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5.4.99.13: isobutyryl-CoA mutase

This is an abbreviated version!
For detailed information about isobutyryl-CoA mutase, go to the full flat file.

Word Map on EC 5.4.99.13

Reaction

2-methylpropanoyl-CoA
=
butanoyl-CoA

Synonyms

adenosylcobalamin-dependent isobutyryl-CoA mutase, AdoCbl-dependent PCM, butyryl-CoA:isobutyryl-CoA mutase, CmIcmF, ICM, IcmF, isobutyryl coenzyme A mutase, isobutyryl-CoA mutase fused, isovaleryl-CoA/pivalyl-CoA mutase, PCM, pivalyl-CoA mutase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.13 isobutyryl-CoA mutase

Crystallization

Crystallization on EC 5.4.99.13 - isobutyryl-CoA mutase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified enzyme in complex with four different substrates, GDP and isovaleryl-CoA, or isobutyryl-CoA or n-butytryl-CoA or pivalyl-CoA, hanging drop vapor diffusion technique, mixing of 0.001 ml of 11.7 mg/ml IcmF protein in 100 mM NaCl, 50 mM HEPES, pH 7.5, 1 mM GDP, 3 mM MgCl2, 0.3 mM AdoCbl, with 0.001 ml of reservoir solution containing 0.7-0.75 M potassium sodium tartrate, 0.2 M ammonium acetate, 0.1 M imidazole, pH 7.0-7.7, and 3% v/v ethylene glycol, and equilibration against 0.5 ml reservoir solution, 3-6 weeks, at 25°C, X-ray diffraction structure determination and analysis at 3.4-3.5 A resolution