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5.4.99.13: isobutyryl-CoA mutase

This is an abbreviated version!
For detailed information about isobutyryl-CoA mutase, go to the full flat file.

Word Map on EC 5.4.99.13

Reaction

2-methylpropanoyl-CoA
=
butanoyl-CoA

Synonyms

adenosylcobalamin-dependent isobutyryl-CoA mutase, AdoCbl-dependent PCM, butyryl-CoA:isobutyryl-CoA mutase, CmIcmF, ICM, IcmF, isobutyryl coenzyme A mutase, isobutyryl-CoA mutase fused, isovaleryl-CoA/pivalyl-CoA mutase, PCM, pivalyl-CoA mutase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.13 isobutyryl-CoA mutase

Molecular Weight

Molecular Weight on EC 5.4.99.13 - isobutyryl-CoA mutase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
135000
recombinant detagged enzyme, large subunit dimer, gel filtration
14300
alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain
153000
gel filtration
20000
recombinant detagged enzyme, small subunit monomer, gel filtration
62500
alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain
65000
-
IcmA, 1 * 65000 + several proteins of lower mass, SDS-PAGE
additional information
size exclusion chromatography yields an estimated molecular mass of 135 kDa, consistent with the large subunit being a homodimer. The recombinant small subunit of PCM is purified as N-terminally His6-tagged protein and elutes with an apparent mass of 20 kDa in gel filtration, suggesting that it exists as a monomer based on the predicted mass of the polypeptide of 17.7 kDa. Gel filtration of a 1:1 mixture of the large and small subunits ofthe enzyme in presence of 5'-deoxyadenosylcobalamin, shows no evidence of complex formation, indicating weak interaction between the subunits under these conditions