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6.3.2.49: L-alanine-L-anticapsin ligase

This is an abbreviated version!
For detailed information about L-alanine-L-anticapsin ligase, go to the full flat file.

Word Map on EC 6.3.2.49

Reaction

ATP
+
L-alanine
 +
L-anticapsin
=
ADP
 +
phosphate
 +
bacilysin

Synonyms

alanine-anticapsin ligase, BacD, BL00235, BSU37710, EC 6.3.2.28, L-Ala-L-aniticapsin ligase, L-alanine-L-aniticapsin ligase, L-amino acid alpha-ligase, L-amino acid ligase, L-amino-acid ligase, LAL, LAL BL00235, RSp1486a, TabS, YwfE

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.49 L-alanine-L-anticapsin ligase

Purification

Purification on EC 6.3.2.49 - L-alanine-L-anticapsin ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His Trap
recombinant His-tagged enzyme from Escherichia coli
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant N-terminally GST-tagged wild-type and mutant enzymes (deletion mutant comprising residues Lys4-Tyr468) from Escherichia coli strain ArcticExpress RIL cells (DE3) by glutathione affinity chromatography, tag cleavage by PreScission protease, dialysis and removal of the tag by glutathione affinity chromatography, followed by ultrafiltration and gel filtration, recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) purified by the same procedure
recombinant wild-type enzyme and selenomethionine-labeled enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography and gel filtration