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6.1.1.7: alanine-tRNA ligase

This is an abbreviated version!
For detailed information about alanine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.7

Reaction

ATP
+
L-alanine
 +
tRNAAla
=
AMP
 +
diphosphate
 +
L-alanyl-tRNAAla

Synonyms

AARS2, Ala-tRNA synthetase, ALA1, ALA2, Alanine transfer RNA synthetase, Alanine translase, Alanine tRNA synthetase, Alanine--tRNA ligase, Alanine-transfer RNA ligase, alanine-tRNA ligase, alanyl tRNA ligase, Alanyl-transfer ribonucleate synthetase, Alanyl-transfer ribonucleic acid synthetase, Alanyl-transfer RNA synthetase, alanyl-tRNA ligase, alanyl-tRNA synthase, Alanyl-tRNA synthetase, alanyltRNA synthetase, AlaRS, mitochondrial alanyl-tRNA synthetase, More, mtAlaRS, MurM, MurN, Synthase, alanyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.7 alanine-tRNA ligase

Purification

Purification on EC 6.1.1.7 - alanine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography, and gel filtration
-
nickel-chelating Sepharose resin column chromatography
-
partial, from a multienzyme complex containing at least 16 aminoacyl-tRNA synthetase activities
-
purified by Ni-NTA chromatography and SDS gel electrophoresis
-
recombinant full-length AlaRS and AlaRS domains from Escherichia coli strain BL21 by anion exchange chromatography followed by hydrophobic interaction and adsorption chromatography, respectively
-
recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged full-length enzyme and C-terminal domain C-Ala from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, followed by tag cleavage through TEV protease, tag and TEV protease are removed by another turn of nickel affinity chromatography, and ultrafiltration and dialysis
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis
recombinant PH0574, from Escherichia coli strain BL21 by two steps of anion exchange chromatography, hydroxyapatite chromatography, and gel filtration
Superose 12 column chromatography, Q Sepharose column chromatography and Sephacryl S200 gel filtration
-
using Ni-Sepharose columns and anion-exchange chromatography. The yield of purified protein is 4-5 mg of protein per litre of culture, judged to be above 95% purity by SDS-PAGE
-