5.1.2.1: lactate racemase
This is an abbreviated version!
For detailed information about lactate racemase, go to the full flat file.
Word Map on EC 5.1.2.1
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5.1.2.1
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lactobacillus
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plantarum
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racemization
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pincer
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hydride
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organometallic
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nickel-containing
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proton-coupled
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nife-hydrogenase
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sacrificial
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insertase
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ni-dependent
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sakei
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pyridinium
- 5.1.2.1
- lactobacillus
- plantarum
-
racemization
-
pincer
-
hydride
-
organometallic
-
nickel-containing
-
proton-coupled
- nife-hydrogenase
-
sacrificial
-
insertase
-
ni-dependent
- sakei
-
pyridinium
Reaction
Synonyms
Hydroxyacid racemase, lactate racemase, Lactic acid racemase, Lacticoracemase, LAR, LARa, nickel-dependent lactate racemase, Racemase, lactate
ECTree
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Engineering
Engineering on EC 5.1.2.1 - lactate racemase
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additional information
construction of an engineered Lactobacillus plantarum strain NCIMB 8826, which enables the production of optically pure L-lactate from raw starch for costeffective lactate production. Disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between D- and L-lactate, completely abolishes D-lactate production. The DELTAldhD DELTAlarA-E mutant produces 87.0 g/l of L-lactate with an optical purity of 99.4%. A plasmid is introduced into the DELTAldhD DELTAlarA-E mutant for the secretion of alpha-amylase from Streptococcus bovis strain 148. The resulting strain produces 50.3 g/l of L-lactate from raw corn starch with a yield of 0.91 g per g of consumed sugar and an optical purity of 98.6%
additional information
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construction of an engineered Lactobacillus plantarum strain NCIMB 8826, which enables the production of optically pure L-lactate from raw starch for costeffective lactate production. Disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between D- and L-lactate, completely abolishes D-lactate production. The DELTAldhD DELTAlarA-E mutant produces 87.0 g/l of L-lactate with an optical purity of 99.4%. A plasmid is introduced into the DELTAldhD DELTAlarA-E mutant for the secretion of alpha-amylase from Streptococcus bovis strain 148. The resulting strain produces 50.3 g/l of L-lactate from raw corn starch with a yield of 0.91 g per g of consumed sugar and an optical purity of 98.6%
additional information
Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1
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construction of an engineered Lactobacillus plantarum strain NCIMB 8826, which enables the production of optically pure L-lactate from raw starch for costeffective lactate production. Disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between D- and L-lactate, completely abolishes D-lactate production. The DELTAldhD DELTAlarA-E mutant produces 87.0 g/l of L-lactate with an optical purity of 99.4%. A plasmid is introduced into the DELTAldhD DELTAlarA-E mutant for the secretion of alpha-amylase from Streptococcus bovis strain 148. The resulting strain produces 50.3 g/l of L-lactate from raw corn starch with a yield of 0.91 g per g of consumed sugar and an optical purity of 98.6%
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