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Literature summary for 1.3.5.2 extracted from

  • Hines, V.; Keys, L.D.; Johnston, M.
    Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase [published erratum appears in J Biol Chem 1987 Nov 5;262(31):15322] (1986), J. Biol. Chem., 261, 11386-11392.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(R)-dihydroorotate
-
Bos taurus
5-Methylorotate
-
Bos taurus
cis-5-methyldihydroorotate
-
Bos taurus
Orotate competitive to dihydroorotate Bos taurus
Thenoyltrifluoroacetone
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
coenzyme Q6
-
Bos taurus
0.0013
-
coenzyme Q7 cosubstrate benzyl-S-dihydroorotate Bos taurus
0.0019
-
methyl-S-dihydroorotate
-
Bos taurus
0.0092
-
S-dihydroorotate
-
Bos taurus
0.184
-
menadione
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
SDS-PAGE, glycerol gradient ultracentrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
103
-
-
Bos taurus

Storage Stability

Storage Stability Organism
4°C, pH 9.0, 50 mM Tris/HCl buffer, 0.1% Triton X-100, 3-4 weeks, t1/2: 2.5 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + coenzyme Q7
-
Bos taurus orotate + reduced coenzyme Q7
-
r
(S)-dihydroorotate + menadione
-
Bos taurus orotate + menadiol
-
r
(S)-dihydroorotate + ubiquinone
-
Bos taurus orotate + ubiquinol
-
?
benzyl-(S)-dihydroorotate + acceptor
-
Bos taurus benzyl orotate + reduced acceptor
-
?
dihydroorotate + acceptor acceptor: quinones Bos taurus orotate + reduced acceptor
-
?
dihydroorotate + acceptor acceptor: coenzyme Q6 Bos taurus orotate + reduced acceptor
-
?
dihydroorotate + acceptor not acceptor: oxygen Bos taurus orotate + reduced acceptor
-
?
dihydroorotate + acceptor acceptor coenzyme Q7 Bos taurus orotate + reduced acceptor
-
?
ethyl-dihydroorotate + acceptor
-
Bos taurus ethyl-orotate + reduced acceptor
-
?
L-dihydroorotate + ubiquinone-6
-
Bos taurus orotate + reduced ubiquinone-6
-
?
methyl-dihydroorotate + acceptor
-
Bos taurus methyl-orotate + reduced acceptor
-
?
methyl-S-dihydroorotate + 2,6-dichlorophenolindophenol
-
Bos taurus methylorotate + reduced 2,6-dichlorophenolindophenol
-
r
additional information not substrate: 1-methyl-(S)-dihydroorotate, 3-methyl-dihydroorotate, 1,3-dimethyldihydroorotate Bos taurus ?
-
?
tert-butyl-dihydroorotate + acceptor
-
Bos taurus tert-butyl-orotate + reduced acceptor
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE, glycerol gradient centrifugation Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
t1/2: 2.5 months Bos taurus
37
-
3 h Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 no activity below pH 6.0 and above pH 10.0 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
unstable below Bos taurus
6.5 10.5 stable Bos taurus