5.4.3.11: phenylalanine aminomutase (D-beta-phenylalanine forming)

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For detailed information about phenylalanine aminomutase (D-beta-phenylalanine forming), go to the full flat file.

Word Map on EC 5.4.3.11

5.4.3.11

pantoea

synthesis

agglomerans

unnatural

taxus

stereochemistry

isomerizes

isomerization

drug development

Reaction

Synonyms

admH, EncP, HitA, methylidene imidazolone-dependent phenylalanine aminomutase, MIO-dependent phenylalanine ammonia lyase, PAM, PaPAM, phenylalanine 2,3-aminomutase, phenylalanine aminomutase, phenylalanine-2,3-aminomutase

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.3 Transferring amino groups

5.4.3.11 phenylalanine aminomutase (D-beta-phenylalanine forming)

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Results	in table
2	Activating Compound
24	AA Sequence
4	Application
7	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
9	General Information
1	General Stability
24	kcat/KM [mM/s]
25	KM Value [mM]
1	Metals/Ions
5	Natural Substrates/ Products (Substrates)
12	Organism
4	pH Optimum
1	pH Stability
5	Protein Variants
4	Purification (Commentary)
3	Reaction
1	Reaction Type
11	Reference
53	Substrates and Products (Substrate)
2	Subunits
28	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
4	Temperature Stability [°C]
24	Turnover Number [1/s]

Purification

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Purification on EC 5.4.3.11 - phenylalanine aminomutase (D-beta-phenylalanine forming)

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recombinant C-terminally His6-tagged PaPAM from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and ultrafiltration, to over 95% purity

Pantoea agglomerans

Q84FL5

746575

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration

Embleya scabrispora

A0A0F7R1D9

747538

recombinant N-terminally His10-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dialysis, and ultrafiltration

Pantoea agglomerans

Q84FL5

749290, 749387