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Literature summary for 2.7.1.23 extracted from

  • Blomquist, C.H.
    Partial purification and characterization of nicotinamide adenine dinucleotide kinase from sea urchin eggs (1973), J. Biol. Chem., 248, 7044-7048.
    View publication on PubMed
Search for more literature for 2.7.1.23

Inhibitors

Inhibitors Comment Organism Structure
3',5'-cAMP 3.5 mM, 20% inhibition Strongylocentrotus purpuratus
adenosine diphosphoribose 3.5 mM, 31% inhibition Strongylocentrotus purpuratus
ADP 3.5 mM, 63% inhibition Strongylocentrotus purpuratus
AMP 3.5 mM, 29% inhibition Strongylocentrotus purpuratus
citrate 3.5 mM, 55% inhibition Strongylocentrotus purpuratus
Mn2+ above 1 mM Strongylocentrotus purpuratus
NADH
-
 Strongylocentrotus purpuratus
NADPH
-
 Strongylocentrotus purpuratus
phosphoenolpyruvate 3.5 mM, 20% inhibition Strongylocentrotus purpuratus
pyruvate 3.5 mM, 10% inhibition Strongylocentrotus purpuratus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 33% of the activation obtained with Mn2+ Strongylocentrotus purpuratus
Mg2+ divalent cation required Strongylocentrotus purpuratus
Mg2+ maximal activation at a Mg2+:ATP ratio of 1:1 Strongylocentrotus purpuratus
Mn2+ divalent cation required Strongylocentrotus purpuratus
Mn2+ at pH 6.5, with 2.0 mM NAD+ and 1.0 mM ATP maximal activity is observed with Mn2+ at 0.5 to 1.0 mM Strongylocentrotus purpuratus

Organism

Organism UniProt Comment Textmining
Strongylocentrotus purpuratus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Strongylocentrotus purpuratus

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Strongylocentrotus purpuratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.222
-
-
 Strongylocentrotus purpuratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NAD+
-
 Strongylocentrotus purpuratus ADP + NADP+
-
 ?
CTP + NAD+ 11% of the activity with ATP Strongylocentrotus purpuratus CDP + NADP+
-
 ?
GTP + NAD+ 14% of the activity with ATP Strongylocentrotus purpuratus GDP + NADP+
-
 ?
ITP + NAD+ 7% of the activity with ATP Strongylocentrotus purpuratus IDP + NADP+
-
 ?
UTP + NAD+ 5% of the activity with ATP Strongylocentrotus purpuratus UDP + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Strongylocentrotus purpuratus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 about 50% of maximal activity at pH 5.5 and pH 7.5 Strongylocentrotus purpuratus
6 7 activity decreases sharply below pH 6.0 and above pH 7.0 Strongylocentrotus purpuratus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 4°C, 18 h, about 60% loss of activity, irreversible loss of activity Strongylocentrotus purpuratus
6 7 4°C, 18 h, stable Strongylocentrotus purpuratus
8
-
 4°C, 18 h, about 50% loss of activity, loss of activity is reversible by dialysis to pH 6.2 Strongylocentrotus purpuratus
8.5
-
 4°C, 18 h, about 70% loss of activity, loss of activity is reversible by dialysis to pH 6.2 Strongylocentrotus purpuratus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Strongylocentrotus purpuratus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.013
-
 NADPH pH 6.5, 30°C Strongylocentrotus purpuratus
0.03
-
 NADH pH 6.5, 30°C Strongylocentrotus purpuratus