5.4.3.11: phenylalanine aminomutase (D-beta-phenylalanine forming)

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Word Map on EC 5.4.3.11

5.4.3.11

pantoea

synthesis

agglomerans

unnatural

taxus

stereochemistry

isomerizes

isomerization

drug development

Reaction

Synonyms

admH, EncP, HitA, methylidene imidazolone-dependent phenylalanine aminomutase, MIO-dependent phenylalanine ammonia lyase, PAM, PaPAM, phenylalanine 2,3-aminomutase, phenylalanine aminomutase, phenylalanine-2,3-aminomutase

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.3 Transferring amino groups

5.4.3.11 phenylalanine aminomutase (D-beta-phenylalanine forming)

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Results	in table
2	Activating Compound
24	AA Sequence
4	Application
7	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
9	General Information
1	General Stability
24	kcat/KM [mM/s]
25	KM Value [mM]
1	Metals/Ions
5	Natural Substrates/ Products (Substrates)
12	Organism
4	pH Optimum
1	pH Stability
5	Protein Variants
4	Purification (Commentary)
3	Reaction
1	Reaction Type
11	Reference
53	Substrates and Products (Substrate)
2	Subunits
28	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
4	Temperature Stability [°C]
24	Turnover Number [1/s]

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Cloned on EC 5.4.3.11 - phenylalanine aminomutase (D-beta-phenylalanine forming)

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a 1639 bps long synthetic gene, recombinant overexpression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, real-time PCR enzyme expression analysis, method evaluation, optimization, and upscaling to high level protein expression. 37°C is the best temperature for maximum protein production. Subcloning in Escherichia coli strain XL-1 Blue

Pantoea agglomerans

Q84FL5

749387

expression in Escherichia coli

2 entries

gene hitA, cDNA library construction, genome mining of hitachimycin biosynthetic gene cluster, DNA and amino acid sequence determination and analysis. The identified gene cluster is responsible for the biosynthesis of hitachimycin. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Embleya scabrispora

A0A0F7R1D9

747538

gene pam, functional recombinant expression from plasmid pET28a in Escherichia coli strain BL21

Streptomyces maritimus

747520

gene papam, recombinant expression of C-terminally His6-tagged PaPAM in Escherichia coli strain BL21(DE3)

Pantoea agglomerans

Q84FL5

746575

recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS

Pantoea agglomerans

Q84FL5

749290