5.3.3.1: steroid DELTA-isomerase

This is an abbreviated version!
For detailed information about steroid DELTA-isomerase, go to the full flat file.

Word Map on EC 5.3.3.1

5.3.3.1

steroidogenic

progesterone

adrenal

steroidogenesis

androgen

p450scc

leydig

star

testicular

pregnenolone

17beta-hsd

lh

aromatase

5-3-ketosteroids

gonad

testosteroni

granulosa

dehydroepiandrosterone

luteal

androstenedione

17beta-hydroxysteroid

dheas

theca

p450arom

17alpha-hydroxylase

neurosteroids

cyp11a1

trilostane

19-nortestosterone

dienolate

5alpha-reductase

equilenin

low-barrier

17alpha-hydroxylase/17,20-lyase

interna

3betahsd

delta5-3beta-hydroxysteroid

intracrine

double-bond

hsd3b1

talalay

20alpha-hsd

beta-proton

Reaction

Synonyms

3-Keto-DELTA5-steroid isomerase, 3-Ketosteroid DELTA5-->DELTA4-isomerase, 3-ketosteroid isomerase, 3-Oxo steroid DELTA4-DELTA5-isomerase, 3-Oxo-delta5 steroid isomerase, 3-oxo-DELTA5-steroid isomerase, 3-Oxosteroid DELTA4-DELTA5-isomerase, 3-Oxosteroid DELTA5-DELTA4-isomerase, 3-Oxosteroid isomerase, 3beta-HSD, 3beta-HSD/isomerase, 3beta-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase, 3beta-hydroxysteroid dehydrogenase/isomerase, 3beta-hydroxysteroid dehydrogenase/isomerase type 1, 3beta-hydroxysteroid dehydrogenase/isomerase type 2, 3KSI, 5-Ene-4-ene isomerase, 5-Pregnene-3,20-dione isomerase, delta 5-3-ketosteroid isomerase, DELTA-3-ketosteroid isomerase, Delta-5-3-ketosteroid isomerase, DELTA5(or DELTA4)-3-keto steroid isomerase, DELTA5-3-keto steroid isomerase, DELTA5-3-ketosteroid isomerase, DELTA5-3-oxosteroid isomerase, DELTA5-ketosteroid isomerase, DELTA5-steroid isomerase, glutathione transferase A3-3, GST A3-3, Hydroxysteroid isomerase, Isomerase, steroid DELTA, ketosteroid isomerase, KSI, More, Steroid 5-->4-isomerase, Steroid isomerase, TI, TI-WT, type I 3beta-hydroxysteroid dehydrogenase/isomerase

ECTree

5 Isomerases

5.3 Intramolecular oxidoreductases

5.3.3 Transposing C=C bonds

5.3.3.1 steroid DELTA-isomerase

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Results	in table
1	Activating Compound
410	AA Sequence
1	CAS Registry Number
17	Cloned(Commentary)
7	Cofactor
25	Crystallization (Commentary)
270	Disease/ Diagnostics
1	Expression
1	General Information
5	General Stability
5	IC50 Value
72	Inhibitors
7	kcat/KM [mM/s]
22	Ki Value [mM]
99	KM Value [mM]
10	Localization
25	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
98	Organism
16	Pathway
196	PDB ID
8	pH Optimum
3	pH Range
90	Protein Variants
25	Purification (Commentary)
7	Reaction
4	Reaction Type
97	Reference
8	Renatured (Commentary)
41	Source Tissue
6	Specific Activity [micromol/min/mg]
4	Storage Stability
91	Substrates and Products (Substrate)
15	Subunits
112	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
86	Turnover Number [1/s]

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Cloned on EC 5.3.3.1 - steroid DELTA-isomerase

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2 entries

D38A, D38A/Y14F, D38A/Y55F and D38A/D99N proteins are expressed in Epicurian Coli XL1-Blue cells

Comamonas testosteroni

649966

expressed in Escherichia coli BL21(DE3) cells

Pseudomonas putida

P07445

748656

expressed in HeLa cells

Homo sapiens

2906

expression of C-terminally His6-tagged enzyme as insoluble protein in inclusion bodies in Escherichia coli strain BLR(DE3)

Comamonas testosteroni

P00947

678842, 678846, 678900

expression of mutant enzymes D40N and Y16F in Escherichia col BL21

Pseudomonas putida

651623

expression of type 1 isozyme, wild-type and mutant enzymes, in Spodoptera frugiperda Sf9 cells via baculovirus infection

Homo sapiens

P14060, P26439

670028

expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha

2 entries

insect Sf9 cells transfected with recombinant baculovirus

Homo sapiens

389386

mutant enzymes D36A/K37R, D241N, D257L, D258L and D265N are expressed in a baculovirus/Sf9 cell system

Homo sapiens

652452

overexpression in Escherichia coli

Pseudomonas putida

692914

overexpression in Escherichia coli strain BL21 (DE3)

Pseudomonas putida

679832

Overexpression of recombinant 15N-KSI from Escherichia coli BL21 DE3

Pseudomonas putida

693273