Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.16 extracted from

  • Zhang, Y.; Li, H.
    Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2(1)2(1)2 (2004), Acta Crystallogr. Sect. D, 60, 447-452.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene AFN, expression of truncated fully active dimeric enzyme form lacking the N-terminal domain in Escherichia coli strain BLR(DE3) Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
truncated active dimeric enzyme form lacking the N-terminal domain, hanging drop vapour diffusion method, 60 mg/ml protein is mixed with reservoir solution conatining 0.1 M sodium cacodylate, pH 6.5-6.8, 20 mM ammonium sulfate, and 0.3-0.4 M sodium acetate, approximately 30 days, X-ray diffraction structure determination and analysis at 2.0 A resolution Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
additional information construction of a truncated fully active dimeric enzyme form lacking the N-terminal domain Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
gene AFN
-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated fully active dimeric enzyme form lacking the N-terminal domain from Escherichia coli strain BLR(DE3) to homogeneity Archaeoglobus fulgidus

Reaction

Reaction Comment Organism Reaction ID
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus active site structure, the catalytic triad is formed by His257, Lys287, and Tyr246 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-pair helix, known as the bulge-helix-bulge motif, BHB motif Archaeoglobus fulgidus ?
-
?

Synonyms

Synonyms Comment Organism
AF endonuclease
-
Archaeoglobus fulgidus
AFN
-
Archaeoglobus fulgidus
RNA-splicing endonuclease
-
Archaeoglobus fulgidus
splicing endonuclease
-
Archaeoglobus fulgidus