6.1.1.7: alanine-tRNA ligase
This is an abbreviated version!
For detailed information about alanine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.7
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6.1.1.7
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aminoacylation
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synthetases
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aminoacyl-trna
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leukodystrophy
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alanylation
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mischarged
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aarss
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leukoencephalopathy
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mistranslation
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tmrnas
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charcot-marie-tooth
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noncognate
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thrrs
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threonyl-trna
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minihelix
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seryl-trna
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anti-pl-12
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valrs
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misactivation
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microhelix
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ilers
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trans-translation
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molecular biology
- 6.1.1.7
- aminoacylation
- synthetases
- aminoacyl-trna
- leukodystrophy
-
alanylation
-
mischarged
-
aarss
- leukoencephalopathy
-
mistranslation
- tmrnas
- charcot-marie-tooth
-
noncognate
- thrrs
- threonyl-trna
- minihelix
- seryl-trna
-
anti-pl-12
- valrs
-
misactivation
- microhelix
- ilers
-
trans-translation
- molecular biology
Reaction
Synonyms
AARS2, Ala-tRNA synthetase, ALA1, ALA2, Alanine transfer RNA synthetase, Alanine translase, Alanine tRNA synthetase, Alanine--tRNA ligase, Alanine-transfer RNA ligase, alanine-tRNA ligase, alanyl tRNA ligase, Alanyl-transfer ribonucleate synthetase, Alanyl-transfer ribonucleic acid synthetase, Alanyl-transfer RNA synthetase, alanyl-tRNA ligase, alanyl-tRNA synthase, Alanyl-tRNA synthetase, alanyltRNA synthetase, AlaRS, mitochondrial alanyl-tRNA synthetase, More, mtAlaRS, MurM, MurN, Synthase, alanyl-transfer ribonucleate
ECTree
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KCat KM Value
KCat KM Value on EC 6.1.1.7 - alanine-tRNA ligase
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additional information
additional information
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the wild-type AlaRS editing domain deacylates Ser-tRNAAla with a kcat/Km of 66 mM/s, equivalent to a rate enhancement of 6000 over the rate of enzyme-independent deacylation but only 12.2fold greater than the rate with Ala-tRNAAla. While the E664A and T567G substitutions only minimally decrease kcat/Km, Q584H, I667E, and C666A AlaRS are more compromised in activity, with decreases in kcat/Km in the range of 6fold, 6.6fold, and 15fold. C666A AlaRS is 1.7fold more active on Ala-tRNAAla relative to Ser-tRNAAl. Deacylation rates of Ser-tRNAAla and Ala-tRNAAla in the absence of enzyme are determined by fitting the progress curves to equations describing a first-order decay
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