5.1.2.1: lactate racemase

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For detailed information about lactate racemase, go to the full flat file.

Word Map on EC 5.1.2.1

5.1.2.1

lactobacillus

plantarum

racemization

pincer

hydride

organometallic

nickel-containing

proton-coupled

nife-hydrogenase

sacrificial

insertase

ni-dependent

sakei

pyridinium

Reaction

Synonyms

Hydroxyacid racemase, lactate racemase, Lactic acid racemase, Lacticoracemase, LAR, LARa, nickel-dependent lactate racemase, Racemase, lactate

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.2 Acting on hydroxy acids and derivatives

5.1.2.1 lactate racemase

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Results	in table
5	Activating Compound
65	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
2	Cofactor
1	Crystallization (Commentary)
2	Expression
20	General Information
1	General Stability
8	Inhibitors
6	KM Value [mM]
6	Metals/Ions
6	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
59	Organism
4	Pathway
5	PDB ID
4	pH Optimum
2	pH Range
1	pH Stability
2	Protein Variants
3	Purification (Commentary)
7	Reaction
1	Reaction Type
29	Reference
2	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
2	Subunits
15	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.1.2.1 - lactate racemase

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purified enzyme, free or Hg-labeled, hanging drop vapour diffusion method, mixing of 0.002 ml of 16 mg/ml protein solution with 0.002 ml of reservoir solution containing 22% PEG monomethyl ether 5000, 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 0.2 M sodium malonate pH 7.0 and 0.02% w/v sodium azide, at 18°C, a few days, X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution

Lactiplantibacillus plantarum

F9USS9

745850