Cloned (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
H257A | residue of the catalytic triad, 28-fold reduction in activity | Archaeoglobus fulgidus |
H257C | residue of the catalytic triad | Archaeoglobus fulgidus |
H257D | residue of the catalytic triad | Archaeoglobus fulgidus |
H257N | residue of the catalytic triad | Archaeoglobus fulgidus |
K287E | residue of the catalytic triad, no activity in absence of divalent metal ions, activity observed in the presence of specific divalent metal ions | Archaeoglobus fulgidus |
K287Q | residue of the catalytic triad | Archaeoglobus fulgidus |
K287R | residue of the catalytic triad | Archaeoglobus fulgidus |
Y246F | residue of the catalytic triad, 7-fold reduction in activity | Archaeoglobus fulgidus |
Y246F/H257A | residues of the catalytic triad, no activity | Archaeoglobus fulgidus |
Y246H | residue of the catalytic triad | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
pretRNA | wild-type protein, substrate: 5'-AAGCGACCGACC*AUA*GCUGCA-3' or 5'-AAUGCAGCGGUC*AAA*GGUCGC-3' | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AAGCGACCGACC*AUA*GCUGCA-3' + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
5'-AAUGCAGCGGUC*AAA*GGUCGC-3' + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
pretRNA | - |
Archaeoglobus fulgidus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA splicing endonuclease | - |
Archaeoglobus fulgidus |