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Literature summary for 2.7.1.23 extracted from

  • Turner, W.L.; Waller, J.C.; Vanderbeld, B.; Snedden, W.A.
    Cloning and characterization of two NAD kinases from Arabidopsis. identification of a calmodulin binding isoform (2004), Plant Physiol., 135, 1243-1255.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Ca2+
-
Arabidopsis thaliana
Calmodulin
-
Arabidopsis thaliana
Calmodulin calmodulin-dependent isoform, full activation in presence of Ca2+ Arabidopsis thaliana

Application

Application Comment Organism
analysis studies of enzyme activation by calmodulin, calmodulin-like proteins, calcium-binding proteins, and calmodulin mutants Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

General Stability

General Stability Organism
unstable Arabidopsis thaliana
unstable, loses activity at room temperature overnight Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 12% inhibition at 0.2 mM, 50% inhibition at 2 mM Arabidopsis thaliana
Trifluoperazine calmodulin-dependent isoform, 50% inhibition at 0.057 mM, isoforms NADK1, NADK2, no effect Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
ATP pH 7.9, 25°C Arabidopsis thaliana
0.17
-
ATP calmodulin-dependent isoform, pH 7.9, 25°C Arabidopsis thaliana
0.17
-
ATP ATP in form of MgATP2- Arabidopsis thaliana
0.2
-
NAD+ pH 7.9, 25°C Arabidopsis thaliana
0.2
-
NAD+ calmodulin-dependent isoform, pH 7.9, 25°C Arabidopsis thaliana
0.43
-
NAD+ pH 7.9, 25°C Arabidopsis thaliana
0.43
-
NAD+ isoform NADK2, pH 7.9, 25°C Arabidopsis thaliana
0.52
-
NAD+ pH 7.9, 25°C Arabidopsis thaliana
0.52
-
NAD+ isoform NADK1, pH 7.9, 25°C Arabidopsis thaliana
0.73
-
ATP pH 7.9, 25°C Arabidopsis thaliana
0.73
-
ATP isoform NADK1, pH 7.9, 25°C Arabidopsis thaliana
0.73
-
ATP ATP in form of MgATP2- Arabidopsis thaliana
0.74
-
ATP pH 7.9, 25°C Arabidopsis thaliana
0.74
-
ATP isoform NADK2, pH 7.9, 25°C Arabidopsis thaliana
0.74
-
ATP ATP in form of MgATP2- Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast putative Arabidopsis thaliana 9507
-
cytosol putative Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Arabidopsis thaliana
Mg2+ required to stabilize phosphoanhydride bonds to ATP Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58200
-
-
Arabidopsis thaliana
109200
-
-
Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
-
known to be present in other plants
-
Arabidopsis thaliana
-
isoforms NADK1, NADK2 plus a calmodulin-dependent isoform isolated from seedling
-

Purification (Commentary)

Purification (Comment) Organism
partial Arabidopsis thaliana
recombinant isoforms NADK1, NADK2, partial purification of calmodulin-dependent isoform Arabidopsis thaliana
single-step calmodulin-affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information both isoforms NADK1 and NADK2 are present in all tissues examined and throughout development Arabidopsis thaliana
-
seedling calmodulin-dependent isoform Arabidopsis thaliana
-
whole plant
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.1
-
pH 7.9, 25°C Arabidopsis thaliana
11.1
-
isoform NADK1, pH 7.9, 25°C Arabidopsis thaliana
14.3
-
pH 7.9, 25°C Arabidopsis thaliana
14.3
-
isoform NADK2, pH 7.9, 25°C Arabidopsis thaliana
213
-
-
Arabidopsis thaliana
213
-
calmodulin-depndent isoform, pH 7.9, 25°C Arabidopsis thaliana

Storage Stability

Storage Stability Organism
unstable Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
Arabidopsis thaliana ADP + NADP+
-
?
ATP + NAD+ UTP also may be used as phosphoryl donor Arabidopsis thaliana ADP + NADP+
-
?
additional information NADP is not phosphorylated, tetrapolyphosphate or diphosphate are not used as phosphoryl donors Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
More Ca2+-dependent calmodulin-binding domain near N-terminal extension of isoform NADK2 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
diphosphopyridine nucleotide kinase
-
Arabidopsis thaliana
diphosphppyridine kinase
-
Arabidopsis thaliana
NADK1 calcium-calmodulin nonbinding isoform of plant NAD+ kinase Arabidopsis thaliana
NADK2 calcium- and calmodulin-binding isoform of plant NAD+ kinase Arabidopsis thaliana
native plant calcium- and calmodulin-dependent NAD+-kinase
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
Arabidopsis thaliana
7.9
-
isoforms NADK1, NADK2 Arabidopsis thaliana
8
-
-
Arabidopsis thaliana
8
-
calmodulin-dependent isoform Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
broad Arabidopsis thaliana
7.9
-
-
Arabidopsis thaliana