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Literature summary for 5.1.3.2 extracted from

  • Geren, C.R.; Ebner, K.E.
    Purification and characterization of UDP-galactose-4-epimerase from bovine tissues (1977), J. Biol. Chem., 252, 2082-2088.
    View publication on PubMed

General Stability

General Stability Organism
glycerol is required for stability to freezing and thawing Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
UDPgalactose mammary enzyme Bos taurus
0.017
-
UDPgalactose liver enzyme Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36700
-
2 * 36700, SDS-PAGE Bos taurus
37100
-
2 * 37100, SDS-PAGE Bos taurus
40000
-
mammary and liver enzyme, gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-
mammary gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66
-
liver Bos taurus
210
-
mammary Bos taurus

Storage Stability

Storage Stability Organism
-20°C, dilute solutions of highly purified liver or mammary enzyme are very stable in the presence of 20 to 200 mM potassium phosphate, 20% glycerol, 1 mM mercaptoethanol, at pH 7.6, 10% loss of activity after 6 months Bos taurus
4°C, dilute solutions of highly purified liver or mammary enzyme are very stable in the presence of 20 to 200 mM potassium phosphate, 20% glycerol, 1 mM mercaptoethanol, at pH 7.6 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose
-
Bos taurus UDP-glucose
-
?

Subunits

Subunits Comment Organism
monomer 2 * 36700, SDS-PAGE Bos taurus
monomer 2 * 37100, SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
24
-
2 h, stable in presence of 1% bovine serum albumin, 0.5 mM dithiothreitol or 1 mM mercaptoethanol Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+ Km: 0.29 mM for the thyroid enzyme Bos taurus
NAD+ absolute requirement for NAD+. Km: 0.27 mM for the liver enzyme, Km: 0.28 mM for the mammary enzyme Bos taurus