Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + an L-amino acid | Bacillus licheniformis | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | ADP + phosphate + L-leucyl-L-amino acid | - |
? | |
ATP + L-leucine + an L-amino acid | Bacillus licheniformis NBRC12200 | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | ADP + phosphate + L-leucyl-L-amino acid | - |
? | |
ATP + L-methionine + an L-amino acid | Bacillus licheniformis | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | ADP + phosphate + L-methionyl-L-amino acid | - |
? | |
ATP + L-methionine + an L-amino acid | Bacillus licheniformis NBRC12200 | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | ADP + phosphate + L-methionyl-L-amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
gene BL00235 | - |
Bacillus licheniformis NBRC12200 | - |
gene BL00235 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-asparagine + L-methionine | - |
Bacillus licheniformis | ADP + phosphate + L-asparaginyl-L-methionine | - |
? | |
ATP + L-asparagine + L-methionine | - |
Bacillus licheniformis NBRC12200 | ADP + phosphate + L-asparaginyl-L-methionine | - |
? | |
ATP + L-leucine + an L-amino acid | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | Bacillus licheniformis | ADP + phosphate + L-leucyl-L-amino acid | - |
? | |
ATP + L-leucine + an L-amino acid | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | Bacillus licheniformis NBRC12200 | ADP + phosphate + L-leucyl-L-amino acid | - |
? | |
ATP + L-leucine + L-alanine | - |
Bacillus licheniformis | ADP + phosphate + L-leucyl-L-alanine | - |
? | |
ATP + L-leucine + L-alanine | - |
Bacillus licheniformis NBRC12200 | ADP + phosphate + L-leucyl-L-alanine | - |
? | |
ATP + L-methionine + an L-amino acid | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | Bacillus licheniformis | ADP + phosphate + L-methionyl-L-amino acid | - |
? | |
ATP + L-methionine + an L-amino acid | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is strict, only methionine or leucine are accepted as dipeptide N-terminal residues, overview | Bacillus licheniformis NBRC12200 | ADP + phosphate + L-methionyl-L-amino acid | - |
? | |
ATP + L-methionine + glycine | - |
Bacillus licheniformis | ADP + phosphate + L-methionyl-glycine | - |
? | |
ATP + L-methionine + L-alanine | best substrate combination | Bacillus licheniformis | ADP + phosphate + L-methionyl-L-alanine | - |
? | |
ATP + L-methionine + L-cysteine | - |
Bacillus licheniformis | ADP + phosphate + L-methionyl-L-cysteine | - |
? | |
ATP + L-methionine + L-leucine | - |
Bacillus licheniformis | ADP + phosphate + L-methionyl-L-leucine | - |
? | |
ATP + L-methionine + L-methionine | - |
Bacillus licheniformis | ADP + phosphate + L-methionyl-L-methionine | - |
? | |
ATP + L-serine + L-methionine | - |
Bacillus licheniformis | ADP + phosphate + L-seryl-L-methionine | - |
? | |
ATP + L-threonine + L-methionine | - |
Bacillus licheniformis | ADP + phosphate + L-threonyl-L-methionine | - |
? | |
additional information | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is very strict, overview | Bacillus licheniformis | ? | - |
? | |
additional information | the enzyme catalyzes the formation of an alpha-peptide bond in unprotected L-amino acids in an ATP-dependent manner, the substrate specificity of BL00235 is very strict, overview | Bacillus licheniformis NBRC12200 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BL00235 | - |
Bacillus licheniformis |
L-amino acid ligase | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus licheniformis |