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6.1.1.7: alanine-tRNA ligase

This is an abbreviated version!
For detailed information about alanine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.7

Reaction

ATP
+
L-alanine
 +
tRNAAla
=
AMP
 +
diphosphate
 +
L-alanyl-tRNAAla

Synonyms

AARS2, Ala-tRNA synthetase, ALA1, ALA2, Alanine transfer RNA synthetase, Alanine translase, Alanine tRNA synthetase, Alanine--tRNA ligase, Alanine-transfer RNA ligase, alanine-tRNA ligase, alanyl tRNA ligase, Alanyl-transfer ribonucleate synthetase, Alanyl-transfer ribonucleic acid synthetase, Alanyl-transfer RNA synthetase, alanyl-tRNA ligase, alanyl-tRNA synthase, Alanyl-tRNA synthetase, alanyltRNA synthetase, AlaRS, mitochondrial alanyl-tRNA synthetase, More, mtAlaRS, MurM, MurN, Synthase, alanyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.7 alanine-tRNA ligase

Cloned

Cloned on EC 6.1.1.7 - alanine-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
deletion mutants with reduced aminoacylation efficiency, expressed in Escherichia coli strain BL21
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(deltaDE3) cells
-
expressed in Escherichia coli BL21DE3star cells
-
expressed in HEK-293T cells
expression in Escherichia coli
expression in Escherichia coli strain BL21
expression in Pichia sp.
-
expression of the full-length AlaRS, of the N-terminal fragment lacking the C-terminal oligomerization domain, residues 1-739, and of the C-terminal oligomerization domain of AlaRS, residues 737-906, in Escherichia coli strain BL21
-
gene ALA1, DNA and amino acid sequence determination and analysis, mitochondrial and cytoplasmic isozymes are encoded by a single nuclear gene ALA1, through alternative use of inframe successive ACG triplets and a downstream AUG triplet, overview, despite participation of the non-AUG-initiated leader peptide in mitochondrial localization, the leader peptide per se cannot target a cytoplasmic passenger protein into mitochondria under normal conditions, functional mapping
-
gene ALA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes
gene ALA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes, recombinant expression of GFP-tagged isozyme ALA1 in Saccharomyces cerevisiae strain INVSc1
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes, recombinant expression of GFP-tagged isozyme ALA2 in Saccharomyces cerevisiae strain INVSc1
gene alaS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His6-tagged full-length enzyme and C-terminal domain C-Ala in Escherichia coli strain BL21(DE3)
gene alaS, DNA sequence determination and analysis, phylogenetic origin
gene alaS, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene alaS, sequence comparisons, recombinant expression of a C-Ala construct consisting of the C-terminal 757-968 amino acids
gene cdc64, DNA sequence determination and analysis, gene maps to a locus between met7 and prt1 on chromosome XV, gene derepresses gcn4 expression, the cdc64-1 mutant strain can be complemented by expression of the wild-type enzyme
-
gene PH0574, expression in Escherichia coli strain BL21
recombinant MurN is expressed and purified as MBP fusion protein in Escherichia coli
-
temperature-sensitve mutant tsET12