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5.3.3.1: steroid DELTA-isomerase

This is an abbreviated version!
For detailed information about steroid DELTA-isomerase, go to the full flat file.

Word Map on EC 5.3.3.1

Reaction

a 3-oxo-DELTA5-steroid
=
a 3-oxo-DELTA4-steroid

Synonyms

3-Keto-DELTA5-steroid isomerase, 3-Ketosteroid DELTA5-->DELTA4-isomerase, 3-ketosteroid isomerase, 3-Oxo steroid DELTA4-DELTA5-isomerase, 3-Oxo-delta5 steroid isomerase, 3-oxo-DELTA5-steroid isomerase, 3-Oxosteroid DELTA4-DELTA5-isomerase, 3-Oxosteroid DELTA5-DELTA4-isomerase, 3-Oxosteroid isomerase, 3beta-HSD, 3beta-HSD/isomerase, 3beta-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase, 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase, 3beta-hydroxysteroid dehydrogenase/isomerase, 3beta-hydroxysteroid dehydrogenase/isomerase type 1, 3beta-hydroxysteroid dehydrogenase/isomerase type 2, 3KSI, 5-Ene-4-ene isomerase, 5-Pregnene-3,20-dione isomerase, delta 5-3-ketosteroid isomerase, DELTA-3-ketosteroid isomerase, Delta-5-3-ketosteroid isomerase, DELTA5(or DELTA4)-3-keto steroid isomerase, DELTA5-3-keto steroid isomerase, DELTA5-3-ketosteroid isomerase, DELTA5-3-oxosteroid isomerase, DELTA5-ketosteroid isomerase, DELTA5-steroid isomerase, glutathione transferase A3-3, GST A3-3, Hydroxysteroid isomerase, Isomerase, steroid DELTA, ketosteroid isomerase, KSI, More, Steroid 5-->4-isomerase, Steroid isomerase, TI, TI-WT, type I 3beta-hydroxysteroid dehydrogenase/isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.3 Transposing C=C bonds
                5.3.3.1 steroid DELTA-isomerase

Molecular Weight

Molecular Weight on EC 5.3.3.1 - steroid DELTA-isomerase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11000
-
2 * 11000, SDS-PAGE. At concentrations below 1 mg/ml the enzyme exists as a dimer of MW 26800, determined by gel filtration and ultracentrifugation studies. Concentration-dependent association occurs at higher enzyme concentrations
14000
-
2 * 14000, mutant enzymnes R72A, E118A, N120A and E18A/N120A, SDS-PAGE
14520
18700
-
protein concentration 2 ng/ml, gel filtration
19000
-
4 * 19000, enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase, SDS-PAGE
20900
-
protein concentration 20 ng/ml, gel filtration
23100
-
protein concentration 200 ng/ml, gel filtration
24900
-
protein concentration 0.002 mg/ml, gel filtration
26000
-
equilibrium sedimentation at 20 C
26000 - 68000
-
protein concentration 0.043 mg/ml-15.6 mg/ml. At concentrations below 1 mg/ml the enzyme exists as a dimer of MW 26800, determined by gel filtration and ultracentrifugation studies. Concentration-dependent association occurs at higher enzyme concentrations. The latter value represents the weight avarage molecular weight of two or more polymerization species in rapid equilibrium, rather than a discrete polymeric form of the enzyme
26800
-
protein concentration 0.02 mg/ml, gel filtration
27700
-
equilibrium sedimentation at 4 C
300000
-
DELTA5-3-beta-hydroxysteroid dehydrogenase-isomerase system, gel filtration
38800
-
x * 38800, truncated soluble enzyme DEALTA283-310
40000
-
x * 40000, DELTA5-3-beta-hydroxysteroid dehydrogenase-isomerase system, SDS-PAGE
40800
-
low speed sedimentation without reaching equilibrium
41000
-
2 * 41000, SDS-PAGE
41874
-
x * 41874, calculation from nucleotide sequence
42000
-
x * 42000, wild-type enzyme
45000
-
2 * 45000, 3beta-hydroxy-5-ene steroid dehydrogenase/isomerase enzyme system, SDS-PAGE
46800
-
x * 46800, SDS-PAGE, enzyme with steroid DELTA-isomerase activity and 3beta-hydroxy-5-ene-steroid dehydrogenase activity
76000
-
enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase, gel filtration
77000
-
enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase gel filtration
81000
-
3beta-hydroxy-5-ene steroid dehydrogenase/steroid DELTA-isomerase system, gel filtration