Information on EC 5.1.2.1 - lactate racemase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
5.1.2.1
-
RECOMMENDED NAME
GeneOntology No.
lactate racemase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-Lactate = (R)-lactate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
racemization
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pyruvate metabolism
-
-
lactate fermentation
-
-
SYSTEMATIC NAME
IUBMB Comments
lactate racemase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9024-05-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
ssp. pseudoplantarum
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Halobacterium saccharovorum
-
-
-
Manually annotated by BRENDA team
no activity in Halobacterium salinarum
-
-
-
Manually annotated by BRENDA team
no activity in Haloferax mediterranei
-
-
-
Manually annotated by BRENDA team
strain HD4
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Lactate
L-Lactate
show the reaction diagram
L-lactate
D-lactate
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lactate
D-lactate
show the reaction diagram
-
-
-
-
r
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactor required
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
one molecule of enzyme contains 2 atoms of iron
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-hydroxyquinoline
-
-
Nitroso-R-salts
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
yeast extract (0.1% (w/v)), MgSO4 (2 mM), MnSO4 (2 mM) and ascorbic acid (2 mM) enhance lactate racemase activity in vitro
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17 - 50
D-lactate
70 - 80
L-lactate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.7
D-lactate
Lactobacillus sakei
-
-
6.67
L-lactate
Lactobacillus sakei
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 6.2
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8.5
-
active in the range
7 - 10
-
7: hardly any activity below, 9-10: maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 50
-
15°C: about 40% of maximal activity, 50°C: about 60% of maximal activity
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIB 9385 / NCA 3814)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
gel filtration
50000
-
cellulose chromatography
52000 - 60000
82400
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6
-
highest stability
2173
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
crude extract, 5 min, 96% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli strain TG1
-