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5.1.3.2: UDP-glucose 4-epimerase

This is an abbreviated version!
For detailed information about UDP-glucose 4-epimerase, go to the full flat file.

Word Map on EC 5.1.3.2

Reaction

UDP-alpha-D-glucose
=
UDP-alpha-D-galactose

Synonyms

4-Epimerase, 6xHis-rGalE, ABD1_580, An14g03820, AtUGE1, AtUGE2, AtUGE3, AtUGE4, AtUGE5, CaGAL10, CapD, epimerase Ab-WbjB, Epimerase, uridine diphosphoglucose, fnlA, GAL10, Gal10p, Galactowaldenase, GalE, galE-1, galE-2, galE1, GalESp1, GalESp2, GNE, GNE2, H3634, HvUGE1, HvUGE2, HvUGE3, MdUGE1, More, OsUGE-1, OsUGE1, PsUGE1, Rdh1, rGalE, Rv3634c, TbGalE, TM0509, TMGalE, UDP-D-galactose 4-epimerase, UDP-D-glucose/UDP-D-galactose 4-epimerase, UDP-Gal 4-epimerase, UDP-Gal/Glc 4-epimerase, UDP-galactose 4'-epimerase, UDP-galactose 4-epimerase, UDP-galactose-4'-epimerase, UDP-galactose-4-epimerase, UDP-Glc 4-epimerase, UDP-Glc(NAc) 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-GlcNAc/Glc 4-epimerase, UDP-glucose 4'-epimerase, UDP-glucose 4-epimerase, UDP-glucose 4-epimerase 1, UDP-glucose 4-epimerase 4, UDP-glucose epimerase, UDP-glucose-4-epimerase, UDP-glucose/-galactose 4-epimerase, UDP-hexose 4-epimerase, UDP-N-acetyl-glucosamine 4,6-dehydratase, UDP-sugar 4-epimerase, UDP-Xyl 4-epimerase, UDP-xylose 4-epimerase, UDPG-4-epimerase, UDPgalactose 4-epimerase, UGE, UGE-1, UGE1, UGE1:GUS, Uge1p, UGE2, UGE3, UGE3:GUS, UGE4, UGE5, UgeA, Uridine diphosphate galactose 4-epimerase, Uridine diphosphate glucose 4-epimerase, uridine diphosphate-galactose-4'-epimerase, Uridine diphospho-galactose-4-epimerase, Uridine diphosphogalactose-4-epimerase, Uridine diphosphoglucose 4-epimerase, Uridine diphosphoglucose epimerase, uridine-diphospho-glucose 4-epimerase, WbjB

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.2 UDP-glucose 4-epimerase

Cloned

Cloned on EC 5.1.3.2 - UDP-glucose 4-epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, quantitative real-time reverse transcription PCR enzyme expression analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli ER2566 cells
expressed in Escherichia coli Rosetta cells and in a gal10-null strain of Saccharomyces cerevisiae strain JFy3835
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expression in Agrobacterium tumefaciens
expression in Escherichia coli
expression in Escherichia coli and Saccharomyces cerevisiae
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expression in GALE-null line of Chineses hamster ovary cells. GALE-null cells accumulats abnormally high levels of Hal-1-P and UDP-Gal and abnormally low levels of UDP-Glc and UDP-GLcNAc in the presence of galactose. Human GALE expression corrects each of theses defects
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expression in GALE-null line of Chinesese hamster ovary cells. Enzyme from Escherichia coli can not restore the metabolic balance, because unlike the mammalian enzyme it can not catalyze the interconversion of UDP-GalNAc and UDP-GlcNAc
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expression in in Escherichia coli
expression in Pichia pastoris
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Expression in Saccharomyces cerevisiae EBY100.
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expression in Sinorhizobium meliloti
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expression of GFP-tagged wild-type enzyme in HEK-293T cells, and transient expression of myc-tagged GALER239W and GALEG302D, and of myc- or FLAG-tagged GALE165K and GALEW336X in HEK-293T cells and in GALE-null ldlD cells
expression of His-tagged enzyme
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expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Gold
expression of the enzyme obtained from HB8 strain in Escherichia coli as His-tagged protein
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fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker
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galE transcription exhibits a distinct expressionprofile under different culture conditions, mapping of galE transcription initiation
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gene BAS5114, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Q81JK4, Q81K34
gene fnlA or ABD1_580, DNA and amino acid sequence determination and analysis, sequence comparisons, the gene belongs to the components of polysaccharide biosynthesis cluster RGP01, overview, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS
gene galE, functional expression of N-termminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene GALE, real-time quantitative PCR expression analysis in chondrocytes
gene galE, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and functional complementation of enzyme-deficient Escherichia coli strain BW25113 DELTAgalE
gene galE-1, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene galE1 or Rv3634c, sequence comparisons, e.g. with the enzyme from Mycobacterium smegmatis strain mc2 4517, recombinant expression of His-tagged enzyme in Escherichia coli strains BL21(DE3)pLysS, BL21(DE3), BL21-CodonPlus(DE3)-RIPL, OverExpress C41(DE3), or OverExpress C43(DE3)pLysS, and recombinant expression of the enzyme in Mycobacterium smegmatis strain mc2 4517, none of these approaches leads to expression of the enzyme in the soluble form, except for strain BL21(DE3)pLysS, recombinant expression of enzyme mutants
gene galEsp2 or galE-2, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene OsUGE-1, phylogenetic analysis of the UGEs from Oryza sativa, recombinant overexpression in Oryza sativa via Agrobacterium tumefaciens-mediated transformation, real-time PCR enzyme expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene UGE1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the enzyme fused to thioredoxin and His6 in Escherichia coli strain BL21(DE3)
gene uge1, is constitutively expressed, recombinant expression in Escherichia coli strain XL-1 blue
gene UGE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain
gene UGE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain Transetta (DE3)
gene ugeA, DNA and amino acid sequence determination and analysis, cytoplasmic expression of GFP-tagged UgeA in Aspergillus nidulans from a plasmid under control of the wild-type promoter, expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli
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genes uge3 and uge5, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), expression as fluorescence labeled proteins uge3-gfp and uge5-rfp in Aspergillus. fumigatus strain Af293
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mutant enzymes S132A, Y157F, S132A/Y157F and C307Y are expressed in a null-background strain of Saccharomyces cerevisiae. S132a/Y157F and C307Y are also overexpressed in Pichia pastoris
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overexpression in Escherichia coli
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overproduction of MalE-Gne fusion protein in Escherichia coli
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recombinant enzyme overexpression in Thermus thermophilus strain HB27 to an increased capacity of biofilm production, expression of His-tagged enzyme
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recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21-Gold (DE3)
recombinant protein is expressed in Escherichia coli
recombinant protein is expressed in Escherichia coli with C-terminal His-tag
To explore the quantitative relationship between GALE activity and galactose metabolism and sensitivity in yeast, a strain is generated in which the expression of GAL10, encoding GALE, was regulated by doxycycline. In brief, a doxycycline-repressible promoter is introduced just upstream of the GAL10 coding sequence in a strain (JFy4763) that was otherwise wild type for all Leloir pathway enzymes and that was engineered to express the appropriate doxycycline-responsive transcriptional activator and repressor protein moieties.
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