5.4.99.13: isobutyryl-CoA mutase
This is an abbreviated version!
For detailed information about isobutyryl-CoA mutase, go to the full flat file.
Word Map on EC 5.4.99.13
-
5.4.99.13
-
methylmalonyl-coa
-
b12-dependent
-
mutases
-
acyl-coas
-
n-butyryl-coa
-
cinnamonensis
-
icmfs
-
polyketide
-
succinyl-coa
-
monensin
-
isomerases
-
meaa
-
5'-deoxyadenosylcobalamin-dependent
-
2-hydroxyisobutyrate
-
extorquens
-
isobutyrate
-
cobiialamin
-
2r-methylmalonyl-coa
-
synthesis
-
b12-binding
-
mutase-catalyzed
-
5'-deoxyadenosine
-
5\'-deoxyadenosyl
-
coa-dependent
-
isobutanol
-
methylmalonyl
-
methylobacterium
-
crotonyl-coa
-
pivalic
-
biofuel production
- 5.4.99.13
- methylmalonyl-coa
-
b12-dependent
- mutases
- acyl-coas
- n-butyryl-coa
- cinnamonensis
-
icmfs
- polyketide
- succinyl-coa
- monensin
- isomerases
- meaa
-
5'-deoxyadenosylcobalamin-dependent
- 2-hydroxyisobutyrate
- extorquens
- isobutyrate
-
cobiialamin
-
2r-methylmalonyl-coa
- synthesis
-
b12-binding
-
mutase-catalyzed
- 5'-deoxyadenosine
-
5\'-deoxyadenosyl
-
coa-dependent
- isobutanol
-
methylmalonyl
- methylobacterium
- crotonyl-coa
-
pivalic
- biofuel production
Reaction
Synonyms
adenosylcobalamin-dependent isobutyryl-CoA mutase, AdoCbl-dependent PCM, butyryl-CoA:isobutyryl-CoA mutase, CmIcmF, ICM, IcmF, isobutyryl coenzyme A mutase, isobutyryl-CoA mutase fused, isovaleryl-CoA/pivalyl-CoA mutase, PCM, pivalyl-CoA mutase
ECTree
5.4.99.13 isobutyryl-CoA mutaseAdvanced search results
results (
results (Purification
Purification on EC 5.4.99.13 - isobutyryl-CoA mutase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration
partial, using ammonium sulfate precipitation, and DEAE-Sepharose column chromatography
-
recombinant His-MBP-tagged wild-type enzyme and His6-SUMO-tagged mutant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, tag cleavage by TEV protease, dialysis, amylose affinity chromatography (only wild-type), and gel filtration
recombinant IcmB is purified using Q-Sepharose, and two Mono Q column chromatography
using ammonium sulfate precipitation, DEAE, Q-sepharose, superdex, gel electrophoresis, and B12 affinity column chromatography
-
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration
-
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration
-
Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration
-
