Information on EC 6.3.2.49 - L-alanine-L-anticapsin ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.3.2.49
-
RECOMMENDED NAME
GeneOntology No.
L-alanine-L-anticapsin ligase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-alanine + L-anticapsin = ADP + phosphate + bacilysin
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bacilysin biosynthesis
-
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Biosynthesis of antibiotics
-
-
bacilysin biosynthesis
-
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SYSTEMATIC NAME
IUBMB Comments
L-alanine:L-anticapsin ligase (ADP-forming)
The enzyme, characterized from the bacterium Bacillus subtilis, is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. The enzyme requires Mg2+ or Mn2+ for activity, and has a broad substrate specificity in vitro [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
with plasmid pSB657, gene bacD
UniProt
Manually annotated by BRENDA team
with plasmid pSB657, gene bacD
UniProt
Manually annotated by BRENDA team
gene BL00235
UniProt
Manually annotated by BRENDA team
strain NBRC12200, gene BL00235
-
-
Manually annotated by BRENDA team
gene bacD
-
-
Manually annotated by BRENDA team
gene bacD
-
-
Manually annotated by BRENDA team
with plasmid pSB657, gene bacD
UniProt
Manually annotated by BRENDA team
NBRC3134
UniProt
Manually annotated by BRENDA team
no activity in Bacillus amyloliquefaciens
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus amyloliquefaciens GSB272
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus coagulans
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis ATCC9789
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus megaterium
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus megaterium PV361
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus pumilus
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus pumilus ATCC12140
-
-
-
Manually annotated by BRENDA team
gene tabS
UniProt
Manually annotated by BRENDA team
gene tabS
UniProt
Manually annotated by BRENDA team
strain JCM 10489, gene lal
UniProt
Manually annotated by BRENDA team
strain JCM 10489, gene lal
UniProt
Manually annotated by BRENDA team
-
Q97RD7
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
show the reaction diagram
ATP + 2 L-cysteine
ADP + phosphate + L-cysteinyl-L-cysteine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + alanine + alanine
ADP + phosphate + L-alanyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
show the reaction diagram
ATP + arginine + histidine
ADP + phosphate + L-arginyl-L-histidine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + arginine + methionine
ADP + phosphate + L-arginyl-L-methionine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + arginine + phenylalanine
ADP + phosphate + L-arginyl-L-phenylalanine
show the reaction diagram
ATP + arginine + tryptophan
ADP + phosphate + L-arginyl-L-tryptophan
show the reaction diagram
ATP + arginine + tyrosine
ADP + phosphate + L-arginyl-L-tyrosine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + beta-alanine + beta-alanine
ADP + phosphate + beta-alanyl-beta-alanine
show the reaction diagram
-
-
-
?
ATP + cis-4-hydroxy-L-proline + L-threonine
ADP + phosphate + cis-4-hydroxy-L-prolyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + cysteine + alanine
ADP + phosphate + L-cysteinyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + cysteine + glutamic acid
ADP + phosphate + ?
show the reaction diagram
ATP + cysteine + serine
ADP + phosphate + L-cysteinyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + cysteine + threonine
ADP + phosphate + L-cysteinyl-L-threonine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + glutamic acid + leucine
ADP + phosphate + ?
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + glutamine + glutamic acid
ADP + phosphate + ?
show the reaction diagram
assay at pH 8.0, 37C
-
-
?
ATP + glutamine + phenylalanine
ADP + phosphate + L-glutaminyl-L-phenylalanine
show the reaction diagram
assay at pH 8.0, 37C
-
-
?
ATP + glycine + L-proline
ADP + phosphate + glycyl-L-proline
show the reaction diagram
assay at pH 8.0, 37C
-
-
?
ATP + histidine + alanine
ADP + phosphate + L-histidyl-L-alanine
show the reaction diagram
ATP + histidine + asparagine
ADP + phosphate + L-histidyl-L-asparagine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + histidine + cysteine
ADP + phosphate + L-histidyl-L-cysteine
show the reaction diagram
ATP + histidine + glutamine
ADP + phosphate + L-histidyl-L-glutamine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + histidine + histidine
ADP + phosphate + L-histidyl-L-histidine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + histidine + methionine
ADP + phosphate + L-histidyl-L-methionine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + histidine + phenylalanine
ADP + phosphate + L-histidyl-L-phenylalanine
show the reaction diagram
ATP + histidine + proline
ADP + phosphate + L-histidyl-L-proline
show the reaction diagram
ATP + histidine + serine
ADP + phosphate + L-histidyl-L-serine
show the reaction diagram
ATP + histidine + tryptophan
ADP + phosphate + L-histidyl-L-tryptophan
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + histidine + valine
ADP + phosphate + L-histidyl-L-valine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + isoleucine + alanine
ADP + phosphate + L-isoleucyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + isoleucine + cysteine
ADP + phosphate + L-isoleucyl-L-cysteine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + isoleucine + serine
ADP + phosphate + L-isoleucyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + L-alanine + (1R,2S,5R,6S)-dihydroanticapsin
ADP + phosphate + dihydrobacilysin
show the reaction diagram
ATP + L-alanine + (1R,2S,6R)-anticapsin
ADP + phosphate + bacilysin
show the reaction diagram
ATP + L-alanine + 3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine
ADP + phosphate + L-alanyl-[3-[(1S)-4-oxocyclohex-2-en-1-yl]-L-alanine]
show the reaction diagram
ATP + L-alanine + L-anticapsin
ADP + phosphate + bacilysin
show the reaction diagram
ATP + L-alanine + L-glutamine
ADP + phosphate + L-alanyl-L-glutamine
show the reaction diagram
ATP + L-alanine + L-phenylalanine
ADP + phosphate + L-alanyl-L-phenylalanine
show the reaction diagram
ATP + L-arginine + beta-alanine
ADP + phosphate + beta-alanyl-L-arginine
show the reaction diagram
-
-
-
?
ATP + L-arginine + L-phenylalanine
ADP + phosphate + L-arginyl-L-phenylalanine
show the reaction diagram
-
-
-
?
ATP + L-asparagine + L-glutamine
ADP + phosphate + L-asparaginyl-L-glutamine
show the reaction diagram
-
-
-
?
ATP + L-asparagine + L-isoleucine
ADP + phosphate + L-asparaginyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-asparagine + L-leucine
ADP + phosphate + L-asparaginyl-L-leucine
show the reaction diagram
ATP + L-asparagine + L-methionine
ADP + phosphate + L-asparaginyl-L-methionine
show the reaction diagram
ATP + L-asparagine + L-phenylalanine
ADP + phosphate + L-asparaginyl-L-phenylalanine
show the reaction diagram
ATP + L-asparagine + L-serine
ADP + phosphate + L-asparaginyl-L-serine
show the reaction diagram
-
-
-
?
ATP + L-asparagine + L-threonine
ADP + phosphate + L-asparaginyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-asparagine + L-tryptophan
ADP + phosphate + L-asparaginyl-L-tryptophan
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + L-asparagine + L-valine
ADP + phosphate + L-asparaginyl-L-valine
show the reaction diagram
-
-
-
?
ATP + L-aspartate + L-alanine
ADP + phosphate + L-aspartyl-L-alanine
show the reaction diagram
-
-
-
?
ATP + L-aspartate + L-isoleucine
ADP + phosphate + L-aspartyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-aspartate + L-leucine
ADP + phosphate + L-aspartyl-L-leucine
show the reaction diagram
-
-
-
?
ATP + L-aspartate + L-methionine
ADP + phosphate + L-aspartyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-azetidine-2-carboxylate + L-threonine
ADP + phosphate + L-azetidine-2-carboxyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-cysteine + glycine
ADP + phosphate + L-cysteinyl-glycine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + L-glutamate + beta-alanine
ADP + phosphate + beta-alanyl-L-glutamine
show the reaction diagram
-
-
-
?
ATP + L-glutamate + L-methionine
ADP + phosphate + L-glutamyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-glutamine + L-alanine
ADP + phosphate + L-glutaminyl-L-alanine
show the reaction diagram
ATP + L-glutamine + L-arginine
ADP + phosphate + L-glutaminyl-L-arginine
show the reaction diagram
-
-
-
?
ATP + L-glutamine + L-histidine
ADP + phosphate + L-glutaminyl-L-histidine
show the reaction diagram
-
-
-
?
ATP + L-glutamine + L-isoleucine
ADP + phosphate + L-glutaminyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-glutamine + L-leucine
ADP + phosphate + L-glutaminyl-L-leucine
show the reaction diagram
ATP + L-glutamine + L-methionine
ADP + phosphate + L-glutaminyl-L-methionine
show the reaction diagram
ATP + L-glutamine + L-phenylalanine
ADP + phosphate + L-glutaminyl-L-phenylalanine
show the reaction diagram
ATP + L-glutamine + L-serine
ADP + phosphate + L-glutaminyl-L-serine
show the reaction diagram
-
-
-
?
ATP + L-glutamine + L-threonine
ADP + phosphate + L-glutaminyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-glutamine + L-tryptophan
ADP + phosphate + L-glutaminyl-L-tryptophan
show the reaction diagram
ATP + L-glutamine + L-valine
ADP + phosphate + L-glutaminyl-L-valine
show the reaction diagram
-
-
-
?
ATP + L-histidine + glycine
ADP + phosphate + L-histidyl-glycine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + L-isoleucine + beta-alanine
ADP + phosphate + beta-alanyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-leucine + an L-amino acid
ADP + phosphate + L-leucyl-L-amino acid
show the reaction diagram
ATP + L-leucine + beta-alanine
ADP + phosphate + beta-alanyl-L-leucine
show the reaction diagram
-
-
-
?
ATP + L-leucine + glycine
ADP + phosphate + L-leucyl-glycine
show the reaction diagram
ATP + L-leucine + L-alanine
ADP + phosphate + L-leucyl-L-alanine
show the reaction diagram
ATP + L-leucine + L-isoleucine
ADP + phosphate + L-leucyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-leucine + L-serine
ADP + phosphate + L-leucyl-L-serine
show the reaction diagram
-
-
-
?
ATP + L-methionine + an L-amino acid
ADP + phosphate + L-methionyl-L-amino acid
show the reaction diagram
ATP + L-methionine + beta-alanine
ADP + phosphate + beta-alanyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-methionine + glycine
ADP + phosphate + L-methionyl-glycine
show the reaction diagram
ATP + L-methionine + L-alanine
ADP + phosphate + L-methionyl-L-alanine
show the reaction diagram
-
best substrate combination
-
-
?
ATP + L-methionine + L-cysteine
ADP + phosphate + L-methionyl-L-cysteine
show the reaction diagram
-
-
-
-
?
ATP + L-methionine + L-leucine
ADP + phosphate + L-methionyl-L-leucine
show the reaction diagram
-
-
-
-
?
ATP + L-methionine + L-methionine
ADP + phosphate + L-methionyl-L-methionine
show the reaction diagram
-
-
-
-
?
ATP + L-phenylalanine + glycine
ADP + phosphate + L-phenylalanyl-glycine
show the reaction diagram
ATP + L-phenylalanine + glycine
ADP + phosphate + L-phenylalanylglycine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-alanine
ADP + phosphate + L-phenylalanyl-L-alanine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-arginine
ADP + phosphate + L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-asparagine
ADP + phosphate + L-phenylalanyl-L-asparagine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-glutamine
ADP + phosphate + L-phenylalanyl-L-glutamine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-histidine
ADP + phosphate + L-phenylalanyl-L-histidine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-isoleucine
ADP + phosphate + L-phenylalanyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-leucine
ADP + phosphate + L-phenylalanyl-L-leucine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-lysine
ADP + phosphate + L-phenylalanyl-L-lysine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-methionine
ADP + phosphate + L-phenylalanyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-phenylalanine
ADP + phosphate + L-phenylalanyl-L-phenylalanine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-proline
ADP + phosphate + L-phenylalanyl-L-proline
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-serine
ADP + phosphate + L-phenylalanyl-L-serine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-threonine
ADP + phosphate + L-phenylalanyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-tryptophan
ADP + phosphate + L-phenylalanyl-L-tryptophan
show the reaction diagram
-
-
-
?
ATP + L-phenylalanine + L-valine
ADP + phosphate + L-phenylalanyl-L-valine
show the reaction diagram
-
-
-
?
ATP + L-pipecolic acid + L-threonine
ADP + phosphate + L-pipecoloyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-proline + beta-alanine
ADP + phosphate + beta-alanyl-L-proline
show the reaction diagram
-
-
-
?
ATP + L-proline + glycine
ADP + phosphate + L-prolylglycine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-alanine
ADP + phosphate + L-prolyl-L-alanine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-asparagine
ADP + phosphate + L-prolyl-L-asparagine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-aspartate
ADP + phosphate + L-prolyl-L-aspartate
show the reaction diagram
-
-
-
?
ATP + L-proline + L-glutamate
ADP + phosphate + L-prolyl-L-glutamate
show the reaction diagram
-
-
-
?
ATP + L-proline + L-glutamine
ADP + phosphate + L-prolyl-L-glutamine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-isoleucine
ADP + phosphate + L-prolyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-leucine
ADP + phosphate + L-prolyl-L-leucine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-methionine
ADP + phosphate + L-prolyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-serine
ADP + phosphate + L-prolyl-L-serine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-threonine
ADP + phosphate + L-prolyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-proline + L-valine
ADP + phosphate + L-prolyl-L-valine
show the reaction diagram
-
-
-
?
ATP + L-serine + L-methionine
ADP + phosphate + L-seryl-L-methionine
show the reaction diagram
-
-
-
-
?
ATP + L-threonine + beta-alanine
ADP + phosphate + beta-alanyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-threonine + L-methionine
ADP + phosphate + L-threonyl-L-methionine
show the reaction diagram
-
-
-
-
?
ATP + L-tryptophan + beta-alanine
ADP + phosphate + beta-alanyl-L-tryptophan
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + glycine
ADP + phosphate + L-tryptophanyl-glycine
show the reaction diagram
ATP + L-tryptophan + glycine
ADP + phosphate + L-tryptophylglycine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-alanine
ADP + phosphate + L-tryptophyl-L-alanine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-arginine
ADP + phosphate + L-tryptophyl-L-arginine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-asparagine
ADP + phosphate + L-tryptophyl-L-asparagine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-aspartate
ADP + phosphate + L-tryptophyl-L-aspartate
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-glutamate
ADP + phosphate + L-tryptophyl-L-glutamate
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-glutamine
ADP + phosphate + L-tryptophyl-L-glutamine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-histidine
ADP + phosphate + L-tryptophyl-L-histidine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-isoleucine
ADP + phosphate + L-tryptophyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-leucine
ADP + phosphate + L-tryptophyl-L-leucine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-lysine
ADP + phosphate + L-tryptophyl-L-lysine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-methionine
ADP + phosphate + L-tryptophyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-proline
ADP + phosphate + L-tryptophyl-L-proline
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-serine
ADP + phosphate + L-tryptophyl-L-serine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-threonine
ADP + phosphate + L-tryptophyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-tryptophan
ADP + phosphate + L-tryptophyl-L-tryptophan
show the reaction diagram
-
-
-
?
ATP + L-tryptophan + L-valine
ADP + phosphate + L-tryptophyl-L-valine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + glycine
ADP + phosphate + L-tyrosinyl-glycine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + L-tyrosine + L-arginine
ADP + phosphate + L-tyrosyl-L-arginine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-glutamine
ADP + phosphate + L-tyrosyl-L-glutamine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-histidine
ADP + phosphate + L-tyrosyl-L-histidine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-isoleucine
ADP + phosphate + L-tyrosyl-L-isoleucine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-methionine
ADP + phosphate + L-tyrosyl-L-methionine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-phenylalanine
ADP + phosphate + L-tyrosyl-L-phenylalanine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-proline
ADP + phosphate + L-tyrosyl-L-proline
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-threonine
ADP + phosphate + L-tyrosyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-tryptophan
ADP + phosphate + L-tyrosyl-L-tryptophan
show the reaction diagram
-
-
-
?
ATP + L-tyrosine + L-valine
ADP + phosphate + L-tyrosyl-L-valine
show the reaction diagram
-
-
-
?
ATP + L-valine + beta-alanine
ADP + phosphate + beta-alanyl-L-valine
show the reaction diagram
-
-
-
?
ATP + leucine + alanine
ADP + phosphate + L-leucyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + leucine + cysteine
ADP + phosphate + L-leucyl-L-cysteine
show the reaction diagram
ATP + leucine + serine
ADP + phosphate + L-leucyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + leucine + threonine
ADP + phosphate + L-leucyl-L-threonine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + lysine + histidine
ADP + phosphate + L-lysyl-L-histidine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + lysine + phenylalanine
ADP + phosphate + L-lysyl-L-phenylalanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + alanine
ADP + phosphate + L-methionyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + cysteine
ADP + phosphate + L-methionyl-L-cysteine
show the reaction diagram
ATP + methionine + isoleucine
ADP + phosphate + L-methionyl-L-isoleucine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + leucine
ADP + phosphate + L-methionyl-L-leucine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + methionine
ADP + phosphate + L-methionyl-L-methionine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + serine
ADP + phosphate + L-methionyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + threonine
ADP + phosphate + L-methionyl-L-threonine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + methionine + valine
ADP + phosphate + L-methionyl-L-valine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + phenylalanine + alanine
ADP + phosphate + L-phenylalanyl-L-alanine
show the reaction diagram
ATP + phenylalanine + cysteine
ADP + phosphate + L-phenylalanyl-L-cysteine
show the reaction diagram
ATP + phenylalanine + isoleucine
ADP + phosphate + L-phenylalanyl-L-isoleucine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + phenylalanine + methionine
ADP + phosphate + L-phenylalanyl-L-methionine
show the reaction diagram
ATP + phenylalanine + phenylalanine
ADP + phosphate + L-phenylalanyl-L-phenylalanine
show the reaction diagram
ATP + phenylalanine + serine
ADP + phosphate + L-phenylalanyl-L-serine
show the reaction diagram
ATP + phenylalanine + threonine
ADP + phosphate + L-phenylalanyl-L-threonine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + phenylalanine + valine
ADP + phosphate + L-phenylalanyl-L-valine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + serine + alanine
ADP + phosphate + L-serinyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + serine + serine
ADP + phosphate + L-serinyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + trans-4-hydroxy-L-proline + L-threonine
ADP + phosphate + trans-4-hydroxy-L-prolyl-L-threonine
show the reaction diagram
-
-
-
?
ATP + tryptophan + alanine
ADP + phosphate + L-tryptophanyl-L-alanine
show the reaction diagram
ATP + tryptophan + cysteine
ADP + phosphate + L-tryptophanyl-L-cysteine
show the reaction diagram
ATP + tryptophan + isoleucine
ADP + phosphate + L-tryptophanyl-L-isoleucine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tryptophan + leucine
ADP + phosphate + L-tryptophanyl-L-leucine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tryptophan + methionine
ADP + phosphate + L-tryptophanyl-L-methionine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tryptophan + phenylalanine
ADP + phosphate + L-tryptophanyl-L-phenylalanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tryptophan + serine
ADP + phosphate + L-tryptophanyl-L-serine
show the reaction diagram
ATP + tryptophan + threonine
ADP + phosphate + L-tryptophanyl-L-threonine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tryptophan + tryptophan
ADP + phosphate + L-tryptophanyl-L-tryptophan
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tryptophan + valine
ADP + phosphate + L-tryptophanyl-L-valine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tyrosine + alanine
ADP + phosphate + L-tyrosinyl-L-alanine
show the reaction diagram
ATP + tyrosine + cysteine
ADP + phosphate + L-tyrosinyl-L-cysteine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tyrosine + methionine
ADP + phosphate + L-tyrosinyl-L-methionine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tyrosine + phenylalanine
ADP + phosphate + L-tyrosinyl-L-phenylalanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + tyrosine + serine
ADP + phosphate + L-tyrosinyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + valine + alanine
ADP + phosphate + L-valinyl-L-alanine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
ATP + valine + serine
ADP + phosphate + L-valinyl-L-serine
show the reaction diagram
-
assay at pH 8.0, 37C
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + an L-amino acid + an L-amino acid
ADP + phosphate + L-aminoacyl-L-amino acid
show the reaction diagram
ATP + L-alanine + (1R,2S,6R)-anticapsin
ADP + phosphate + bacilysin
show the reaction diagram
ATP + L-alanine + L-anticapsin
ADP + phosphate + bacilysin
show the reaction diagram
ATP + L-leucine + an L-amino acid
ADP + phosphate + L-leucyl-L-amino acid
show the reaction diagram
ATP + L-methionine + an L-amino acid
ADP + phosphate + L-methionyl-L-amino acid
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
Mg2+ or Mn2+ is essential for activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid
-
competitive inhibition for L-alanine but not for L-phenylalanine
-
CdSO4
-
2 mM, complete inhibition
ZnSO4
-
2 mM, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.42
ATP
-
pH 9.0, 37C
1.5 - 105
L-alanine
0.07
L-glutamine
-
pH 9.0, 37C
16 - 92
L-phenylalanine
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.2 - 10.8
L-alanine
7.2 - 10.8
L-phenylalanine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0154
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid
-
pH 7.7, 37C
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
activity range, profile overview
8.5 - 10.5
-
pH 8.5: about 70% of maximal activity, pH 10.5: about 35% of maximal activity
9 - 9.5
enzyme activity decreased sharply above pH 10.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
activity range, profile overview
20 - 50
significant decrease in activity above 45C
25 - 45
-
25C: about 70% of maximal activity, 45C: about 55% of maximal activity
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49500
x * 49500, recombinant His-tagged enzyme, SDS-PAGE
53000
-
x * 53000, SDS-PAGE
55000
-
2 * 55000, SDS-PAGE
105000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type and selenomethionine-labeled LAL BL00235s, sitting drop vapour diffusion method, mixing of 00.001 ml of protein solution consisting of 8 mg/ml protein, 1.6 mM ADP, 1.6 mM Met-Ala dipeptide, with 0.001 ml of reservoir solution containing 100 mM CaCl2, 24-27% w/v PEG MME 550, 4% v/v 2-propanol, and 100 mM imidazole, pH 6.5, and equilibration against 0.3 ml of reservoir solution, at room temperature, X-ray diffraction structure determination and analysis at 1.9 A and 1.6 A resolution, respectively, multi-wavelength anomalous dispersion method
crystal structure of the enzyme in complex with ADP and an intermediate analog, phosphorylated phosphinate L-alanyl-L-phenylalanine, refined to 2.5 A resolution, sitting-drop vapor-diffusion method at 20C
-
purified wild-type and selenomethionine-labeled YwfE in complex with ADP, MgCl2 and dipeptide L-Ala-L-Gln, hanging drop vapour diffusion method, mixing of 0.002 ml of protein solution containing 8 mg/ml protein, 10 mM ADP, 10 mM MgCl2, 100 mM L-Ala-L-Gln with 0.002 ml reservoir solution, equilibration against 0.5 ml of reservoir solution, the deletion-mutant crystals are grown in reservoir solution consisting of 17% w/v PEG 3350, 0.3 M NaCl, 0.1 M MES-NaOH, pH 6.75, 5% v/v ethylene glycol, 1 mM DTT, X-ray diffraction structure determination and analysis at 1.9 A and 2.8 A, respectively
-
YwfE with bound ADP-Mg2+-phosphate and ADP-Mg2+-L-Ala, X-ray diffraction structure determination and analysis at 1.9 and 2.0 A resolution, respectively
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His Trap
recombinant His-tagged enzyme from Escherichia coli
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant N-terminally GST-tagged wild-type and mutant enzymes (deletion mutant comprising residues Lys4-Tyr468) from Escherichia coli strain ArcticExpress RIL cells (DE3) by glutathione affinity chromatography, tag cleavage by PreScission protease, dialysis and removal of the tag by glutathione affinity chromatography, followed by ultrafiltration and gel filtration, recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) purified by the same procedure
-
recombinant wild-type enzyme and selenomethionine-labeled enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322; gene bacD, genetic structure of gene cluster bacABCDE, complementation study with bacD genes from several other Bacillus sources, overview
gene bacD, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
-
gene BL00235, expression of wild-type enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)
gene lal, expression of His-tagged enzyme in Escherichia coli
gene tabS, overexpression in Escherichia coli
overexpression in Escherichia coli with an N-terminal His6tag
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S1845A
-
no significant difference in kcat value between the wild type and the mutant enzyme. 2fold increase in Km-value for L-alanine
Y332A
-
site-directed mutagenesis, mutation of Trp332 to Ala causes the enzyme to hydrolyze ATP, even in the absence of L-Ala, and the structure of this mutant protein show a cavity in the N-terminal substrate-binding pocket
Y75F
-
no significant difference in kcat value between the wild type and the mutant enzyme, 2fold increase in KM-value for L-phenylalanine
S1845A
-
no significant difference in kcat value between the wild type and the mutant enzyme. 2fold increase in Km-value for L-alanine
-
Y75F
-
no significant difference in kcat value between the wild type and the mutant enzyme, 2fold increase in KM-value for L-phenylalanine
-
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
synthesis
Show AA Sequence (146 entries)
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