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5.1.3.2: UDP-glucose 4-epimerase

This is an abbreviated version!
For detailed information about UDP-glucose 4-epimerase, go to the full flat file.

Word Map on EC 5.1.3.2

Reaction

UDP-alpha-D-glucose
=
UDP-alpha-D-galactose

Synonyms

4-Epimerase, 6xHis-rGalE, ABD1_580, An14g03820, AtUGE1, AtUGE2, AtUGE3, AtUGE4, AtUGE5, CaGAL10, CapD, epimerase Ab-WbjB, Epimerase, uridine diphosphoglucose, fnlA, GAL10, Gal10p, Galactowaldenase, GalE, galE-1, galE-2, galE1, GalESp1, GalESp2, GNE, GNE2, H3634, HvUGE1, HvUGE2, HvUGE3, MdUGE1, More, OsUGE-1, OsUGE1, PsUGE1, Rdh1, rGalE, Rv3634c, TbGalE, TM0509, TMGalE, UDP-D-galactose 4-epimerase, UDP-D-glucose/UDP-D-galactose 4-epimerase, UDP-Gal 4-epimerase, UDP-Gal/Glc 4-epimerase, UDP-galactose 4'-epimerase, UDP-galactose 4-epimerase, UDP-galactose-4'-epimerase, UDP-galactose-4-epimerase, UDP-Glc 4-epimerase, UDP-Glc(NAc) 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-GlcNAc/Glc 4-epimerase, UDP-glucose 4'-epimerase, UDP-glucose 4-epimerase, UDP-glucose 4-epimerase 1, UDP-glucose 4-epimerase 4, UDP-glucose epimerase, UDP-glucose-4-epimerase, UDP-glucose/-galactose 4-epimerase, UDP-hexose 4-epimerase, UDP-N-acetyl-glucosamine 4,6-dehydratase, UDP-sugar 4-epimerase, UDP-Xyl 4-epimerase, UDP-xylose 4-epimerase, UDPG-4-epimerase, UDPgalactose 4-epimerase, UGE, UGE-1, UGE1, UGE1:GUS, Uge1p, UGE2, UGE3, UGE3:GUS, UGE4, UGE5, UgeA, Uridine diphosphate galactose 4-epimerase, Uridine diphosphate glucose 4-epimerase, uridine diphosphate-galactose-4'-epimerase, Uridine diphospho-galactose-4-epimerase, Uridine diphosphogalactose-4-epimerase, Uridine diphosphoglucose 4-epimerase, Uridine diphosphoglucose epimerase, uridine-diphospho-glucose 4-epimerase, WbjB

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.2 UDP-glucose 4-epimerase

Purification

Purification on EC 5.1.3.2 - UDP-glucose 4-epimerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration
Q81JK4, Q81K34
by using nickel-NTA affinity chromatography
-
DEAE-Sepharose anion exchange chromatography
-
DEAE-Toyopearl column chromatography and Superdex 200 gel filtration
fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker
-
MalE-Gne fusion protein overproduced in Escherichia coli
-
Ni+ affinity column chromatography
Ni2+-NTA affinity chromatography
-
nickel agarose column chromatography
-
partial
Trigonella sp.
-
purification of the native enzyme from pea sprouts by ion exchange chromatography and gel filtration, further purification on a hydroxyapatite column, purification of recombinant enzymes by ion exchange chromatography
purification of the recombinant Arabidopsis enzyme by Ni-NTA affinity chromatography
purification of the recombinant enzyme by Ni-NTA affinity chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3)
Q81JK4, Q81K34
recombinant fusion enzyme 2.33fold from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, native UGE1 334fold from sprouts by ammonium sulfate fractionation and a chromatographic procedure
recombinant His-tagged enzyme by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged isozymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21-Gold (DE3)
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-Gold by nickel affinity chromatography and ultrafiltration
recombinant wild-type and mutant enzymes S132A, Y157F, S132A/Y157F and C307Y
-