Protein Variants | Comment | Organism |
---|---|---|
DELTA2-21 | instable in vivo | Drosophila melanogaster |
DELTA2-37 | instable in vivo | Drosophila melanogaster |
DELTA22-37 | instable in vivo | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
A77-1726 | 50% inhibition at 0.00045 mM, wild-type, 0.00033 mM, DELTA2-21 mutant, 0.00031 mM, DELTA22-37 mutant, 0.00036 mM, DELTA2-37 mutant | Drosophila melanogaster | |
brequinar | 50% inhibition at 0.567 mM, wild-type, 0.528 mM, DELTA2-21 mutant, 0.615 mM, DELTA22-37 mutant, 0.662 mM, DELTA2-37 mutant | Drosophila melanogaster | |
dichloroallyl lawsone | 50% inhibition at 0.00051 mM, wild-type, 0.00031 mM, DELTA2-21 mutant, 0.00020 mM, DELTA22-37 mutant, 0.00021 mM, DELTA2-37 mutant | Drosophila melanogaster | |
redoxal | 50% inhibition at 0.029 mM, wild-type, 0.057 mM, DELTA2-21 mutant, 0.040 mM, DELTA22-37 mutant, 0.056 mM, DELTA2-37 mutant | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
decylubiquinone | wild-type recombinant enzyme | Drosophila melanogaster | |
0.0113 | - |
decylubiquinone | DELTA22-37 mutant enzyme | Drosophila melanogaster | |
0.0115 | - |
decylubiquinone | DELTA2-21 mutant enzyme | Drosophila melanogaster | |
0.0118 | - |
decylubiquinone | DELTA2-37 mutant enzyme | Drosophila melanogaster | |
0.0164 | - |
dihydroorotate | DELTA2-21 mutant enzyme | Drosophila melanogaster | |
0.0166 | - |
dihydroorotate | DELTA22-37 mutant enzyme | Drosophila melanogaster | |
0.0167 | - |
dihydroorotate | DELTA2-37 mutant enzyme | Drosophila melanogaster | |
0.017 | - |
dihydroorotate | wild-type recombinant enzyme | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, His8-tag | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.6 | - |
recombinant enzyme, electron acceptor decylubiquinone | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + decylubiquinone | - |
Drosophila melanogaster | orotate + decylubiquinol | - |
r | |
dihydroorotate + 2,5-dimethylbenzoquinone | 84% of the rate with decylubiquinone | Drosophila melanogaster | orotate + reduced 2,5-dimethylbenzoquinone | - |
? | |
dihydroorotate + 2,6-dichlorophenolindophenol | 33% of the rate with decylubiquinone | Drosophila melanogaster | orotate + reduced 2,6-dichlorophenolindophenol | - |
? | |
dihydroorotate + decylplastoquinone | 78% of the rate with decylubiquinone | Drosophila melanogaster | orotate + reduced decylplastoquinone | - |
? | |
dihydroorotate + decylubiquinone | - |
Drosophila melanogaster | orotate + reduced decylubiquinone | - |
? | |
dihydroorotate + menadione | 39% of the rate with decylubiquinone | Drosophila melanogaster | orotate + reduced menadione | - |
? | |
dihydroorotate + ubiquinone-0 | 80% of the rate with decylubiquinone | Drosophila melanogaster | orotate + reduced ubiquinone-0 | - |
? | |
dihydroorotate + ubiquinone-10 | 59% of the rate with decylubiquinone | Drosophila melanogaster | orotate + reduced ubiquinone-10 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
(S)-dihydroorotate | DELTA22-37 mutant enzyme | Drosophila melanogaster | |
5.1 | - |
(S)-dihydroorotate | DELTA2-21 mutant enzyme | Drosophila melanogaster | |
7.5 | - |
(S)-dihydroorotate | wild-type recombinant enzyme | Drosophila melanogaster | |
10.7 | - |
(S)-dihydroorotate | DELTA2-37 mutant enzyme | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Drosophila melanogaster |