Cloned (Comment) | Organism |
---|---|
gene BL00235, expression of wild-type enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) | Bacillus licheniformis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled LAL BL00235s, sitting drop vapour diffusion method, mixing of 00.001 ml of protein solution consisting of 8 mg/ml protein, 1.6 mM ADP, 1.6 mM Met-Ala dipeptide, with 0.001 ml of reservoir solution containing 100 mM CaCl2, 24-27% w/v PEG MME 550, 4% v/v 2-propanol, and 100 mM imidazole, pH 6.5, and equilibration against 0.3 ml of reservoir solution, at room temperature, X-ray diffraction structure determination and analysis at 1.9 A and 1.6 A resolution, respectively, multi-wavelength anomalous dispersion method | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + an L-amino acid + an L-amino acid | Bacillus licheniformis | - |
ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | Bacillus licheniformis NBRC 12200 | - |
ADP + phosphate + L-aminoacyl-L-amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65D11 | gene BL00235 | - |
Bacillus licheniformis NBRC 12200 | Q65D11 | gene BL00235 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and selenomethionine-labeled enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography and gel filtration | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + an L-amino acid + an L-amino acid | - |
Bacillus licheniformis | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | the enzyme catalyzes the formation of an alpha-peptide bond between two L-amino acids in an ATP-dependent manner | Bacillus licheniformis | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | - |
Bacillus licheniformis NBRC 12200 | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | the enzyme catalyzes the formation of an alpha-peptide bond between two L-amino acids in an ATP-dependent manner | Bacillus licheniformis NBRC 12200 | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-amino-acid ligase | - |
Bacillus licheniformis |
LAL | - |
Bacillus licheniformis |
LAL BL00235 | - |
Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP-binding site structure, overview | Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the ATP-grasp superfamily | Bacillus licheniformis |
additional information | molecular basis of the substrate specificity, overview, ATP- and dipeptide-binding site structures, overview | Bacillus licheniformis |