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Literature summary for 1.3.5.2 extracted from

  • Loffler, M.; Knecht, W.; Rawls, J.; Ullrich, A.; Dietz, C.
    Drosophila melanogaster dihydroorotate dehydrogenase: the N-terminus is important for biological function in vivo but not for catalytic properties in vitro (2002), Insect Biochem. Mol. Biol., 32, 1159-1169.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
DELTA2-21 instable in vivo Drosophila melanogaster
DELTA2-37 instable in vivo Drosophila melanogaster
DELTA22-37 instable in vivo Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
A77-1726 50% inhibition at 0.00045 mM, wild-type, 0.00033 mM, DELTA2-21 mutant, 0.00031 mM, DELTA22-37 mutant, 0.00036 mM, DELTA2-37 mutant Drosophila melanogaster
brequinar 50% inhibition at 0.567 mM, wild-type, 0.528 mM, DELTA2-21 mutant, 0.615 mM, DELTA22-37 mutant, 0.662 mM, DELTA2-37 mutant Drosophila melanogaster
dichloroallyl lawsone 50% inhibition at 0.00051 mM, wild-type, 0.00031 mM, DELTA2-21 mutant, 0.00020 mM, DELTA22-37 mutant, 0.00021 mM, DELTA2-37 mutant Drosophila melanogaster
redoxal 50% inhibition at 0.029 mM, wild-type, 0.057 mM, DELTA2-21 mutant, 0.040 mM, DELTA22-37 mutant, 0.056 mM, DELTA2-37 mutant Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
decylubiquinone wild-type recombinant enzyme Drosophila melanogaster
0.0113
-
decylubiquinone DELTA22-37 mutant enzyme Drosophila melanogaster
0.0115
-
decylubiquinone DELTA2-21 mutant enzyme Drosophila melanogaster
0.0118
-
decylubiquinone DELTA2-37 mutant enzyme Drosophila melanogaster
0.0164
-
dihydroorotate DELTA2-21 mutant enzyme Drosophila melanogaster
0.0166
-
dihydroorotate DELTA22-37 mutant enzyme Drosophila melanogaster
0.0167
-
dihydroorotate DELTA2-37 mutant enzyme Drosophila melanogaster
0.017
-
dihydroorotate wild-type recombinant enzyme Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, His8-tag Drosophila melanogaster

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.6
-
recombinant enzyme, electron acceptor decylubiquinone Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + decylubiquinone
-
Drosophila melanogaster orotate + decylubiquinol
-
r
dihydroorotate + 2,5-dimethylbenzoquinone 84% of the rate with decylubiquinone Drosophila melanogaster orotate + reduced 2,5-dimethylbenzoquinone
-
?
dihydroorotate + 2,6-dichlorophenolindophenol 33% of the rate with decylubiquinone Drosophila melanogaster orotate + reduced 2,6-dichlorophenolindophenol
-
?
dihydroorotate + decylplastoquinone 78% of the rate with decylubiquinone Drosophila melanogaster orotate + reduced decylplastoquinone
-
?
dihydroorotate + decylubiquinone
-
Drosophila melanogaster orotate + reduced decylubiquinone
-
?
dihydroorotate + menadione 39% of the rate with decylubiquinone Drosophila melanogaster orotate + reduced menadione
-
?
dihydroorotate + ubiquinone-0 80% of the rate with decylubiquinone Drosophila melanogaster orotate + reduced ubiquinone-0
-
?
dihydroorotate + ubiquinone-10 59% of the rate with decylubiquinone Drosophila melanogaster orotate + reduced ubiquinone-10
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8
-
(S)-dihydroorotate DELTA22-37 mutant enzyme Drosophila melanogaster
5.1
-
(S)-dihydroorotate DELTA2-21 mutant enzyme Drosophila melanogaster
7.5
-
(S)-dihydroorotate wild-type recombinant enzyme Drosophila melanogaster
10.7
-
(S)-dihydroorotate DELTA2-37 mutant enzyme Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Drosophila melanogaster