General Stability | Organism |
---|---|
glycerol is required for stability to freezing and thawing | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
UDPgalactose | mammary enzyme | Bos taurus | |
0.017 | - |
UDPgalactose | liver enzyme | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36700 | - |
2 * 36700, SDS-PAGE | Bos taurus |
37100 | - |
2 * 37100, SDS-PAGE | Bos taurus |
40000 | - |
mammary and liver enzyme, gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
mammary gland | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
66 | - |
liver | Bos taurus |
210 | - |
mammary | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, dilute solutions of highly purified liver or mammary enzyme are very stable in the presence of 20 to 200 mM potassium phosphate, 20% glycerol, 1 mM mercaptoethanol, at pH 7.6, 10% loss of activity after 6 months | Bos taurus |
4°C, dilute solutions of highly purified liver or mammary enzyme are very stable in the presence of 20 to 200 mM potassium phosphate, 20% glycerol, 1 mM mercaptoethanol, at pH 7.6 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose | - |
Bos taurus | UDP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 2 * 36700, SDS-PAGE | Bos taurus |
monomer | 2 * 37100, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
2 h, stable in presence of 1% bovine serum albumin, 0.5 mM dithiothreitol or 1 mM mercaptoethanol | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | Km: 0.29 mM for the thyroid enzyme | Bos taurus | |
NAD+ | absolute requirement for NAD+. Km: 0.27 mM for the liver enzyme, Km: 0.28 mM for the mammary enzyme | Bos taurus |