Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Arabidopsis thaliana | |
Calmodulin | - |
Arabidopsis thaliana | |
Calmodulin | calmodulin-dependent isoform, full activation in presence of Ca2+ | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
analysis | studies of enzyme activation by calmodulin, calmodulin-like proteins, calcium-binding proteins, and calmodulin mutants | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
General Stability | Organism |
---|---|
unstable | Arabidopsis thaliana |
unstable, loses activity at room temperature overnight | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 12% inhibition at 0.2 mM, 50% inhibition at 2 mM | Arabidopsis thaliana | |
Trifluoperazine | calmodulin-dependent isoform, 50% inhibition at 0.057 mM, isoforms NADK1, NADK2, no effect | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
ATP | pH 7.9, 25°C | Arabidopsis thaliana | |
0.17 | - |
ATP | calmodulin-dependent isoform, pH 7.9, 25°C | Arabidopsis thaliana | |
0.17 | - |
ATP | ATP in form of MgATP2- | Arabidopsis thaliana | |
0.2 | - |
NAD+ | pH 7.9, 25°C | Arabidopsis thaliana | |
0.2 | - |
NAD+ | calmodulin-dependent isoform, pH 7.9, 25°C | Arabidopsis thaliana | |
0.43 | - |
NAD+ | pH 7.9, 25°C | Arabidopsis thaliana | |
0.43 | - |
NAD+ | isoform NADK2, pH 7.9, 25°C | Arabidopsis thaliana | |
0.52 | - |
NAD+ | pH 7.9, 25°C | Arabidopsis thaliana | |
0.52 | - |
NAD+ | isoform NADK1, pH 7.9, 25°C | Arabidopsis thaliana | |
0.73 | - |
ATP | pH 7.9, 25°C | Arabidopsis thaliana | |
0.73 | - |
ATP | isoform NADK1, pH 7.9, 25°C | Arabidopsis thaliana | |
0.73 | - |
ATP | ATP in form of MgATP2- | Arabidopsis thaliana | |
0.74 | - |
ATP | pH 7.9, 25°C | Arabidopsis thaliana | |
0.74 | - |
ATP | isoform NADK2, pH 7.9, 25°C | Arabidopsis thaliana | |
0.74 | - |
ATP | ATP in form of MgATP2- | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | putative | Arabidopsis thaliana | 9507 | - |
cytosol | putative | Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana | |
Mg2+ | required to stabilize phosphoanhydride bonds to ATP | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58200 | - |
- |
Arabidopsis thaliana |
109200 | - |
- |
Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | - |
known to be present in other plants | - |
Arabidopsis thaliana | - |
isoforms NADK1, NADK2 plus a calmodulin-dependent isoform isolated from seedling | - |
Purification (Comment) | Organism |
---|---|
partial | Arabidopsis thaliana |
recombinant isoforms NADK1, NADK2, partial purification of calmodulin-dependent isoform | Arabidopsis thaliana |
single-step calmodulin-affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | both isoforms NADK1 and NADK2 are present in all tissues examined and throughout development | Arabidopsis thaliana | - |
seedling | calmodulin-dependent isoform | Arabidopsis thaliana | - |
whole plant | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.1 | - |
pH 7.9, 25°C | Arabidopsis thaliana |
11.1 | - |
isoform NADK1, pH 7.9, 25°C | Arabidopsis thaliana |
14.3 | - |
pH 7.9, 25°C | Arabidopsis thaliana |
14.3 | - |
isoform NADK2, pH 7.9, 25°C | Arabidopsis thaliana |
213 | - |
- |
Arabidopsis thaliana |
213 | - |
calmodulin-depndent isoform, pH 7.9, 25°C | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
unstable | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NAD+ | - |
Arabidopsis thaliana | ADP + NADP+ | - |
? | |
ATP + NAD+ | UTP also may be used as phosphoryl donor | Arabidopsis thaliana | ADP + NADP+ | - |
? | |
additional information | NADP is not phosphorylated, tetrapolyphosphate or diphosphate are not used as phosphoryl donors | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Ca2+-dependent calmodulin-binding domain near N-terminal extension of isoform NADK2 | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
diphosphopyridine nucleotide kinase | - |
Arabidopsis thaliana |
diphosphppyridine kinase | - |
Arabidopsis thaliana |
NADK1 | calcium-calmodulin nonbinding isoform of plant NAD+ kinase | Arabidopsis thaliana |
NADK2 | calcium- and calmodulin-binding isoform of plant NAD+ kinase | Arabidopsis thaliana |
native plant calcium- and calmodulin-dependent NAD+-kinase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Arabidopsis thaliana |
7.9 | - |
isoforms NADK1, NADK2 | Arabidopsis thaliana |
8 | - |
- |
Arabidopsis thaliana |
8 | - |
calmodulin-dependent isoform | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
broad | Arabidopsis thaliana |
7.9 | - |
- |
Arabidopsis thaliana |