Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
UDPgalactose | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
2 * 78000, SDS-PAGE | Saccharomyces cerevisiae |
183000 | 187000 | sucrose density gradient centrifugation, gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.8 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose | - |
Saccharomyces cerevisiae | UDP-glucose | - |
? | |
UDP-galactose | - |
Saccharomyces cerevisiae 106-3D | UDP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 78000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
53% loss of activity within 20 min, partial protection by NAD+, up to a concentration of 7 mM | Saccharomyces cerevisiae |
37 | - |
85% loss of activity within 20 min, partial protection by NAD+, up to a concentration of 7 mM | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.8 | - |
UDPgalactose | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.1 | 6.7: 91% of maximal activity, 8.1: 88% of maximal activity | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | each dimer contains one molecule of NAD+ tightly bound | Saccharomyces cerevisiae |