Information on EC 1.3.5.2 - dihydroorotate dehydrogenase (quinone)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.3.5.2
-
RECOMMENDED NAME
GeneOntology No.
dihydroorotate dehydrogenase (quinone)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-dihydroorotate + a quinone = orotate + a quinol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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pyrimidine metabolism
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Pyrimidine metabolism
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UMP biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(S)-dihydroorotate:quinone oxidoreductase
This Class 2 dihydroorotate dehydrogenase enzyme contains FMN [4]. The enzyme is found in eukaryotes in the mitochondrial membrane, and in some Gram negative bacteria associated with the cytoplasmic membrane [2,5]. The reaction is the only redox reaction in the de-novo biosynthesis of pyrimidine nucleotides [2,4]. The best quinone electron acceptors for the enzyme from bovine liver are ubiquinone-6 and ubiquinone-7, although simple quinones, such as benzoquinone, can also act as acceptor at lower rates [2]. Methyl-, ethyl-, tert-butyl and benzyl (S)-dihydroorotates are also substrates, but methyl esters of (S)-1-methyl and (S)-3-methyl and (S)-1,3-dimethyldihydroorotates are not [2]. Class 1 dihydroorotate dehydrogenases use either fumarate (EC 1.3.98.1), NAD+ (EC 1.3.1.14) or NADP+ (EC 1.3.1.15) as electron acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
59088-23-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
expression in Escherichia coli
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-
Manually annotated by BRENDA team
; family 1 enzyme A
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-
Manually annotated by BRENDA team
only enzyme A
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
tomato, cultured cells
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
DHODH is the fourth enzyme in the biosynthesis of pyrimidines
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-dihydroorotate + 1,4-naphthoquinone
orotate + 1,4-naphthoquinol
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
orotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone
orotate + 2,5-dimethyl-p-benzoquinol
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
show the reaction diagram
(S)-dihydroorotate + decylubiquinone
orotate + decylubiquinol
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + oxidized 2,6-dichloroindophenol
orotate + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
show the reaction diagram
(S)-dihydroorotate + ubiquinone-30
orotate + ubiquinol-30
show the reaction diagram
-
-
-
-
?
(S)-dihydroorotate + ubiquinone-50
orotate + ubiquinol-50
show the reaction diagram
-
-
-
-
?
benzyl-(S)-dihydroorotate + acceptor
benzyl orotate + reduced acceptor
show the reaction diagram
-
-
-
-
?
dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
orotate + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
show the reaction diagram
-
-
-
?
dihydroorotate + 2,5-dimethyl-p-benzoquinone
orotate + reduced 2,5-dimethyl-p-benzoquinone
show the reaction diagram
95% the rate of decylubiquinone, wild-type, 75% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
dihydroorotate + 2,5-dimethylbenzoquinone
orotate + reduced 2,5-dimethylbenzoquinone
show the reaction diagram
-
84% of the rate with decylubiquinone
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-
?
dihydroorotate + 2,6-dichloroindophenol
orotate + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
?
dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
dihydroorotate + acceptor
orotate + reduced acceptor
show the reaction diagram
dihydroorotate + coenzyme Q
orotate + reduced coenzyme Q
show the reaction diagram
-
coenzyme OD possesses a decyl tail
-
-
?
dihydroorotate + coenzyme Q1
orotate + reduced coenzyme Q1
show the reaction diagram
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coenzyme Q1 possesses a prenyl tail
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-
?
dihydroorotate + coenzyme QD
orotate + reduced coenzyme QD
show the reaction diagram
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coenzyme QD possesses a decyl tail
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-
?
dihydroorotate + CoQ0
orotate + reduced CoQo
show the reaction diagram
102% the rate of decylubiquinone, wild-type, 67% the rate of decylubiquinone, DELTA1-75 mutant
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-
?
dihydroorotate + decylplastoquinone
orotate + reduced decylplastoquinone
show the reaction diagram
dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
show the reaction diagram
dihydroorotate + menadione
orotate + reduced menadione
show the reaction diagram
dihydroorotate + O2
orotate + H2O2
show the reaction diagram
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-
-
-
?
dihydroorotate + potassium hexacyanoferrate(III)
orotate + reduced potassium hexacyanoferrate(III)
show the reaction diagram
-
-
-
?
dihydroorotate + ubiquinone 30
orotate + reduced ubiquinone 30
show the reaction diagram
-
-
-
?
dihydroorotate + ubiquinone 50
orotate + reduced ubiquinone 50
show the reaction diagram
-
-
-
?
dihydroorotate + ubiquinone-0
orotate + reduced ubiquinone-0
show the reaction diagram
-
80% of the rate with decylubiquinone
-
-
?
dihydroorotate + ubiquinone-10
orotate + reduced ubiquinone-10
show the reaction diagram
-
59% of the rate with decylubiquinone
-
-
?
dihydroorotate + ubiquinone-50
orotate + reduced ubiquinone-50
show the reaction diagram
83% the rate of decylubiquinone, wild-type, 76% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
ethyl-dihydroorotate + acceptor
ethyl-orotate + reduced acceptor
show the reaction diagram
-
-
-
-
?
L-dihydroorotate + 2,6-dichloroindophenol
orotate + ?
show the reaction diagram
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-
-
-
?
L-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
L-dihydroorotate + decylubiquinone
orotate + ?
show the reaction diagram
-
-
-
-
?
L-dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
show the reaction diagram
L-dihydroorotate + FMN
orotate + FMNH2
show the reaction diagram
L-dihydroorotate + ubiquinone-0
orotate + reduced ubiquinone-0
show the reaction diagram
-
-
-
?
L-dihydroorotate + ubiquinone-4
orotate + reduced ubiquinone-4
show the reaction diagram
-
-
-
?
L-dihydroorotate + ubiquinone-6
orotate + reduced ubiquinone-6
show the reaction diagram
L-dihydroorotate + vitamin K3
orotate + reduced vitamin K3
show the reaction diagram
-
-
-
?
methyl-dihydroorotate + acceptor
methyl-orotate + reduced acceptor
show the reaction diagram
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-
-
-
?
tert-butyl-dihydroorotate + acceptor
tert-butyl-orotate + reduced acceptor
show the reaction diagram
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-
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
show the reaction diagram
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
show the reaction diagram
dihydroorotate + acceptor
orotate + reduced acceptor
show the reaction diagram
L-dihydroorotate + FMN
orotate + FMNH2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-methyl-1,4-naphthoquinone
little catalytic efficiency, N-terminally truncated protein
flavin
ubiquinone
-
the mitochondrial enzyme requires oxidized ubiquinone, CoQ
ubiquinone-0
little catalytic efficiency, N-terminally truncated protein
Ubiquinone-10
CoQD, efficient, N-terminally truncated protein
ubiquinone-4
efficient, N-terminally truncated protein
ubiquinone-8
efficient, N-terminally truncated protein
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
-
iron-zinc protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2,3-difluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(2,5-difluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
(2,6-difluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(2-chloro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(2-fluoro-4-methyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(2E)-2-cyano-3-hydroxy-N-[4-(trifluoromethyl)phenyl]but-2-enamide
-
50% inhibition at 0.000435 mM
(2Z)-2-cyano-3-cyclopropyl-3-hydroxy-N-[4-(trifluoromethyl)phenyl]prop-2-enamide
(2Z)-2-cyano-N-(2',3-dichlorobiphenyl-4-yl)-3-hydroxybut-2-enamide
(2Z)-2-cyano-N-(2,2'-dichlorobiphenyl-4-yl)-3-hydroxybut-2-enamide
(2Z)-2-cyano-N-(2,3'-dichlorobiphenyl-4-yl)-3-hydroxybut-2-enamide
(2Z)-2-cyano-N-(3'-ethoxybiphenyl-4-yl)-3-hydroxybut-2-enamide
(2Z)-N-(2'-chlorobiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
(2Z)-N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
(2Z)-N-(biphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
(2Z)-N-[2'-chloro-3-(trifluoromethyl)biphenyl-4-yl]-2-cyano-3-hydroxybut-2-enamide
(3,4-difluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(3,4-dimethyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(3,5-bis-trifluoromethyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(3,5-difluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(3-chloro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(3-fluoro-4-methyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(3-fluoro-4-trifluoromethyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(3-fluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(4-benzyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
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(4-bromo-3-trifluoromethyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(4-bromo-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
(4-chloro-3-trifluoromethyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
(4-chloro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
(4-difluoromethoxy-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(4-fluoro-3-methyl-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
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-
-
(4-fluoro-phenyl)-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
(4-methoxy-phenyl)(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(2,3,4,5,6-pentafluorophenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(2,3,4-trifluoro-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(2,3,5,6-tetrafluoro-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(2,4,5-trifluoro-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(2,4,6-trifluoro-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(2-trifluoromethyl-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(3-trifluoromethyl-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(4-methyl-3-trifluoromethylphenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(4-nitro-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(4-trifluoromethoxy-phenyl)-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-(4-trifluoromethyl-phenyl)-amine
-
DSM74
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-m-tolyl-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-naphthalen-2-yl-amine
-
DSM1, a potent triazolopyrimidine-based inhibitor of PfDHODH that shows greater than 4000fold selectivity for the malarial enzyme when compared to human DHODH
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-o-tolyl-amine
-
-
-
(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-p-tolyl-amine
-
-
-
(R)-dihydroorotate
-
-
1,10-phenanthroline
-
-
1-(1-naphthyl)-3-[2-(trifluoromethyl)phenyl]urea
-
50% inhibition at 0.0004 mM, wild type, 0.00034 mM, mutant R265A, above 0.2, mutant H185A
1-(2-methoxyphenyl)-3-(1-naphthyl)urea
-
50% inhibition at 0.00023 mM, wild type, 0.00024 mM, mutant R265A, 0.140, mutant H185A
1-(2-methoxyphenyl)-3-naphthalen-1-ylurea
1-(4-chlorophenyl)-3-quinolin-8-ylurea
-
50% inhibition at 0.00078 mM, wild type, 0.002 mM, mutant R265A, above 0.4, mutant H185A
1-hydroxy-2-dodecyl-4(1)quinolone
-
competitive with regard to decylubiquinone, uncompetitive with regard to dihydroorotate
2-(4'-tert-butyl-2-chloro-biphenyl-4-ylcarbamoyl)-cyclopent-1-enecarboxylic acid
-
50% inhibition at 0.000080 mM
2-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-ylamino)-benzonitrile
-
-
-
2-([[2,3,5,6-tetrafluoro-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000007 mM
2-([[2-chloro-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000020 mM
2-([[2-chloro-4'-(dimethylamino)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00005 mM
2-([[3'-(trifluoromethoxy)-3-(trifluoromethyl)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000840 mM
2-([[3,5-difluoro-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000033 mM
2-([[3-chloro-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000290 mM
2-([[3-cyano-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000370 mM
2-([[3-fluoro-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00028 mM
2-([[3-fluoro-4'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00570 mM
2-([[3-methyl-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000150 mM
2-([[3-nitro-3'-(trifluoromethoxy)biphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000390 mM
2-([[4'-(dimethylamino)-3,5-difluorobiphenyl-4-yl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00061 mM
2-([[4-(1-naphthyl)-2-(trifluoromethyl)phenyl]amino]carbonyl)cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000180 mM
2-cyano-3-(9-ethyl-9H-carbazol-3-ylamino)acrylic acid ethyl ester
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(cyano)phenyl]-propenamide
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(nitro)phenyl]-propenamide
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(trifluoromethyl)phenyl]-propenamide
2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide
-
-
2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide
-
A177-1726, active derivative of human inhibitor leflunomide
2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide
2-methyl-3-nitro-N-[3-(trifluoromethyl)phenyl]benzamide
-
50% inhibition at 0.00008 mM, wild type, 0.00037 mM, mutant R265A, 0.120, mutant H185A
2-methyl-N-1-naphthyl-3-nitrobenzamide
-
50% inhibition at 0.00008 mM, wild type, 0.00048 mM, mutant R265A, 0.180, mutant H185A
2-[(2,5-dichlorophenyl)sulfanyl]-5-ethyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
2-[(2,5-dichlorophenyl)sulfanyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
2-[(3,4-dichlorophenyl)sulfanyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
2-[(4-chlorophenyl)sulfanyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
2-[(biphenyl-4-ylamino)carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00041 mM
2-[(E)-2-(2-methylphenyl)ethenyl]quinolin-4-ol
2-[[(2',3,5-trifluorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000045 mM
2-[[(2'-chloro-3,5-difluorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000018 mM
2-[[(2'-chlorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000225 mM
2-[[(2,3,5,6-tetrafluoro-2'-methoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000008 mM
2-[[(3'-ethoxy-3,5-difluorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000017 mM
2-[[(3,3'-dimethoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000170 mM
2-[[(3,5-difluoro-2',4'-dimethoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000090 mM
2-[[(3,5-difluoro-2'-methoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000042 mM
2-[[(3-chloro-2'-methoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000310 mM
2-[[(3-chloro-4'-methoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00840 mM
2-[[(3-fluoro-3'-hydroxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000610 mM
-
2-[[(3-fluoro-4'-methoxybiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.00170 mM
2-[[(4'-bromo-2-chlorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000070 mM
2-[[(4'-bromo-3-chlorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.002900 mM
2-[[(4'-bromo-3-fluorobiphenyl-4-yl)amino]carbonyl]cyclopent-1-ene-1-carboxylic acid
-
50% inhibition at 0.000735 mM
3-hydroxy-2-(3,3-dichloroallyl)-1,4-naphthoquinone
4-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-ylamino)-2-trifluoromethylbenzonitrile
-
-
-
4-pentyl-1,3,4,10-tetrahydroacridin-9(2H)-one
4-[(5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)amino]-2-(trifluoromethyl)benzonitrile
-
-
5,6-dimethyl-N-(naphthalen-2-yl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
5-(1H-benzimidazol-1-yl)-N-propylthiophene-2-carboxamide
5-(1H-indol-1-yl)-N-propylthiophene-2-carboxamide
5-(2-methyl-1H-benzimidazol-1-yl)-N-propylthiophene-2-carboxamide
5-(2-methyl-1H-indol-1-yl)-N-propylthiophene-2-carboxamide
5-(4-methylpiperazin-1-yl)-N-propylthiophene-2-carboxamide
5-(piperidin-1-yl)-N-propylthiophene-2-carboxamide
5-aminoorotate
-
-
5-bromoorotate
-
-
5-ethyl-N-(naphthalen-2-yl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
5-Fluoroorotate
-
-
5-iodoorotate
-
-
5-methoxy-2-[(4-phenoxyphenyl)amino]benzoic acid
-
-
5-methyl-7-(naphthalen-2-yloxy)[1,2,4]triazolo[1,5-a]pyrimidine
5-methyl-7-(naphthalen-2-ylsulfanyl)[1,2,4]triazolo[1,5-a]pyrimidine
5-methyl-N-(2,3,4-trifluorophenyl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-(2,4,5-trifluorophenyl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-(3-methylphenyl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-(4-methylphenyl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-(4-nitrophenyl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-(naphthalen-2-yl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
5-methyl-N-[3-(trifluoromethyl)phenyl][1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-[4-(trifluoromethoxy)phenyl][1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-methyl-N-[4-(trifluoromethyl)phenyl][1,2,4]triazolo[1,5-a]pyrimidin-7-amine
5-methyl-N-[4-methyl-3-(trifluoromethyl)phenyl][1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
5-Methylorotate
6-chloro-2-(2'-fluorobiphenyl-4-yl)quinoline-4-carboxylic acid
-
-
-
6-methyl-N-phenyl-3aH-inden-4-amine
A77 1726
A77-1726
-
50% inhibition at 0.00045 mM, wild-type, 0.00033 mM, DELTA2-21 mutant, 0.00031 mM, DELTA22-37 mutant, 0.00036 mM, DELTA2-37 mutant
atovaquone
Barbituric acid
benzoquinones
-
ubiquinone-0 and decylubiquinone
-
biphenyl-4-yl-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)-amine
-
-
-
brequinar
brequinar sodium
chloronaphthoquinone
-
-
cis-5-methyldihydroorotate
-
-
deoxycholate
-
-
dichloroallyl lawsone
-
50% inhibition at 0.00051 mM, wild-type, 0.00031 mM, DELTA2-21 mutant, 0.00020 mM, DELTA22-37 mutant, 0.00021 mM, DELTA2-37 mutant
dichloroallyl-lawsone
diethyl [(1H-benzimidazol-5-ylamino)methylidene]propanedioate
-
-
diethyl [(1H-indazol-5-ylamino)methylidene]propanedioate
-
-
diethyl [(5a,9a-dihydrodibenzo[b,d]thiophen-2-ylamino)methylidene]propanedioate
-
-
diethyl [(biphenyl-3-ylamino)methylidene]propanedioate
-
-
diethyl [(biphenyl-4-ylamino)methylidene]propanedioate
-
-
diethyl [(dibenzo[b,d]thiophen-2-ylamino)methylidene]propanedioate
diethyl {[(2-methylphenyl)amino]methylidene}propanedioate
-
-
diethyl {[(4-methylphenyl)amino]methylidene}propanedioate
-
-
diethyldicarbonate
Dodecyl sulfate
-
-
ethyl (2Z)-3-(biphenyl-3-ylamino)-2-cyanoprop-2-enoate
-
-
ethyl (2Z)-3-(biphenyl-4-ylamino)-2-cyanoprop-2-enoate
-
-
ethyl 4-(4-ethoxybenzyl)-3,5-dimethyl-1H-pyrrole-2-carboxylate
fatty acids
Genz-667348
Genz-668857
Genz-669178
H2O2
-
byproduct, tends to inactivate enzyme
HR325
-
structural analogue of A77 1726
-
Lapachol
Lawsone
MNA 279
MNA 715
N-(2,3-difluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(2,4-dichlorophenyl)-2-naphthamide
-
50% inhibition at 0.00005 mM, wild type, 0.00008 mM, mutant R265A, above 0.05, mutant H185A
N-(2,4-dichlorophenyl)naphthalene-2-carboxamide
N-(2-chlorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(2-fluoro-4-methylphenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(2-fluorophenyl)-2-naphthamide
-
50% inhibition at 0.00047 mM, wild type, 0.00030 mM, mutant R265A, 0.21, mutant H185A
N-(2-fluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(3,4-dichlorophenyl)-2-methyl-3-nitrobenzamide
-
50% inhibition at 0.00008 mM, wild type, 0.0028 mM, mutant R265A, 0.06, mutant H185A
N-(3,4-difluorophenyl)-2-methyl-3-nitrobenzamide
-
50% inhibition at 0.00026 mM, wild type, 0.00082 mM, mutant R265A, 0.25, mutant H185A
N-(3,4-difluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(3,4-dimethylphenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide
-
-
N-(3,5-dichlorophenyl)-2-methyl-3-nitrobenzamide
N-(3,5-difluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(3-bromophenyl)-2-methyl-3-nitrobenzamide
-
50% inhibition at 0.00006 mM, wild type, 0.0012 mM, mutant R265A, 0.140, mutant H185A
N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
-
-
N-(3-chloro-4-fluorophenyl)-2-methyl-3-nitrobenzamide
-
50% inhibition at 0.0001 mM, wild type, 0.0005 mM, mutant R265A, 0.13, mutant H185A
N-(3-chlorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(3-fluoro-4-methylphenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(3-fluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(4-benzylphenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(4-bromo-2-methylphenyl)-2-naphthamide
-
50% inhibition at 0.00005 mM, wild type, 0.00005 mM, mutant R265A, 0.57, mutant H185A
N-(4-bromophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(4-chlorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(4-fluoro-3-methylphenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(4-fluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(4-methoxyphenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-(anthracen-2-yl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(anthracen-2-yl)-N,5-dimethyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-(biphenyl-4-yl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-anthracen-2-yl-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
i.e. DSM2
N-cyclopropyl-5-(2,3-dihydro-4H-1,4-benzothiazin-4-yl)thiophene-2-carboxamide
N-propyl-5-(1H-pyrazol-1-yl)thiophene-2-carboxamide
N-[3-fluoro-4-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
N-[4-(difluoromethoxy)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-[4-bromo-3-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
N-[4-chloro-3-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
-
-
Orotate
redoxal
rhodamine 123
-
mitochondrial inhibitor
teriflunomide
-
a redox-silent coenzyme Q antagonist of DHODH
Thenoyltrifluoroacetone
toltrazuril
[2-fluoro-2',5'-dimethyl-4'-[6-(3-methyl-2-butenyloxy)pyridin-3-yl]biphenyl-4-yl]-(3-methyl-2-butenyl)amine
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
oxygen
seems to promote very low DHODH activity
additional information
-
activity of the bc1 complex is essential for providing oxidized ubiqinone to DHODH for the formation of pyrimidines
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 0.265
(S)-dihydroorotate
0.028
1,4-Naphthoquinone
-
pH 8.0, 30C
0.099
2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
pH 8.0, 30C
0.132
2,5-dimethyl-p-benzoquinone
-
pH 8.0, 30C
0.0146
2,6-dichlorophenolindophenol
-
S175C mutant
0.0058
2,6-dihydrophenolindophenol
-
-
7.8 - 48
coenzyme Q1
0.0011 - 0.022
coenzyme Q6
0.0013
coenzyme Q7
-
cosubstrate benzyl-S-dihydroorotate
8.8 - 53
coenzyme QD
0.0095
decyclubiquinone
-
recombinant enzyme
0.0065 - 0.341
decylubiquinone
0.004 - 0.122
dihydroorotate
0.015 - 0.123
L-dihydroorotate
0.184
menadione
-
-
0.0154 - 0.1542
methyl dihydroorotate
0.0019
methyl-S-dihydroorotate
-
-
-
0.0062 - 0.115
S-dihydroorotate
0.007 - 0.062
ubiquinone
0.04 - 0.075
ubiquinone-0
0.051
ubiquinone-30
-
pH 8.0, 30C
0.035
ubiquinone-4
pH 8.0, 25C, cosubstrate L-dihydroorotate, N-terminally truncated protein
0.01 - 0.027
ubiquinone-50
0.0225 - 0.062
ubiquinone-6
additional information
additional information
-
relative Km-values for wild-type and mutants listed for (S)-dihydroorotate and ubiquinone-6
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
38 - 86
(S)-dihydroorotate
29
1,4-Naphthoquinone
Toxoplasma gondii
-
pH 8.0, 30C
32
2,3-dimethoxy-5-methyl-1,4-benzoquinone
Toxoplasma gondii
-
pH 8.0, 30C
44
2,5-dimethyl-p-benzoquinone
Toxoplasma gondii
-
pH 8.0, 30C
5.7 - 9.3
Abz-VAA
0.19 - 7.8
coenzyme Q1
44.2
coenzyme Q6
Bos taurus
-
-
0.12 - 12
coenzyme QD
1.8 - 89
decylubiquinone
1.4 - 72.9
dihydroorotate
1.7 - 16.1
L-dihydroorotate
18.3
menadione
Bos taurus
-
-
45.4
methyl-dihydroorotate
Bos taurus
-
-
0.083 - 0.63
O2
0.25 - 95
S-dihydroorotate
21
ubiquinone-30
Toxoplasma gondii
-
pH 8.0, 30C
1.8
ubiquinone-4
Plasmodium falciparum
Q08210
pH 8.0, 25C, cosubstrate L-dihydroorotate, N-terminally truncated protein
61
ubiquinone-50
Toxoplasma gondii
-
pH 8.0, 30C
2
ubiquinone-6
Plasmodium falciparum
Q08210
pH 8.0, 25C, cosubstrate L-dihydroorotate, N-terminally truncated protein
0.36
ubiquinone6
Plasmodium falciparum
-
-
additional information
additional information
Homo sapiens
-
relative turnover numbers for wild-type and mutants listed for (S)-dihydroorotate and ubiquinone6
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
140 - 1100
(S)-dihydroorotate
2300 - 2500
decylubiquinone
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00005 - 0.0604
2-cyano-3-(9-ethyl-9H-carbazol-3-ylamino)acrylic acid ethyl ester
0.4
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(nitro)phenyl]-propenamide
pH 8.0, 25C, varied substrate QD, N-terminally truncated protein
0.0002 - 0.001
2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide
0.16 - 0.25
3-hydroxy-2-(3,3-dichloroallyl)-1,4-naphthoquinone
0.0244
5-aminoorotate
-
-
0.1408
5-bromoorotate
-
-
0.0151
5-Fluoroorotate
-
-
0.2246
5-iodoorotate
-
-
0.1185
5-Methylorotate
-
-
0.000698 - 0.015
atovaquone
0.092
Barbiturate
-
-
0.003
Barbituric acid
-
-
0.0000232 - 0.0000265
brequinar sodium
0.00001 - 0.000067
dichloro-allyllawsone
0.000061 - 0.000618
Lapachol
0.049 - 0.5
Lawsone
0.0134 - 0.0388
Orotate
0.005
Orotic acid
-
competitive to dihydroorotate
0.059 - 0.086
redoxal
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000117 - 0.0925
(2Z)-2-cyano-3-cyclopropyl-3-hydroxy-N-[4-(trifluoromethyl)phenyl]prop-2-enamide
0.00018 - 0.004
(2Z)-2-cyano-N-(2',3-dichlorobiphenyl-4-yl)-3-hydroxybut-2-enamide
0.000022 - 0.165
(2Z)-2-cyano-N-(2,2'-dichlorobiphenyl-4-yl)-3-hydroxybut-2-enamide
0.00019 - 0.2366
(2Z)-2-cyano-N-(2,3'-dichlorobiphenyl-4-yl)-3-hydroxybut-2-enamide
0.00013 - 0.182
(2Z)-2-cyano-N-(3'-ethoxybiphenyl-4-yl)-3-hydroxybut-2-enamide
0.0474
(2Z)-N-(2'-chlorobiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
Plasmodium falciparum
-
-
-
0.0002 - 0.0086
(2Z)-N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
0.00009 - 0.0257
(2Z)-N-(biphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
0.00017 - 0.0062
(2Z)-N-[2'-chloro-3-(trifluoromethyl)biphenyl-4-yl]-2-cyano-3-hydroxybut-2-enamide
0.00023 - 0.11
1-(2-methoxyphenyl)-3-naphthalen-1-ylurea
0.554 - 1.635
1-hydroxy-2-dodecyl-4(1)quinolone
0.00044 - 0.491
2-cyano-3-(9-ethyl-9H-carbazol-3-ylamino)acrylic acid ethyl ester
0.096
2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide
Toxoplasma gondii
-
pH 8.0, 30C
0.091
2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide
Toxoplasma gondii
-
pH 8.0, 30C
0.000013 - 0.1
2-[(2,5-dichlorophenyl)sulfanyl]-5-ethyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
0.000051 - 0.011
2-[(2,5-dichlorophenyl)sulfanyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
0.0019 - 0.0065
2-[(3,4-dichlorophenyl)sulfanyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
0.001 - 0.039
2-[(4-chlorophenyl)sulfanyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-ol
0.00093 - 0.03
2-[(E)-2-(2-methylphenyl)ethenyl]quinolin-4-ol
0.00011 - 0.03
4-pentyl-1,3,4,10-tetrahydroacridin-9(2H)-one
0.00016
5,6-dimethyl-N-(naphthalen-2-yl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.00047 - 0.0007
5-(1H-benzimidazol-1-yl)-N-propylthiophene-2-carboxamide
0.00046 - 0.00071
5-(1H-indol-1-yl)-N-propylthiophene-2-carboxamide
0.00017 - 0.00063
5-(2-methyl-1H-benzimidazol-1-yl)-N-propylthiophene-2-carboxamide
0.000042 - 0.03
5-(2-methyl-1H-indol-1-yl)-N-propylthiophene-2-carboxamide
0.01 - 0.03
5-(4-methylpiperazin-1-yl)-N-propylthiophene-2-carboxamide
0.03
5-(piperidin-1-yl)-N-propylthiophene-2-carboxamide
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.00019
5-ethyl-N-(naphthalen-2-yl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.000081
5-methoxy-2-[(4-phenoxyphenyl)amino]benzoic acid
Homo sapiens
-
pH 8.0, 22C
0.0017
5-methyl-7-(naphthalen-2-yloxy)[1,2,4]triazolo[1,5-a]pyrimidine
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.1
5-methyl-7-(naphthalen-2-ylsulfanyl)[1,2,4]triazolo[1,5-a]pyrimidine
Plasmodium falciparum
-
above, pH not specified in the publication, temperature not specified in the publication
0.000047 - 0.2
5-methyl-N-(naphthalen-2-yl)[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.00028 - 0.025
5-methyl-N-[4-(trifluoromethyl)phenyl][1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.000015
6-chloro-2-(2'-fluorobiphenyl-4-yl)quinoline-4-carboxylic acid
Homo sapiens
-
pH 8.0, 22C
-
0.1
6-methyl-N-phenyl-3aH-inden-4-amine
Plasmodium falciparum
-
above, pH not specified in the publication, temperature not specified in the publication
0.00016 - 0.03
diethyl [(dibenzo[b,d]thiophen-2-ylamino)methylidene]propanedioate
0.00004 - 0.03
ethyl 4-(4-ethoxybenzyl)-3,5-dimethyl-1H-pyrrole-2-carboxylate
0.000014 - 0.000042
Genz-667348
0.000012 - 0.000044
Genz-668857
0.000015 - 0.00005
Genz-669178
0.00005 - 0.05
N-(2,4-dichlorophenyl)naphthalene-2-carboxamide
0.1
N-(2-chlorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.049 - 12
N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide
0.000016 - 0.2
N-(3,5-dichlorophenyl)-2-methyl-3-nitrobenzamide
0.06
N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
Toxoplasma gondii
-
pH 8.0, 30C
0.00068 - 0.0014
N-(3-chlorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.0005 - 0.0092
N-(3-fluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.00078
N-(4-bromophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.019
N-(4-fluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.000056 - 0.0007
N-(anthracen-2-yl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.003
N-(anthracen-2-yl)-N,5-dimethyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
Plasmodium falciparum
-
pH not specified in the publication, temperature not specified in the publication
0.00021 - 0.0044
N-anthracen-2-yl-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
0.00025 - 0.00038
N-cyclopropyl-5-(2,3-dihydro-4H-1,4-benzothiazin-4-yl)thiophene-2-carboxamide
0.01 - 0.03
N-propyl-5-(1H-pyrazol-1-yl)thiophene-2-carboxamide
0.000077 - 0.00021
N-[3-fluoro-4-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine
additional information
A77 1726
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002672
-
37C
0.0026
0.0054
0.0066
-
heart
0.0078
-
heart
0.015
-
EAT cells
1.1 - 23
-
with 0.5 mM fumarate
1.1 - 7.7
-
with O2
1.1 - 23
-
with 0.5 mM fumarate
1.1 - 7.7
-
with O2
1.49
-
-
6
using decylubiquinone and 2,6-dichloroindophenol as acceptor
7.4 - 58
9.1 - 14.5
9.6
-
recombinant enzyme, electron acceptor decylubiquinone
20 - 24
83
-
expressed in Escherichia coli
84
-
pH 8.0, 30C
99
-
with L-dihydroorotate and 2,6-dichloroindophenol as substrates, at 30C
103
-
-
130 - 150
-
-
130
-
expressed in SF21 insect cells
150
-
; expressed in Escherichia coli
119000
-
cosubstrate O2, pH 7.5
245000
-
cosubstrate 2,6-dichlorophenolindophenol, pH 7.5
548000
-
cosubstrate Q1, pH 7.5
additional information
highest activities in ileum and colon
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
-
particulate enzyme
7.1
-
dihydroorotate + 2,6-dichlorophenolindophenol
8 - 8.1
8 - 8.1
-
-
8 - 8.1
8.5
-
recombinant enzyme
additional information