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Literature summary for 5.4.99.13 extracted from
- Cloning, sequencing, expression, and insertional inactivation of the gene for the large subunit of the coenzyme B12-dependent isobutyryl-CoA mutase from Streptomyces cinnamonensis (1998), J. Biol. Chem., 273, 6508-6517.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| IcmA, expression in Escherichia coli and Streptomyces lividans, IcmA shows 70% of homology to the methylmalonyl-CoA mutase large subunit from Streptomyces cinnamonensis | Streptomyces virginiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | disruption of IcmA in Streptomyces cinnamonensis | Streptomyces virginiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
IcmA, 1 * 65000 + several proteins of lower mass, SDS-PAGE | Streptomyces virginiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropanoyl-CoA | Streptomyces virginiae | biosynthesis of the antibiotic monesin-A | butanoyl-CoA | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces virginiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation, DEAE, Q-sepharose, superdex, gel electrophoresis, and B12 affinity column chromatography | Streptomyces virginiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.023 | - |
with butanoyl-CoA as the substrate from extracts of Streptomyces cinnamonensis | Streptomyces virginiae |
| 1 | - |
activity reconstituted by mixing recombinant His6-IcmA produced in Escherichia coli with IcmB isolated from Streptomyces cinnamonensis | Streptomyces virginiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropanoyl-CoA | - |
Streptomyces virginiae | butanoyl-CoA | - |
r | |
| 2-methylpropanoyl-CoA | biosynthesis of the antibiotic monesin-A | Streptomyces virginiae | butanoyl-CoA | - |
r | |
| isobutyrylcarba(dethia)-CoA | stable toward hydrolysis facilitating estimation of the equilibrium constant | Streptomyces virginiae | butyrylcarba(dethia)-CoA | stable toward hydrolysis facilitating estimation of the equilibrium constant | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | IcmA, 1 * 65000 + several proteins of lower mass, SDS-PAGE | Streptomyces virginiae |
| More | IcmB contains coenzyme B12 binding domain | Streptomyces virginiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICM | - |
Streptomyces virginiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces virginiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable at room temperature for several hours | Streptomyces virginiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Streptomyces virginiae |
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Release 2023.1 (January 2023)
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