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Literature summary for 5.4.3.11 extracted from

  • Chesters, C.; Wilding, M.; Goodall, M.; Micklefield, J.
    Thermal bifunctionality of bacterial phenylalanine aminomutase and ammonia lyase enzymes (2012), Angew. Chem. Int. Ed. Engl., 51, 4344-4348.
    View publication on PubMed
Search for more literature for 5.4.3.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea agglomerans
expression in Escherichia coli Streptomyces maritimus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 L-phenylalanine 25°C, pH not specified in the publication Streptomyces maritimus
0.027
-
 D-beta-phenylalanine 25°C, pH not specified in the publication Pantoea agglomerans
0.032
-
 L-phenylalanine 25°C, pH not specified in the publication Pantoea agglomerans
0.04
-
 D-beta-phenylalanine 25°C, pH not specified in the publication Streptomyces maritimus

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans Q84FL5
-
-
Streptomyces maritimus Q9KHJ9 putative
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-beta-phenylalanine
-
 Pantoea agglomerans L-phenylalanine
-
 r
D-beta-phenylalanine
-
 Streptomyces maritimus L-phenylalanine
-
 r
L-phenylalanine
-
 Pantoea agglomerans D-beta-phenylalanine
-
 r
L-phenylalanine
-
 Streptomyces maritimus D-beta-phenylalanine
-
 r
additional information enzyme additionally displays ammonia lyase activity, EC. 4.3.1.24, catalyzing the elimination of ammonia from L-phenylalanine to give trans-cinnamate. No trans-cinnamate is produced upon incubation with L-phenylalanine at 25°C, whilst very low lyase activity can be observed at 30°C Pantoea agglomerans ?
-
 ?
additional information enzyme additionally displays ammonia lyase activity, EC. 4.3.1.24, catalyzing the elimination of ammonia from L-phenylalanine to give trans-cinnamate. No trans-cinnamate is produced upon incubation with L-phenylalanine at 25°C, whilst very low lyase activity can be observed at 30°C Streptomyces maritimus ?
-
 ?

Synonyms

Synonyms Comment Organism
EncP
-
 Streptomyces maritimus
PAM
-
 Pantoea agglomerans
PAM
-
 Streptomyces maritimus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 the ammonia lyase activity shows a temperature maximum at 70°C Pantoea agglomerans
additional information
-
 the ammonia lyase activity shows a temperature maximum at about 75°C Streptomyces maritimus
45
-
 aminomutase activity Streptomyces maritimus
50
-
 aminomutase activity Pantoea agglomerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 3 h, no loss of activity Streptomyces maritimus
65
-
 3 h, 60% residual activity Streptomyces maritimus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.059
-
 D-beta-phenylalanine 25°C, pH not specified in the publication Pantoea agglomerans
0.087
-
 L-phenylalanine 25°C, pH not specified in the publication Pantoea agglomerans
0.159
-
 L-phenylalanine 25°C, pH not specified in the publication Streptomyces maritimus
0.22
-
 D-beta-phenylalanine 25°C, pH not specified in the publication Streptomyces maritimus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
 D-beta-phenylalanine 25°C, pH not specified in the publication Pantoea agglomerans
2.73
-
 L-phenylalanine 25°C, pH not specified in the publication Pantoea agglomerans
5.53
-
 D-beta-phenylalanine 25°C, pH not specified in the publication Streptomyces maritimus
7.19
-
 L-phenylalanine 25°C, pH not specified in the publication Streptomyces maritimus