Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-dihydroorotate | - |
Bos taurus | |
5-Methylorotate | - |
Bos taurus | |
cis-5-methyldihydroorotate | - |
Bos taurus | |
Orotate | competitive to dihydroorotate | Bos taurus | |
Thenoyltrifluoroacetone | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
coenzyme Q6 | - |
Bos taurus | |
0.0013 | - |
coenzyme Q7 | cosubstrate benzyl-S-dihydroorotate | Bos taurus | |
0.0019 | - |
methyl-S-dihydroorotate | - |
Bos taurus | |
0.0092 | - |
S-dihydroorotate | - |
Bos taurus | |
0.184 | - |
menadione | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE, glycerol gradient ultracentrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
103 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, pH 9.0, 50 mM Tris/HCl buffer, 0.1% Triton X-100, 3-4 weeks, t1/2: 2.5 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + coenzyme Q7 | - |
Bos taurus | orotate + reduced coenzyme Q7 | - |
r | |
(S)-dihydroorotate + menadione | - |
Bos taurus | orotate + menadiol | - |
r | |
(S)-dihydroorotate + ubiquinone | - |
Bos taurus | orotate + ubiquinol | - |
? | |
benzyl-(S)-dihydroorotate + acceptor | - |
Bos taurus | benzyl orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: quinones | Bos taurus | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: coenzyme Q6 | Bos taurus | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | not acceptor: oxygen | Bos taurus | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor coenzyme Q7 | Bos taurus | orotate + reduced acceptor | - |
? | |
ethyl-dihydroorotate + acceptor | - |
Bos taurus | ethyl-orotate + reduced acceptor | - |
? | |
L-dihydroorotate + ubiquinone-6 | - |
Bos taurus | orotate + reduced ubiquinone-6 | - |
? | |
methyl-dihydroorotate + acceptor | - |
Bos taurus | methyl-orotate + reduced acceptor | - |
? | |
methyl-S-dihydroorotate + 2,6-dichlorophenolindophenol | - |
Bos taurus | methylorotate + reduced 2,6-dichlorophenolindophenol | - |
r | |
additional information | not substrate: 1-methyl-(S)-dihydroorotate, 3-methyl-dihydroorotate, 1,3-dimethyldihydroorotate | Bos taurus | ? | - |
? | |
tert-butyl-dihydroorotate + acceptor | - |
Bos taurus | tert-butyl-orotate + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE, glycerol gradient centrifugation | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
t1/2: 2.5 months | Bos taurus |
37 | - |
3 h | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | no activity below pH 6.0 and above pH 10.0 | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
unstable below | Bos taurus |
6.5 | 10.5 | stable | Bos taurus |