Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | independent on calmodulin | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 0.5 mM, 62% inhibition of isoenzyme 1 and 33% inhibition of isoenzyme 2 | Euglena gracilis | |
dithiothreitol | 0.1 mM, 81% inhibition, irreversible for isoenzyme 1 and reversible for isoenzyme 2 | Euglena gracilis | |
additional information | no inhibition by 2-mercaptoethanol, isoenzyme 1 and 2 | Euglena gracilis | |
PCMB | 0.5 mM, 74% inhibition of isoenzyme 1, 23% inhibition of isoenzyme 2 | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
ATP | isoenzyme 1 | Euglena gracilis | |
0.2 | - |
GTP | isoenzyme 2 | Euglena gracilis | |
0.2 | - |
GTP | GTP in form of MgGTP2- | Euglena gracilis | |
0.26 | - |
NAD+ | isoenzyme 1 | Euglena gracilis | |
1.03 | - |
NAD+ | isoenzyme 2 | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | isoenzyme 1 and 2 | Euglena gracilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Euglena gracilis | - |
isoenzyme 1 and 2 | - |
Euglena gracilis Z | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + NAD+ = ADP + NADP+ | isoenzyme 1: ping-pong mechanism. Isoenzyme 2: sequential mechanism | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | isoenzyme 1: most effective phosphate donor. Isoenzyme 2: 35% of the activity with GTP | Euglena gracilis | ADP + NADP+ | - |
? | |
ATP + NAD+ | isoenzyme 1: most effective phosphate donor. Isoenzyme 2: 35% of the activity with GTP | Euglena gracilis Z | ADP + NADP+ | - |
? | |
CTP + NAD+ | isoenzyme 1: 55% of the activity with ATP. Isoenzyme 2: 77% of the activity with ATP | Euglena gracilis | CDP + NADP+ | - |
? | |
CTP + NAD+ | isoenzyme 1: 55% of the activity with ATP. Isoenzyme 2: 77% of the activity with ATP | Euglena gracilis Z | CDP + NADP+ | - |
? | |
GTP + NAD+ | - |
Euglena gracilis | GDP + NADP+ | - |
? | |
GTP + NAD+ | isoenzyme 1: 96% of the activity with ATP. Isoenzyme 2: most effective phosphoryl donor | Euglena gracilis | GDP + NADP+ | - |
? | |
GTP + NAD+ | - |
Euglena gracilis Z | GDP + NADP+ | - |
? | |
GTP + NAD+ | isoenzyme 1: 96% of the activity with ATP. Isoenzyme 2: most effective phosphoryl donor | Euglena gracilis Z | GDP + NADP+ | - |
? | |
UTP + NAD+ | isoenzyme 1: 12% of the activity with ATP. Isoenzyme 2: 19% of the activity with ATP | Euglena gracilis | UDP + NADP+ | - |
? | |
UTP + NAD+ | isoenzyme 1: 12% of the activity with ATP. Isoenzyme 2: 19% of the activity with ATP | Euglena gracilis Z | UDP + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Euglena gracilis |