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6.3.2.49: L-alanine-L-anticapsin ligase

This is an abbreviated version!
For detailed information about L-alanine-L-anticapsin ligase, go to the full flat file.

Word Map on EC 6.3.2.49

Reaction

ATP
+
L-alanine
 +
L-anticapsin
=
ADP
 +
phosphate
 +
bacilysin

Synonyms

alanine-anticapsin ligase, BacD, BL00235, BSU37710, EC 6.3.2.28, L-Ala-L-aniticapsin ligase, L-alanine-L-aniticapsin ligase, L-amino acid alpha-ligase, L-amino acid ligase, L-amino-acid ligase, LAL, LAL BL00235, RSp1486a, TabS, YwfE

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.49 L-alanine-L-anticapsin ligase

Cloned

Cloned on EC 6.3.2.49 - L-alanine-L-anticapsin ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
gene bacD, genetic structure of gene cluster bacABCDE, complementation study with bacD genes from several other Bacillus sources, overview
gene bacD, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
-
gene BL00235, expression of wild-type enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)
gene lal, expression of His-tagged enzyme in Escherichia coli
gene tabS, overexpression in Escherichia coli
overexpression in Escherichia coli with an N-terminal His6tag