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Literature summary for 5.1.3.2 extracted from
- Novel activity of UDP-galactose-4-epimerase for free monosaccharide and activity improvement by active site-saturation mutagenesis (2011), Appl. Biochem. Biotechnol., 163, 444-451.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N179S | the 4-epimerization of tagatose is enhanced 2fold in this mutant | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.255 | - |
UDP-galactose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli |  |
| 2 | 37 | D-tagatose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
| 26.4 | - |
D-galactose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P09147 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni+ affinity column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | - |
Escherichia coli | D-tagatose | - |
? | |
| D-galactose | the catalytic efficiencies of GalE for D-galactose is much lower than for UDP-galactose | Escherichia coli | D-glucose | - |
? | |
| D-glucose | - |
Escherichia coli | D-galactose | - |
? | |
| D-psicose | - |
Escherichia coli | D-sorbose | - |
? | |
| D-sorbose | - |
Escherichia coli | D-psicose | - |
? | |
| D-tagatose | the catalytic efficiencies of GalE for D-tagatose is much lower than for UDP-galactose | Escherichia coli | D-fructose | - |
? | |
| additional information | no 4-epimerization activity is found for allose, gulose, altrose, idose, mannose, and talose | Escherichia coli | ? | - |
? | |
| UDP-galactose | - |
Escherichia coli | UDP-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GalE | - |
Escherichia coli |
| UDP-galactose-4-epimerase | - |
Escherichia coli |
| Uridine diphosphogalactose-4-epimerase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00026 | - |
D-galactose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
| 0.00545 | - |
D-tagatose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
| 750 | - |
UDP-galactose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000097 | - |
D-galactose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
| 0.000023 | - |
D-tagatose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
| 3333 | - |
UDP-galactose | in McIlvaine buffer (pH 7.5), at 35°C | Escherichia coli | |
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