Application | Comment | Organism |
---|---|---|
additional information | kinetic isotope effects on flavin reduction in anaerobic stopped-flow experiments, are about 3fold for DHO labeled at the 5-position, about 4fold for DHO labeled at the 6-position, and about 6-7fold for DHO labeled at both the 5- and 6-positions, at a pH value above the pKa controlling reduction, no isotope effect was observed for DHO deuterated at the 5-position, which is consistent with a stepwise reaction, above the kinetic pKa, the deprotonation of C5 is fast enough that it does not contribute to the observed rate constant and, therefore, is not isotopically sensitive | Escherichia coli |
additional information | kinetic isotope effects on flavin reduction in anaerobic stopped-flow experiments, pKa near 9.4 controlling reduction, similar to that previously reported for the Escherichia coli enzyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + acceptor | - |
Escherichia coli | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | - |
Homo sapiens | orotate + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class 2 dihydroorotate dehydrogenases | - |
Escherichia coli |
class 2 dihydroorotate dehydrogenases | - |
Homo sapiens |
DHODH | - |
Escherichia coli |
DHODH | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11.3 | the rate constant for flavin reduction increases with pH | Homo sapiens |
6.5 | 8.5 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Escherichia coli | |
flavin | - |
Homo sapiens |