Literature summary for 1.3.5.2 extracted from

Zameitat, E.; Gojkovic, Z.; Knecht, W.; Piskur, J.; Loeffler, M.
Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans (2006), FEBS J., 273, 3183-3191.

Search for more literature for 1.3.5.2

Activating Compound

Activating Compound	Comment	Organism	Structure
oxygen	seems to promote very low DHODH activity	Candida albicans

Application

Application	Comment	Organism
medicine	background for the development of antipyrimidines with high efficacy for decreasing in situ pyrimidine nucleotide pools in Candida albicans	Candida albicans

Cloned(Commentary)

Cloned (Comment)	Organism
full-length DHODH and N-terminally truncated DHODH, which lacks the targeting sequence and the transmembrane domain recombinantly expressed in Escherichia coli BL21	Candida albicans

Protein Variants

Protein Variants	Comment	Organism
L11S	two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system	Candida albicans
L78S	two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system	Candida albicans

Inhibitors

Inhibitors	Comment	Organism	Structure
brequinar sodium	1 mM reduces the activity by more than 50% of the full-length enzyme	Candida albicans
redoxal	0.5 mM exhibit an inhibitory effect of more than 50% on full-length DHODH and N-terminally truncated DHODH compared with the noninhibited reaction	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	dihydroorotate	N-terminally truncated DHODH, fixed concentration of dihydroorotate and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM	Candida albicans
0.042	-	dihydroorotate	full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM	Candida albicans
0.065	-	dihydroorotate	N-terminally truncated DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM	Candida albicans
0.108	-	dihydroorotate	full-length DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol	Candida albicans
0.111	-	dihydroorotate	N-terminally truncated DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol	Candida albicans
0.122	-	dihydroorotate	full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM	Candida albicans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	Q874I4	-	-

Purification (Commentary)

Purification (Comment)	Organism
full-length DHODH and N-terminally truncated DHODH, by affinity chromatography	Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6	-	using decylubiquinone and 2,6-dichloroindophenol as acceptor	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone	-	Candida albicans	orotate + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone	-	?
dihydroorotate + 2,6-dichloroindophenol	-	Candida albicans	orotate + reduced 2,6-dichloroindophenol	-	?
dihydroorotate + decylubiquinone	-	Candida albicans	orotate + reduced decylubiquinone	-	?
dihydroorotate + potassium hexacyanoferrate(III)	-	Candida albicans	orotate + reduced potassium hexacyanoferrate(III)	-	?
dihydroorotate + ubiquinone 30	-	Candida albicans	orotate + reduced ubiquinone 30	-	?
dihydroorotate + ubiquinone 50	-	Candida albicans	orotate + reduced ubiquinone 50	-	?
additional information	fumarate and NAD are inadequate electron acceptors for full-length DHODH and N-terminally truncated DHODH	Candida albicans	?	-	?

Subunits

Subunits	Comment	Organism
?	48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE	Candida albicans

Synonyms

Synonyms	Comment	Organism
DHODH	-	Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1	-	dihydroorotate	full-length DHODH	Candida albicans
2.2	-	dihydroorotate	N-terminally truncated DHODH	Candida albicans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	8.0-8.5	Candida albicans

Cofactor

Cofactor	Comment	Organism	Structure
flavin	-	Candida albicans

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Release 2023.1 (January 2023)