Cloned (Comment) | Organism |
---|---|
gene AFN, expression of truncated fully active dimeric enzyme form lacking the N-terminal domain in Escherichia coli strain BLR(DE3) | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
truncated active dimeric enzyme form lacking the N-terminal domain, hanging drop vapour diffusion method, 60 mg/ml protein is mixed with reservoir solution conatining 0.1 M sodium cacodylate, pH 6.5-6.8, 20 mM ammonium sulfate, and 0.3-0.4 M sodium acetate, approximately 30 days, X-ray diffraction structure determination and analysis at 2.0 A resolution | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated fully active dimeric enzyme form lacking the N-terminal domain | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
gene AFN | - |
Purification (Comment) | Organism |
---|---|
recombinant truncated fully active dimeric enzyme form lacking the N-terminal domain from Escherichia coli strain BLR(DE3) to homogeneity | Archaeoglobus fulgidus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus | active site structure, the catalytic triad is formed by His257, Lys287, and Tyr246 | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-pair helix, known as the bulge-helix-bulge motif, BHB motif | Archaeoglobus fulgidus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AF endonuclease | - |
Archaeoglobus fulgidus |
AFN | - |
Archaeoglobus fulgidus |
RNA-splicing endonuclease | - |
Archaeoglobus fulgidus |
splicing endonuclease | - |
Archaeoglobus fulgidus |