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Literature summary for 2.7.1.23 extracted from
- NAD kinase levels control the NADPH concentration in human cells (2007), J. Biol. Chem., 282, 33562-33571.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | overexpression of NADK results in an almost 200fold increase of both the mRNA level, the protein amount and the catalytic activity | Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | key role of NADK in animal cells for the maintenance of the cellular NADPH pool. NADK overexpression and the ensuing increase of the NADPH level only moderately enhances protection against oxidant treatment | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in HEK-293 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | may become inhibitory at very high concentrations (mM) | Homo sapiens |  |
| shRNA | shRNA1-NADK exhibits a notably higher efficiency compared with a shRNA2-NADK construct. About 70% decrease of both NADK expression, activity, and the NADPH concentration, accompanied by increased sensitivity toward H2O2 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293 cell | - |
Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NAD+ | preferentially accepts NAD+ as substrate. At 100 micromol, NAD+ is 10fold faster phosphorylated than NADH | Homo sapiens | ADP + NADP+ | - |
? | |
| ATP + NADH | conversion is slow at a NADH concentration of 5 mM. At lower concentrations (50-150 micromol) and particularly when NAD+ is concomitantly present as substrate, NADH becomes farmore efficiently phosphorylated | Homo sapiens | ADP + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADK | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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