5.1.2.1: lactate racemase

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For detailed information about lactate racemase, go to the full flat file.

Word Map on EC 5.1.2.1

5.1.2.1

lactobacillus

plantarum

racemization

pincer

hydride

organometallic

nickel-containing

proton-coupled

nife-hydrogenase

sacrificial

insertase

ni-dependent

sakei

pyridinium

Reaction

Synonyms

Hydroxyacid racemase, lactate racemase, Lactic acid racemase, Lacticoracemase, LAR, LARa, nickel-dependent lactate racemase, Racemase, lactate

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.2 Acting on hydroxy acids and derivatives

5.1.2.1 lactate racemase

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Results	in table
5	Activating Compound
65	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
2	Cofactor
1	Crystallization (Commentary)
2	Expression
20	General Information
1	General Stability
8	Inhibitors
6	KM Value [mM]
6	Metals/Ions
6	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
59	Organism
4	Pathway
5	PDB ID
4	pH Optimum
2	pH Range
1	pH Stability
2	Protein Variants
3	Purification (Commentary)
7	Reaction
1	Reaction Type
29	Reference
2	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
2	Subunits
15	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Purification

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Purification on EC 5.1.2.1 - lactate racemase

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2 entries

recombinant StrepII-tagged LarA from Lactobacillus lactis by affinity chromatography, gel filtration, and ultrafiltration, to homogeneity. Purification of LarA and LarE can readily be achieved from the strain expressing the entire operon, whereas tagged LarB and LarC can only be purified when the corresponding genes are individually subcloned

Lactiplantibacillus plantarum

F9USS9

745850