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4.6.1.16: tRNA-intron lyase

This is an abbreviated version!
For detailed information about tRNA-intron lyase, go to the full flat file.

Word Map on EC 4.6.1.16

Reaction

pretRNA
=
a 3'-half-tRNA molecule with a 5'-OH end
 +
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
 +
an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus

Synonyms

AF endonuclease, AFN, AF_0900, archaeal pre-tRNA splicing endonuclease, ARMAN-2 EndA, EC 3.1.27.9, EndA, EndATa, epsilon2 endonuclease, Gamma-toxin, hClp1, HEAB, homing endonuclease, HSPC117, I-TevI, intron-encoded endonuclease, MJ1424, More, nuclease, transfer ribonucleate intron endoribo-, pre-tRNA splicing endonuclease, RNA splicing endonuclease, RNA-splicing endonuclease, Sen2, splicing endonuclease, ssol_1416, STK_03580, three-unit tRNA splicing endonuclease, transfer ribonucleate intron endoribonuclease, transfer splicing endonuclease, tRNA anticodon nucleases (ACNs), tRNA endonuclease, tRNA intron endonuclease, tRNA intron-splicing endonuclease, tRNA SEN, tRNA splicing endonuclease, tRNA splicing ligase, tRNA-intron endonuclease, tRNA-splicing endonuclease, tRNATRPintron endonuclease

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.16 tRNA-intron lyase

Cloned

Cloned on EC 4.6.1.16 - tRNA-intron lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
epsilon2 endonuclease, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged wild-type and mutant enzyme
expressed in Escherichia coli Rosetta-gami (DE3) cells
expression in Eschericha coli
-
expression in Escherichia coli
Q975R3; F9VMU0
expression of genes NEQ205 and NEQ261 in Escherichia coli strain BL21(DE3), phylogenetic analysis
-
expression of His6-tagged wild-type and mutant EndAs in Escherichi coli strain Rosetta 2(DE3)
gene AFN, expression of truncated fully active dimeric enzyme form lacking the N-terminal domain in Escherichia coli strain BLR(DE3)
-
gene ARCFU, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene METJA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene SULSO, DNA and amino acid sequence determination and analysis, coexpression of His-tagged SULSO alpha-subunit and untagged beta-subunit in Escherichia coli strain BL21(DE3)
-
gene XENLA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
genes endo1 and endo2, expression as His-tagged subunits forming the heterocomplex
-
genes of all four subunits, each of them being essential
-
overexpression in Escherichia coli
overexpression of EndA in Escherichia coli strain Rosetta 2(DE3)
-
overexpression of wild-type and mutant EndAs in Escherichia coli strain Rosetta 2(DE3)
two distinct genes endA, ORFs ST0358 and STS047, DNA and amino acid sequence determination and analysis of two ORFs encoding the enzyme, one ORF lacks the putative catalytic amino acid residue, individual expression in Escherichia coli reveals that both are components of an enzyme complex
-
two genes coding for different subunits