
-
?
polyphosphate(75) + NAD+
polyphosphate(74) + NADP+
show the reaction diagram
-
-
-
-
?
tetraphosphate + NAD+
triphosphate + NADP+
show the reaction diagram
-
-
-
-
?
tetrapolyphosphate + NAD+
tripolyphosphate + NADP+
show the reaction diagram
-
84% activity compared to ATP
-
-
?
triphosphate + NAD+
diphosphate + NADP+
show the reaction diagram
-
-
-
-
?
TTP + NAD+
TDP + NADP+
show the reaction diagram
TTP + NADH
TDP + NADPH
show the reaction diagram
50% activity compared to ATP
-
-
?
UTP + NAD+
UDP + NADP+
show the reaction diagram
UTP + NADH
UDP + NADPH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + NAD+
ADP + NADP+
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CoCl2
31% of activity with MgCl2
CuCl2
-
5 mM, substrate polyP27, 100% activity, substrate ATP, 62% activity
Ni2+
6% activity at 5 mM compared to Mg2+
NiCl2
-
5 mM, substrate polyP27, 80% activity, substrate ATP, 36% activity
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S,3R)-5-hydroxy-6,8,10-trimethoxy-2,3-dimethyl-2,3-dihydro-4H-naphtho-(2,3-b)-pyran-4-one
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0243 mM
(2S,3R)-5-hydroxy-6,8-dimethoxy-2,3-dimethyl-2,3-dihydro-4H-naphtho-(2,3-b)-pyran-4-one
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0422 mM
(2S,3S)-5-hydroxy-6,8,10-trimethoxy-2,3-dimethyl-4H-2,3-dihydronaphtho-(2,3-b)-pyran-4-one
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.022 mM
(2S,3S)-5-hydroxy-6,8-dimethoxy-2,3-dimethyl-4H-2,3-dihydronaphtho-(2,3-b)-pyran-4-one
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0401 mM
2'-AMP
-
2 mM, 25% inhibition
2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide
-
3',5'-cAMP
-
3.5 mM, 20% inhibition
3'-AMP
-
2 mM, 20% inhibition
3-(bromoacetyl)pyridine
-
-
3-acetyl-pyridine adenine dinucleotide
-
1 mM, 30% inhibition
3-acetylpyridine-adenine-dinucleotide (oxidized form)
-
0.5 mM, 10% inhibition
3-aldehyde-pyridine adenine dinucleotide
-
1 mM, 32% inhibition
5'-AMP
-
2 mM, 15% inhibition
5'-thioacetyladenosine
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
0.5 mM, 62% inhibition of isoenzyme 1 and 33% inhibition of isoenzyme 2
5-hydroxy-6,8-dimethoxy-2,3-dimethyl-4H-naphtho-(2,3-b)-pyran-4-one
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0171 mM
adenosine diphosphoribose
-
3.5 mM, 31% inhibition
AMP
-
3.5 mM, 29% inhibition
CH3HgBr
-
0.1 mM, 63% inhibition
chlorpromazin
-
5 mM, 11% inhibition of the 410000 Da isoform, 80% inhibition of the 63000 Da isoform
citrate
-
3.5 mM, 55% inhibition
citrinin
-
phytotoxin from Guanomyces polythrix, 50% inhibition above 0.120 mM
deaminopyridinealdehyde adenine dinucleotide
-
0.5 mM, 10% inhibition
di-(5'-thioadenosine)
-
-
diphosphate
-
5 mM, 46% inhibition of the 410000 Da isoform, 69% inhibition of the 63000 Da isoform
dithiothreitol
-
0.1 mM, 81% inhibition, irreversible for isoenzyme 1 and reversible for isoenzyme 2
DTNB
-
0.1 mM, 74% inhibition
EGTA
-
5 mM, 20% inhibition of the 410000 Da isoform, 85% inhibition of the 63000 Da isoform
ergosta-4,6,8(14),22-tetraen-3-one
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.090 mM
Hg(CH3COO)2
-
0.1 mM, complete inhibition
iodoacetic acid
-
1 mM, 56% inhibition of the 63000 Da isoform, no inhibition of the 410000 Da isoform
KCl
-
200 mM KCl decreases the polyphosphate-dependent activity to 75%
NaCl
-
200 mM NaCl decreases the polyphosphate-dependent activity to 75%
NAD+
-
inhibits exchange reaction between ADP and ATP
NEM
-
1 mM, 92% inhibition of the 63000 Da isoform, 34% inhibition of the 410000 Da isoform
Ni2+
-
ATP-dependent activity is reduced to 60% or 7% in the presence of 5 or 20 mM Ni2+
nicotinamide guanine dinucleotide
-
1 mM, 23% inhibition
nicotinamide hypoxanthine dinucleotide
-
1 mM, 24% inhibition
nicotinamide mononucleotide
-
2 mM, 15% inhibition
nicotinic acid adenine dinucleotide phosphate
-
1 mM, 37% inhibition
phosphoenolpyruvate
-
3.5 mM, 20% inhibition
pyruvate
-
3.5 mM, 10% inhibition
R24571
-
1 mM, 46% inhibition of the 410000 Da isoform, 89% inhibition of the 63000 da isoform
rubrofusarin B
-
phytotoxin from Guanomyces polythrix, 50% inhibition at 0.0133 mM
shRNA
-
shRNA1-NADK exhibits a notably higher efficiency compared with a shRNA2-NADK construct. About 70% decrease of both NADK expression, activity, and the NADPH concentration, accompanied by increased sensitivity toward H2O2
-
Trifluoperazine
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-AMP
-
1.56fold activation at 3.0 mM
adenine
-
5 mM, activity of both isoenzymes is modestly enhanced
AMP
-
5 mM, activity of both isoenzymes is modestly enhanced
Caffeine
-
5 mM, activity of both isoenzymes is modestly enhanced
Calmodulin
D-ribose 5-phosphate
-
5 mM, activity of both isoenzymes is modestly enhanced
H2O2
upregulates NADK1 by 3fold after treatment of plant cells with 5 mM
imidazole
-
5 mM, activity of both isoenzymes is modestly enhanced
nicotinic acid
-
5 mM, activity of both isoenzymes is modestly enhanced
Pyridine
-
5 mM, activity of both isoenzymes is modestly enhanced
quinolinic acid
-
allosteric activator
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.3
ADP
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
0.032 - 4.5
ATP
0.59
CTP
-
pH 8
0.43
fructose-1,6-bisphosphate
-
2.86
GTP
-
pH 8
1.54
ITP
-
pH 8
0.08 - 0.74
MgATP2-
0.2
MgGTP2-
-
isoenzyme 2
0.11 - 6.5
NAD+
0.05 - 2
NADH
0.21 - 0.33
poly(P)4
2.2 - 2.7
Polyphosphate
5.9
polyphosphate(20)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
17.2
polyphosphate(25)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
14.1
polyphosphate(45)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
-
18.4
polyphosphate(65)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
-
3.1
polyphosphate(75)
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
-
2.1
Tetraphosphate
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
12
Tetrapolyphosphate
-
in 100 mM Tris-HCl (pH 8.0), at 37C
2.4
Triphosphate
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
0.42
UTP
-
pH 8
additional information
additional information
-
allosteric enzyme, Hill coefficient for ATP 1.5, for polyphosphate 1.4
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.3
ADP
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
0.52 - 399
ATP
212
fructose-1,6-bisphosphate
Methanocaldococcus jannaschii
Q58327
-
0.41 - 424
NAD+
1.9
NADH
Entamoeba histolytica
C4LSN1
in 100 mM Tris-HCl (pH 7.5), at 37C
212
NADP+
Methanocaldococcus jannaschii
Q58327
-
236
NADPH
Methanocaldococcus jannaschii
Q58327
-
7.8
polyphosphate(20)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
7.3
polyphosphate(25)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
3.4
polyphosphate(45), polyphosphate(65)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
-
1.8
polyphosphate(75)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
-
8.7
Tetraphosphate
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
3
Triphosphate
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
additional information
additional information
Columba sp.
-
the molecular activity is 360 per min, the activity per active centre is less than 60 per min, pH 7.4, 30C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
ADP
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
13
0.24 - 59.6
ATP
0.36 - 40.5
NAD+
56.48
NADH
Entamoeba histolytica
C4LSN1
in 100 mM Tris-HCl (pH 7.5), at 37C
8
1.3
polyphosphate(20)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
42445
0.42
polyphosphate(25)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
42446
0.24
polyphosphate(45)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
42447
0.19
polyphosphate(65)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
42448
0.58
polyphosphate(75)
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
42449
4.1
Tetraphosphate
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
6599
1.2
Triphosphate
Corynebacterium glutamicum
-
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30C
940
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
2-([6-amino-9-[(2R,3R,4S,5R)-5-(aminomethyl)-3,4-dihydroxytetrahydrofuran-2-yl]-9H-purin-8-yl]sulfanyl)-N-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]acetamide
in 50 mM Tris-HCl, pH 7.4, 10 mM MgCl2, at 30C
3.6
5'-thioacetyladenosine
-
-
0.02
di-(5'-thioadenosine)
-
-
0.03 - 1.02
NADH
0.13 - 0.43
NADP+
0.013 - 0.22
NADPH
2.8
Trifluoperazine
-
pH 8
0.01
W-7
-
pH 8
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0009966
-
-
0.281
30C, pH 8.0
0.5
-
substrates ATP, NAD+, mutant G208A, 37C, pH 8.0
0.7
-
substrates ATP, NAD+, mutant S199A, 37C, pH 8.0
1.1
-
substrates ATP, NAD+, mutant T195A, 37C, pH 8.0
1.2
-
substrates ATP, NAD+, wild-type, 37C, pH 8.0
1.4
-
pH 7.0, 37C, substrate NAD+, cosubstrate poly(P)4, mutant G183R
1.78
-
pH 7.0, 37C, substrate NAD+, cosubstrate ATP, mutant G183R
2.2
-
substrates polyphosphate, NAD+, mutant G208A, 37C, pH 8.0
2.33
-
pH 7.0, 37C, mutant R175G, substrate NAD+
2.5
-
substrates polyphosphate, NAD+, mutant S199A, 37C, pH 8.0
2.68
-
pH 7.0, 37C, mutant R175, substrate ATP
3.5
-
substrates polyphosphate, NAD+, mutant T195A, 37C, pH 8.0
3.8
-
substrates polyphosphate, NAD+, wild-type, 37C, pH 8.0
3.84
-
activated by Ca2+-calmodulin
8.63
-
pH 7.0, 37C, substrate NAD+, cosubstrate poly(P)4, wild-type
11.1
-
isoform NADK1, pH 7.9, 25C; pH 7.9, 25C
12.45
-
pH 7.0, 37C, wild-type, substrate ATP
12.86
-
pH 7.0, 37C, wild-type, substrate NAD+
14.3
-
isoform NADK2, pH 7.9, 25C; pH 7.9, 25C
18.67
-
pH 7.0, 37C, substrate NAD+, cosubstrate ATP, wild-type
23.2
-
ATP-dependent activity
24.3
-
polyphosphate-dependent activity
213
-
; calmodulin-depndent isoform, pH 7.9, 25C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.6
-
enzyme from liver
7 - 9.5
-
-
7.1
-
for polyphosphate-dependent activity
7.2 - 8
7.3
-
for ATP-dependent activity
7.4 - 7.5
-
-
7.5 - 8
-
410000 Da Ca2+-calmodulin-independent isoform and 63000 Da Ca2+-calmodulin-dependent isoform
7.9
-
; isoforms NADK1, NADK2
9
-
glycine-NaOH buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
-
about 50% of maximal activity at pH 5.5 and pH 7.5
6 - 10
-
pH 6.0: about 40% of maximal activity, pH 10.0: about 80% of maximal activity
6 - 7
-
activity decreases sharply below pH 6.0 and above pH 7.0
6.5 - 7.2
-
90% of maximal activity at pH 6.5 and pH 7.2
6.5 - 9.5
-
pH 6.5: about 35% of maximal activity, pH 9.5: about 90% of maximal activity
6.8 - 9.2
-
90% of maximal activity between pH 6.8 and pH 9.2, 410000 Da Ca2+-calmodulin-independent isoform
7 - 10
-
pH 7.0: about 35% of maximal activity, pH 10.0: about 50% of maximal activity
7.2 - 8.5
-
90% of maximal activity between pH 7.2 and pH 8.5, 63000 Da Ca2+-calmodulin-dependent isoform
additional information
-
broad
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
-
25C: about 45% of maximal activity, 50C: about 70% of maximal activity
37 - 68
-
about 50% of maximal activity at 37C and at 68C
80 - 100
-
80C: about 45% of maximal activity, 100C: optimum
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9
-
isoelectric focusing
6.5
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
maximal activity is observed on day 5 in light-exposed calli or day 14 in day-exposed ones. After 5 d of salt exposure, NAD kinase activity is markedly lower than in control, but NAD kinase of dark-exposed and NaCl-exposed calli is 10times more active than that of both light-exposed and NaCl-exposed calli
Manually annotated by BRENDA team
-
initiated from white and friable root calli
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
isoform NADK1
Manually annotated by BRENDA team
additional information
-
both isoforms NADK1 and NADK2 are present in all tissues examined and throughout development
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
-
gel filtration
63000
-
Ca2+-calmodulin-dependent isoenzyme
64000
gel filtration
68000
-
gel filtration
75000
-
gel filtration
82000
-
gel filtration
124000
-
equilibrium sedimentation
140000
145000
-
gel filtration
167500
-
gel filtration
169000
-
gel filtration
172000
-
gel filtration
180000
-
gel filtration
200000
260000
-
non-denaturing PAGE
270000
-
gel filtration
410000
-
Ca2+-calmodulin-independent NAD+ kinase, gel filtration
480000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
homodimer
homohexamer
homooctamer
6 * 60000, Yef1p; 8 * 60000
homotetramer
octamer
oligomer
-
x * 32000, SDS-PAGE
tetramer
trimer
-
or dimer, 3 * 33000, SDS-PAGE
additional information
-
Ca2+-dependent calmodulin-binding domain near N-terminal extension of isoform NADK2